Functional characterization of a biosurfactant-producing thermo-tolerant bacteria isolated from an oil reservoir

查看全文 作　　者：Wang [1]Jing;Ji [1]Guang;Tian [1]Jing;Zhang [1]Hongdan;Dong [1]Hanping;Yu [2]Li

机构地区：[1]College of Chemical Engineering, China University of Petroleum, Beijing 102249, China;[2]Langfang Branch, Research Institute of Petroleum Exploitation and Development, China National Petroleum Corporation, Hebei 065007, China

出　　处：《Petroleum Science》索引2011年第8卷第3期,共4页

基　　金：supported by the National High-technology Research and Development Program(863Program)of China(Grant No.2009AA063504);China National Petroleum Corporation Science&Technology Management Program(2008A-1403) 摘　　要：A Bacillus subtilis strain JA-1 isolated from an oil reservoir was studied.This strain is capable of growth and producing biosurfactant at a temperature of 60 oC.In nutrient medium it produced biosurfactant which reduced the surface tension from 68.2 mN/m to 28.3 mN/m,with the critical micelle concentration (CMC) of 48 mg/L.The measured surface tension indicated that the biosurfactant possessed stable surface activity at high temperature and a specific range of pH and salt concentrations.The results of thin layer chromatography (TLC) together with FT-IR showed that the metabolic product of strain JA-1 is a lipopeptide biosurfactant.The ability to growth at high temperature and to produce biosurfactant makes strain JA-1 promising for enhanced oil recovery. 关键词：生物表面活性剂菌株分离功能特性耐热细菌生产油藏枯草芽孢杆菌应变能力

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Functional characterization of a biosurfactant-producing thermo-tolerant bacteria isolated from an oil reservoir

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