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Overexpression of lysine specific demethylase 1 predicts worse prognosis in primary hepatocellular carcinoma patients

查看全文 作　　者：Ze-Kun [1]Zhao;Hai-Feng [2]Yu;Dao-Rong [3]Wang;Ping [1]Dong;Lei [1]Chen;Wen-Guang [1]Wu;WenJun [1]Ding;Ying-Bin [1]Liu 高影响力作者 机构地区：[1]Department of General Surgery,Xinhua Hospital Affiliated to Shanghai Jiaotong University;[2]Department of General Surgery,Yixing People's Hospital;[3]Department of General Surgery,Northern Jiangsu People's Hospital高影响力机构 出　　处：《World Journal of Gastroenterology》索引2012年第18卷第45期,共6页高影响力期刊 摘　　要：AIM:To investigate the clinicopathological features and prognostic value of lysine specific demethylase 1(LSD1) in hepatocellular carcinoma(HCC).METHODS:We examined LSD1 expression in 60 paired liver cancer tissues and adjacent noncancerous tissues by quantitative real time polymerase chain reaction(qRT-PCR) and Western blotting.In addition,we analyzed LSD1 expression in 198 HCC samples by immunohistochemistry.The relationship between LSD1 expression,clinicopathological features and patient survival was investigated.RESULTS:Immunohistochemistry,Western blotting,and qRT-PCR consistently confirmed LSD1 overexpression in HCC tissues compared to adjacent non-neoplastic tissues(P < 0.01).Additionally,immunostaining showed more LSD1-positive cells in the higher tumor stage(T3-4) and tumor grade(G3) than in the lower tumor stage(T1-2,P < 0.001) and tumor grade(G1-2,P < 0.001),respectively.Moreover,HCC patients with high LSD1 expression had significantly lower 5-year overall survival rates(P < 0.001) and lower 5-year disease-free survival rates(P < 0.001),respectively.A Cox proportional hazards model further demonstrated that LSD1 over-expression was an independent predictor of poor prognosis for both 5-year disease-free survival [hazards ratio(HR) = 1.426,95%CI:0.672-2.146,P < 0.001] and 5-year overall survival(HR = 2.456,95%CI:1.234-3.932,P < 0.001) in HCC.CONCLUSION:Our data suggest for the first time that the overexpression of LSD1 protein in HCC tissues indicates tumor progression and predicts poor prognosis. 关 键 词：原发性肝癌 过度表达 预测因子 脱甲基酶 赖氨酸 实时定量RT-PCR WESTERN印迹法 预后
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