Molecular Characterization of Nonstructural Protein NS38 of Grass Carp Reovirus

查看全文 作　　者：Ling [1,2]SHAO;Chao [1,2]FAN;Ewa [1]Maj;Qin [1]FANG

机构地区：[1]State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China;[2]Graduate School of the Chinese Academy of Sciences, Beijing 100039, China

出　　处：《Virologica Sinica》索引2010年第25卷第2期,共7页

基　　金：National Basic Research Program (973) of China ( 2009CB118701);National Natural Scientific Foundation of China (30871940, 30671615) 摘　　要：Viral nonstructural proteins in both enveloped and non-enveloped viruses play important roles in viral replication. Protein NS38 of Grass carp reovirus (GCRV), has been deduced to be a non-structural protein, and, consistent with other reoviruses, is considered to cooperate with the NS80 protein in viral particle assembly. To investigate the molecular basis of the role of NS38, a complete protein was expressed in E.coli for the first time. It was found that there is a better expression of NS38 induced with IPTG at 28 ℃ rather than 37 ℃. In addition, the antiserum of NS38 prepared with purified fusion protein and injected into rabbit could be used for detecting NS38 protein expression in GCRV infected cell lysate, while there is not any reaction crossed with purified virus particle, confirming NS38 is not a component of the viral structural protein. The result reported in this study will provide evidence for further viral protein-protein and protein-RNA interaction in dsRNA viruses replication. 关键词：草鱼呼肠孤病毒非结构蛋白分子特性蛋白质分子 RNA病毒病毒复制病毒蛋白病毒粒子

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Molecular Characterization of Nonstructural Protein NS38 of Grass Carp Reovirus

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