SCFP, a novel fiber-specific promoter in cotton

查看全文 作　　者：HOU Lei LIU Hao LI JiaBao YANG Xia XIAO YueHua LUO Ming SONG ShuiQing YANG GuangWei PEI [1]Yan

机构地区：[1]Key Laboratory of Biotechnology and Crop Quality Improvement of the Ministry of Agriculture, Biotechnology Research Center, Southwest University, Chongqing 400716, China

出　　处：《Chinese Science Bulletin》索引2008年第53卷第17期,共7页

基　　金：National Basic Research Program of China (Grant No. 2004CB117304);National Hi-Tech Research and Development Program of China (Grant No. 2007AA10Z115);the National Natural Science Foundation of China (Grant No. 30671326) 摘　　要：To investigate the expression pattern of GhSCFP which was isolated from cotton fiber cDNA library, a 1006 bp upstream fragment of the gene was cloned by chromosome walking and fused to GUS and GFP respectively. Histochemical GUS and GFP fluorescence analysis revealed that the expression of the report genes driven by the promoter sequence was detectable only in outer layer cells during the seed development in the transgentic tobaccos. In transgenic cotton, strong GUS activity was observed in spherical protrusions on 0 dpa (days post anthesis) ovule surface, and in the 2―36 dpa fiber cells, while no GUS signals were detected in the root, leaves, stem, corolla, anther and stigma. Our data demonstrated that GhSCFP upstream sequence is a cotton fiber-specific promoter and this promoter will be useful in the molecular research on fiber cell development and in cotton fiber improvements by genetic modification. 关键词：棉花纤维促进剂 SCFP 烟草植物生长调节剂

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SCFP, a novel fiber-specific promoter in cotton

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