维普中文期刊产品整合服务

Con A affinity glycoproteomics of normal human liver tissue

查看全文 作　　者：SUN QiangLing1, LIU YinKun1, LU WenJing1, CHENG Gang2, ZHOU HaiJun1, ZHOU XinWen3, WEI LiMing3, DAI Zhi1, GUO Kun1 & LU HaoJie3 1 Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China;2 System Biology Lab, Institute of Biomedical Sciences, Fudan University, Shanghai 200433, China 3 Research Center for Proteomics, Institute of Biomedical Sciences, Fudan University, Shanghai 200433, China 高影响力作者 出　　处：《Science China(Life Sciences)》索引2007年第50卷第3期,共9页高影响力期刊 基　　金：the National Basic Research Program of China (973)(Grant No. 2004CB520802) 摘　　要：In order to establish the novel high throughput, high efficiency and low cost technological platform for the research of N-glycoproteomics, to resolve the significance of characteristic expression profile of glycoprotein and to find the proteins with biological functional importance, the glycoproteins with high-mannose core and the two antennary types were purified and enriched by the Con A affinity chromatography. Con A affinity protein expression profiles of normal human liver tissue were gener- ated by using SDS-PAGE, two-dimensional electrophoresis (2-DE) followed by fast fluorescence stain- ing based on multiplexed proteomics (MP) technology. 301 visible protein spots on the gel were de- tected and 85 of glycoproteins were further successfully identified via peptide mass fingerprinting (PMF) by a matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF- MS/MS) and annotated to IPI databases. Identified glycoproteins definitely take part in the regulation of cell cycle and metabolic processes. The glycosylation sites were predicted with NetNGlyc 1.0 and NetOGlyc 3.1 software, meanwhile they were classified according to the geneontology methods. The construction of Con A affinity glycoprotein database of normal human liver tissue would contribute to the subsequent research. 关 键 词：Con A affinity glycoproteomics of normal human liver tissue
相关文献

参考文献(10)

引证文献(2)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费