Paris chinensis dioscin induces G2/M cell cycle arrest and apoptosis in human gastric cancer SGC-7901 cells

查看全文 作　　者：Lin-Lin [1]Gao;Fu-Rong [2]Li;Peng [3]Jiao;Ming-Feng [3]Yang;Xiao-Jun [4]Zhou;Yan-Hong [1]Si;Wen-Jian [4]Jiang;Ting-Ting [4]Zheng

机构地区：[1]Department of Pathology and Pathophysiology, Taishan Medical University, Taian 271000, Shandong Province, China;[2]School of Pharmaceutical Science, Taishan Medical University, Taian 271000, Shandong Province, China;[3]Institute of Basic Medical Sciences, Taishan Medical University, Taian 271000, Shandong Province, China;[4]Department of Clinical Medicine, Taishan Medical University, Taian 271000, Shandong Province, China

出　　处：《World Journal of Gastroenterology》索引2011年第17卷第39期,共7页

基　　金：Supported by The grant from the Department of Education of Shandong Province,China,No.J10LF18 摘　　要：AIM:To investigate the anti-tumor effects of Paris chinensis dioscin(PCD)and mechanisms regarding cell cycle regulation and apoptosis in human gastric cancer SGC-7901 cells.METHODS:Cell viability was analyzed by the 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay.Cell apoptosis was evaluated by flow cytometry and laser scanning confocal microscope(LSCM)using Annexin-V/propidium iodide(PI)staining,and the cell cycle was evaluated using PI staining with flow cytom-etry.Intracellular calcium ions were detected under fluorescence microscope.The expression of cell cycle and apoptosis-related proteins cyclin B1,CDK1,cytochrome C and caspase-3 was measured by immunohistochemical staining.RESULTS:PCD had an anti-proliferation effect on human gastric cancer SGC-7901 cells in a dose-and time-de-pendent manner.After treatment of SGC-7901 cells with PCD,apoptosis appeared in SGC-7901 cells.Morpho-logical changes typical of apoptosis were also observed with LSCM by Annexin V/PI staining,and the cell number of the G0/G1 phase was decreased,while the number of cells in the G2/M phase was increased.Cell cycle-related proteins,such as cyclin B1 and CDK1,were all down-regulated,but caspase-3 and cytochrome C were up-regulated.Moreover,intracellular calcium accumulation occurred in PCD-treated cells.CONCLUSION:G2/M phase arrest and apoptosis induced by PCD are associated with the inhibition of CDK-activating kinase activity and the activation of Ca2+-related mitochondrion pathway in SGC-7901 cells. 关键词：细胞周期阻滞细胞凋亡薯蓣皂苷 SGC G2 巴黎胃癌 M期

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Paris chinensis dioscin induces G2/M cell cycle arrest and apoptosis in human gastric cancer SGC-7901 cells

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