Gene mapping of a new coat mutation in mouse

查看全文 作　　者：Shanru Li;Dongping Wang;Hong Lan;Wenyan Zhang;Baosheng Ge;Jiqing Li;Cuie Wang;Yifan Lu;Yanyan Shi;Runfang Li

机构地区：[1]Inst Jingfeng Med Lab Anim, Beijing 100071, Peoples R China;[2]Chinese Acad Sci, Kunming Inst Zool, Kunming 650107, Peoples R China;[3]Changchun Univ Agr & Anim Sci, Changchun 130062, Peoples R China;[4]Beijing Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China;[5]Beijing Inst Biotechnol, Beijing 100071, Peoples R China;

出　　处：《Chinese Science Bulletin》索引1999年第44卷第13期,共4页

摘　　要：Gene mapping of a mouse coat mutation has been investigated. First, 100 10-bp random primers were used to amplify DNA, but the mutation could not be located by this method because there were no correlation between the amplified products and coat phenotypes. Second, by using ldh1 , Car2, Mup1 , Pgb1 , Hbb, Es1O, Es1, Mod1, Gdc1 , Ce2, Es3 as genetic markers, linkage test crosses (two-point test) consisting of intercrossing uncovered BALB/c mice (homozygotes) to CBA/N and C57BL/6 mice with normal hair and backcrossing the heterozygotes of the F1 to the uncovered BALB/c mice were made. It was soon evident that the mutation was linked to Es3 on chromosome 11. Furthermore, three-point test was made by using Es3 and D11 Mit8 (a microsatellite DNA) as genetic markers. The result showed that the mutation was linked to Es3 with the percentage recombination of (7.89 ± 2.19) %, and linked to D11Mit8 with the percentage recombination of (26.30 ± 3.57)%. The percentage recombination between Es3 and D11Mit8 was 关键词：MOUSE MUTATION GENE mapping.

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Gene mapping of a new coat mutation in mouse

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