Application of DNA barcodes in Hedyotis L.(Spermacoceae,Rubiaceae)

查看全文 作　　者：Xing [1,2]GUO;Mark R [3]SIMMONS;Paul Pui-Hay [4]BUT;Pang-Chui [4]SHAW;Rui-Jiang [1]WANG

机构地区：[1]Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China;[2]Graduate University of the Chinese Academy of Sciences, Beijing 100049, China;[3]Department of Biology, Colorado State University, Fort Collins, Colorado 80523-1878, USA;[4]School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, China

出　　处：《Journal of Systematics and Evolution》索引2011年第49卷第3期,共10页

基　　金：supported by the Research Fund for the Large-Scale Scientific Facilities of the Chinese Academy of Sciences (grant no.2009-LSF-GBOWS-01);the Knowledge Innovation Program of the Chinese Academy of Sciences(grant no.KSCX2-EW-J-28) 摘　　要：The potential application of DNA barcodes of plastid(matK,trnH-psbA,petD,and rbcL) and nuclear(internal transcribed spacer(ITS) of rDNA) DNA regions was investigated for 25 Hedyotis taxa.The ITS showed the best species discrimination by resolving 23 of the species as exclusive lineages with no shared alleles between any of the 24 distinct species(H.assimilis and H.mellii are not supported as distinct species based on our molecular and morphological data).Conversely,rbcL performed the worst and only resolved 10 of the species as exclusive lineages,and 10 species with shared alleles.Using ITS has the advantage of high PCR amplification success and it provides good intra-and interspecific variation distribution patterns.The most powerful plastid markers were petD and trnH-psbA,but we could amplify and sequence trnH-psbA for only 83% of the accessions sampled.Combination of ITS and petD performed extremely well,with all 24 of the distinct species resolved as exclusive lineages and no shared alleles between any of the distinct species.We therefore recommend ITS,or a combination of ITS and petD,as the standard DNA barcode in Hedyotis,but acknowledge that there are no shared alleles between distinct species for matK and rbcL combined. 关键词：白花蛇舌草 RDNA 条形码应用茜草科 PSBA基因 RBCL基因等位基因

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Application of DNA barcodes in Hedyotis L.(Spermacoceae,Rubiaceae)

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