Identification, Genetic Analysis and Mapping of Resistance to Phytophthora sojae of Pm28 in Soybean

查看全文 作　　者：WU Xiao-[1,2]ling;ZHANG Bao-[1]qiang;SUN [1]Shi;ZHAO Jin-[1]ming;YANG [2]Feng;GUO [1]Na;GAI Jun-[1]yi;XING [1]Han

机构地区：[1]Soybean Research Institute, Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R.China;[2]College of Agriculture, Sichuan Agricultural University, Ya'an 625014, P.R. China

出　　处：《Agricultural Sciences in China》索引2011年第10卷第10期,共6页

基　　金：supported by the Earmarked Fund for Modern Agro-Industry Technology Research System, China (nyhyzx07-053);the Program for Changjiang Scholars and Innovative Research Team in University, China (PCSIRT);the Research Fund for the Doctoral Program of Higher Education of China (20090097120023) 摘　　要：Phytophthora sojae Kanfman and Gerdemann (P. sojae) is one of the most prevalent pathogens and causes Phytophthora root rot, which limits soybean production worldwide. Development of resistant cultivars is a cost-effective approach to controlling this disease. In this study, 127 soybean germplasm were evaluated for their responses to Phytophthora sojae strain Pm28 using the hypocotyl inoculation technique, and 49 were found resistant to the strain. The hypocotyl of P1, P2, F1, and F2:3 of two crosses of Ludou 4 (resistant)×Youchu 4 (susceptible) and Cangdou 5 (resistant)×Williams (susceptible) were inoculated with Pm28, and were used to analyze the inheritance of resistance. The population derived from the cross of Ludou 4×Youchu 4 was used to map the resistance gene (designated as Rps9) to a linkage group. 932 pairs of SSR primers were used to detect polymorphism, and seven SSR markers were mapped near the resistance gene. The results showed that the resistance to Pm28 in Ludou 4 and Cangdou 5 was controlled by a single dominant gene Rps9, which was located on the molecular linkage group N between the SSR markers Satt631 (7.5 cM) and Sat_186 (4.3 cM). 关键词：大豆疫霉抗性遗传遗传分析 SSR标记鉴定测绘接种技术抗性基因

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Identification, Genetic Analysis and Mapping of Resistance to Phytophthora sojae of Pm28 in Soybean

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