Modulation of synchronous calcium oscillations in hippocampal neurons by photostimulation of astrocytes with femtosecond laser

查看全文 作　　者：ZHAO Yuan LIU XiuLi ZHANG Yuan ZHOU Wei ZENG [1]ShaoQun

机构地区：[1]Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Tech-nology, Wuhan 430074, China

出　　处：《Chinese Science Bulletin》索引2010年第55卷第30期,共5页

基　　金：supported by the National Natural Science Foundation of China (30927001);the National Science Fund for Distinguished Young Scholars (30925013);the'111'Project 摘　　要：A large body of evidence indicates that astrocytes play an important role in a range of brain functions through calcium (Ca2+) signaling. Experimentally evoking Ca2+ signaling is a useful technique for investigating the functions of astrocytes. However,conventional stimulation methods typically have poor spatio-temporal precision,and some are invasive. Our group has developed a technique to overcome these problems,in which astrocytes are photostimulated with a femtosecond laser. In the current study,we applied this method to a hippocampal neural network to explore astrocytic functions in detail. The results revealed that applying photostimulation to astrocytes in a cultured hippocampal astrocyte-neuron network caused the following changes:(i) Syn-chronous Ca2+ oscillations in neurons were induced; (ii) spontaneous Ca2+ synchrony instantaneously emerged; and (iii) high-frequency spontaneous Ca2+ synchrony was regulated. Thus,astrocytic Ca2+ signaling evoked by photostimulation was found to modulate synchronous Ca2+ oscillations in hippocampal neurons. We propose that photostimulation with a femtosecond laser will serve as a powerful tool in investigating astrocytic functions at the network level. 关键词：星形胶质细胞海马神经元飞秒激光钙振荡同步性调制细胞功能神经网络

相关文献

参考文献（32）
引证文献（1）
耦合文献（7）

参考文献(32)

引证文献(1)

耦合文献(7)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Modulation of synchronous calcium oscillations in hippocampal neurons by photostimulation of astrocytes with femtosecond laser

参考文献(32)

引证文献(1)

耦合文献(7)

用户提示