沉默COX-2抑制A549细胞的恶性增殖(英文)

查看全文 作　　者：Weiying Li Wentao Yue Lina Zhang Xiaoting Zhao Li Ma Xuehui Yang Chunyan Zhang Yue Wang Meng [1]Gu 机构地区：[1]Department of Cell Molecular Biology, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing Chest Hospital, Beijing 101149, China 出　　处：《The Chinese-German Journal of Clinical Oncology》索引2011年第10卷第7期,共5页 摘　　要：Objective: The aim of this study was to explore the effects on malignant proliferation of A549 cell by silencing cy-clooxygenase (COX)-2. Methods: In the present study, we constructed three siRNA vectors producing small interference RNA. The siRNA vectors and the vacant vectors were transfected into A549 cell with lipofectamine respectively and the transfected cell strains were constructed. The change of COX-2 expression levels was examined by Western blot and RT-PCR. The effects on the proliferation of lung cancer cells were studied by cell growth curve, clonogenic assay and xenograft assays. Results: The siRNA expression vectors produced marked effects in A549 cell but the inhibited effects were different. The effect of psi-10 was best and the mRNA and protein levels of COX-2 reduced 61.2% and 56.2% respectively in A549-si10 cell in contrast to the control. The growth of A549 cell slowed and the colony formation rate reduced after silencing COX-2. In xenograft assays, the growth speeds of tumor became slow and the numbers of tumor reduced after silencing COX-2. Conclusion: The si10 target of COX-2 has the best silencing effect in A549 cell and the best inhibition effect on malignant proliferation of A549 cell in vivo and in vitro. 关 键 词：A549细胞 细胞增殖 COX 小分子干扰RNA 细胞生长曲线 siRNA 脂质体转染 表达载体