Study on Application Technology for Tissue Culture and Propagation of Tagetes patula L.

查看全文 作　　者：Rundong [1]FENG;Chunhui [2]LI;Xiaojie [3]TANG

机构地区：[1]Jilin Forestry Technology Extension Station;[2]Fengman District Forestry Bureau of Jilin City;[3]College of Forestry, Beihua University

出　　处：《Agricultural Biotechnology》索引2012年第1卷第2期,共3页

摘　　要：[Objective] This study aimed to investigate the tissue culture and propagation technology in Tagetes patula L. [Method] By using tissue culture technology, different mass fractions of 6-BA and NAA were added to MS medium to compare the effect of different culture medium on the rapid propagation of T. patula L. [Result] Shoot tips or stem segments of T. patula L. were used as explants for tissue culture with an appropriate sterilization time of 8 min; differentiation effect of shoot tips was better than that of stem segments; callus generation rate was high with the high content of growth regulators; MS medium containing 0.1 mg/L NAA and 1.5 mg/L 6-BA was used for subculture proliferation with a subculture period of 4 weeks; rooting rate of plantlet was the maximum (97%) in 1/2MS medium containing 0.2 mg/L NAA, and the root system was relatively developed. [Conclusion] This study provided technical support for the industrialized seedling breeding of T. patula L. 关键词：组织培养技术快繁技术孔雀草快速繁殖 MS培养基应用 6-BA 茎尖分化

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Study on Application Technology for Tissue Culture and Propagation of Tagetes patula L.

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