Histopathological Observation of Lymphocystis Disease and Lymphocystis Disease Virus (LCDV) Detection in Cultured Diseased Sebastes schlegeli

查看全文 作　　者：SHENG Xiuzhen ZHAN Wenbin XU Songjuan CHENG [1]Shunfeng

机构地区：[1]Laboratory of Pathology and lmmunology of Aquatic Animals, LMMEC, Ocean University of China, Qingdao 266003, P. R. China

出　　处：《Journal of Ocean University of China》索引2007年第6卷第4期,共5页

基　　金：This work was supported by National Natural Science Foundation of China (No. 30271016);the National High Technology Development Program of China (863) (No. 2006AA100306). 摘　　要：Lymphocystis nodules occurring in the cultured sting fish Sebastes schlegeli were observed under light and electron microscope. Lymphocystis disease virus (LCDV) in the tissues of diseased fish was detected with indirect immunofluorescence test (IFAT). Results showed that lymphocystis cells had overly irregular nuclei, basophilic intracytoplasmic inclusion bodies with virions budding from the surface, and hyaline capsules outside the cell membrane. Numerous virus particles about 200 nm in diameter scat- tered in the cytoplasm, electron-dense particles 70-80 nm in diameter filled in perinuclear cisterna, and membrane-enveloped parti- cles with electron-dense core of 70-80 nm appeared around cellular nucleus. IFAT using monoclonal antibody against LCDV from Paralichthys olivaceus revealed that specific green fluorescence was present in the cytoplasm of lymphocystis cells, epithelium of stomach, gill lamellae, and muscular fibers under epidermis of S. schlegeli, just as that in the cytoplasm of lymphocystis cells of P. olivaceus, suggesting the presence of LCDV in these tissues. 关键词：组织病理学超微结构荧光免疫学刺鱼

相关文献

参考文献（11）
引证文献（6）
耦合文献（31）

参考文献(11)

引证文献(6)

耦合文献(31)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Histopathological Observation of Lymphocystis Disease and Lymphocystis Disease Virus (LCDV) Detection in Cultured Diseased Sebastes schlegeli

参考文献(11)

引证文献(6)

耦合文献(31)

用户提示