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Downregulation of thioredoxin reductase 1 expression in the substantia nigra pars compacta of Parkinson's disease mice

查看全文 作　　者：Zihua [1]Liu;Yuhong [2,3]Jing;Jie [2]Yin;Jiying [2]Mu;Tingting [2]Yao;Liping [1,3]Gao 高影响力作者 机构地区：[1]Institute of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Lanzhou University;[2]Institute of Anatomy and Embryology, School of Basic Medical Sciences, Lanzhou University;[3]Key Laboratory of Preclinical Study for New Drugs of Gansu Province高影响力机构 出　　处：《Neural Regeneration Research》索引2013年第8卷第35期,共9页高影响力期刊 基　　金：supported by the Fundamental Research Funds for the Central Universities, No. lzujbky-2011-83;Project of the International Cooperation and Communion Department of Chinese Education Ministry (46th batch);the Science Foundation of Key Laboratory of Preclinical Study for New Drugs of Gansu Province, No. GSKFKT-0804 摘　　要：Because neurons are susceptible to oxidative damage and thioredoxin reductase 1 is extensively distributed in the central nervous system and has antioxidant properties,we speculated that the enzyme may be involved in the pathogenesis of Parkinson’s disease.A Parkinson’s disease model was produced by intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine into C57BL/6 mice.Real-time reverse transcription-PCR,western blot analysis and colorimetric assay showed that the levels of thioredoxin reductase 1 mRNA and protein were decreased,along with a significant reduction in thioredoxin reductase activity,in the midbrain of Parkinson’s disease mice compared with normal mice.Immunohistochemical staining revealed that the number of thioredoxin reductase 1-positive neurons in the substantia nigra pars compacta of Parkinson’s disease mice was significantly decreased compared with normal mice.These experimental findings suggest that the expression of thioredoxin reductase 1 in the substantia nigra pars compacta of Parkinson’s disease mice is significantly decreased,and that the enzyme may be associated with disease onset. 关 键 词：硫氧还蛋白还原酶 帕金森氏病 黑质致密部 小鼠 逆转录-PCR 蛋白质印迹 阳性神经元 中枢神经系统
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