Antioxidant properties of magnesium lithospermate B contribute to the cardioprotection against myocardial ischemia/reperfusion injury in vivo and in vitro

查看全文 作　　者：Wei [1]Quan;Ying [1]Yin;Miaomiao [1]Xi;Dan [1]Zhou;Yanrong [1]Zhu;Yue [1]Guan;Chao [1]Guo;Yanhua [1]Wang;Jialin [1]Duan;Aidong [1]Wen

机构地区：[1]Department of Pharmacy, Xijing Hospital,Fourth Military Medical University

出　　处：《Journal of Traditional Chinese Medicine》索引2013年第33卷第1期,共7页

基　　金：Supported by the National Natural Science Foundation of China (No. 81173514,No.81001673);the Xijing Research Boosting Program (No. XJZT10D02);the Excellent Civil Service Training Fund of Fourth Military Medical University(No. 4138C4IDK6) 摘　　要：OBJECTIVE: To determine the cardioprotective effect of magnesium lithospermate B (MLB) on myocardial ischemia/reperfusion (MI/R) injury and to investigate the antioxidant potential in vivo and in vitro. METHODS: MI/R injury was induced by the occlusion of left anterior descending coronary artery for 30 min followed by reperfusion for 3 h in rats. After reperfusion, hearts were harvested to assess infarct size, histopathological damages, the levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH) and malondialdehyde (MDA). Blood samples were col- lected to determine serum levels of creatine kinase-MB (CK-MB), cardiac troponin (cTnI) and lactate dehydrogenase (LDH). Furthermore, simulatedischemia/reperfusion (SI/R) injury in vitro was established by oxygen and glucose deprivation (OGD) for 2 h followed by 24-hour recovery period in cardiomyocytes. The activity of LDH in the cultured supernatant and the levels of intracellular reactive oxygen species (ROS), SOD and MDA in cardiomyocytes were also measured. Finally, cardiomyocytes apoptosis was determined with flow cytometry. RESULTS: MLB significantly limited infarct size, ameliorated histopathological damages and prevented leakage of CK-MB, cTnI and LDH. Additionally, SOD, CAT, GPx and GSH activities were notably increased by MLB, along with the MDA content decreased as compared with the model group in rats. In vitro study, MLB also decreased LDH activity in the cultured supernatant, increased SOD activity in cardiomyocytes, reduced intracellular ROS and MDA levels, and significantly suppressed cardiomyocytes apoptosis. CONCLUSION: MLB possessed remarkably cardioprotective effects on MI/R injury in vivo and in vitro. The protection of MLB may contribute to its antioxidant properties. 关键词：缺血再灌注损伤心肌细胞凋亡抗氧化性能丹酚酸B 体外体内谷胱甘肽过氧化物酶镁盐

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Antioxidant properties of magnesium lithospermate B contribute to the cardioprotection against myocardial ischemia/reperfusion injury in vivo and in vitro

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