Influence of Hypericum perforatum Extract on Piglet Infected with Porcine Respiratory and Reproductive Syndrome Virus

查看全文 作　　者：PU Xiu-[1,3]ying;LIANG Jian-[2,3]ping;SHANG Ruo-[3]feng;WANG Xue-[3]hong;WANG Zuo-[1,3]xin;HUA Lan-[3]ying;LIU [3]Yu

机构地区：[1]College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, P.R.China;[2]Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, P.R.China;[3]Lanzhou Institute of Animal and Veterinary Pharmaceutics Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, P.R. China

出　　处：《Agricultural Sciences in China》索引2009年第8卷第6期,共10页

基　　金：supported by One Hundred Person Project of the Chinese Academy of Sciences (Renjiaozi[2008] 287);the Special Fund to Aid Basic Scientific Research of State Level Research Institutes for Public Welfare, China (BRF070402) 摘　　要：To study the influence of Hypericum perforatum extract (HPE) on piglets infected with porcine respiratory and reproductive syndrome virus (PRRSV), enzyme-labeled immunosorbent assay (ELISA) and cytopathic effect (CPE) were used to determine in vitro whether HPE could induce swine pulmonary alveolar macrophages (PAMs) to secrete IFN-γ, and whether PRRSV titers in PAMs were affected by the levels of HPE-induced IFN-γ. HPE (200 mg kg-1) was administrated by oral gavage to piglets infected with the PRRSV in vivo to observe whether HPE affected the viremia, lung viral titers, and weight gain of piglets infected with PRRSV. The results showed that HPE was capable of inducing PAMs to produce IFN-γ in a dose dependent manner and HPE pretreatment was capable of significantly reducing PRRSV viral titers in PAMs (P < 0.01). Administration of HPE to the PRRSV-infected animals significantly (P < 0.05) reduced viremia over time as compared with the PRRSV-infected animals. But there was not significant decrease in lung viral titers at day 21 post-infection between the HPE-treated animals and the PRRSV-infected control piglets. There were no significant differences in weight gain over time among the HPE-treatment animals, the normal control, and the HPE control animals. The PRRSV-infected animals caused significant (P < 0.01) growth retardation as compared with the HPE controls and the normal piglets. It suggested that HPE might be an effective novel therapeutic approach to diminish the PRRSV-induced disease in swine. 关键词：猪繁殖与呼吸综合征病毒滴度贯叶连翘提取物呼吸道仔猪感染肺泡巨噬细胞

相关文献

参考文献（14）
引证文献（11）
耦合文献（0）

参考文献(14)

引证文献(11)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Influence of Hypericum perforatum Extract on Piglet Infected with Porcine Respiratory and Reproductive Syndrome Virus

参考文献(14)

引证文献(11)

用户提示