In vitro Anti-viral Activity of the Total Alkaloids from Tripterygium hypoglaucum against Herpes Simplex Virus Type 1

查看全文 作　　者：Zhe [1,2]REN;Chuan-hai [1,4]ZHANG;Lian-jun [1,3]WANG;Yun-xia [1,3]CUI;Ren-bin [2]QI;Chong-ren [5]YANG;Ying-jun [5]ZHANG;Xiao-yi [6]WEI;Da-xiang [2]LU;Yi-fei [1,3]WANG

机构地区：[1]Biomedicine Research and Development Center of Jinan University, Guangzhou 510632, China;[2]MedicalCollege of Jinan University, Guangzhou 510632, China;[3]National Engineering Research Center of GeneticMedicine, Guangzhou 510632, China;[4]Department of Chemistry and Biology, West Anhui University, Lu'an 237012, China;[5]Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China;[6]SouthChina Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510000, China

出　　处：《Virologica Sinica》索引2010年第25卷第2期,共8页

基　　金：The Joint Funds of National Science Foundation of China (U0632010);The State Key Laboratory of Phytochemistry and Plant Resources in West China;Chinese Academy of Sciences (O807B11211, O807E21211);'211 grant of MOE' 摘　　要：Herpes simplex virus type 1 (HSV-1) is a commonly occurring human pathogen worldwide. There is an urgent need to discover and develop new alternative agents for the management of HSV-1 infection. Tripterygium hypoglaucum (level) Hutch (Celastraceae) is a traditional Chinese medicine plant with many pharmacological activities such as anti-inflammation, anti-tumor and antifertility. The usual medicinal part is the roots which contain about a 1% yield of alkaloids. A crude total alkaloids extract was prepared from the roots of T. hypoglaucum amd its antiviral activity against HSV-1 in Vero cells was evaluated by cytopathic effect (CPE) assay, plaque reduction assay and by RT-PCR analysis. The alkaloids extract presented low cytotoxicity (CC50 = 46.6 μg/mL) and potent CPE inhibition activity, the 50% inhibitory concentration (IC50) was 6.5 μg/mL, noticeably lower than that of Acyclovir (15.4 μg /mL). Plaque formation was significantly reduced by the alkaloids extract at concentrations of 6.25 μg/mL to 12.5 μg/mL, the plaque reduction ratio reached 55% to 75% which was 35% higher than that of Acyclovir at the same concentration. RT-PCR analysis showed that, the transcription of two important delayed early genes UL30 and UL39, and a late gene US6 of HSV-1 genome all were suppressed by the alkaloids extract, the expression inhibiting efficacy compared to the control was 74.6% (UL30), 70.9% (UL39) and 62.6% (US6) respectively at the working concentration of 12.5μg/mL. The above results suggest a potent anti-HSV-1 activity of the alkaloids extract in vitro. 关键词：单纯疱疹病毒活动类型总生物碱雷公藤抗病毒海棠体外

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In vitro Anti-viral Activity of the Total Alkaloids from Tripterygium hypoglaucum against Herpes Simplex Virus Type 1

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