Inhibition of Differentiation by Transforming Growth Factor β1 in Rhabdomyosarcoma Cells

查看全文 作　　者：Shouli [1]Wang;Huihua [2]Yao;Zhenghong [3]Qin;Shigang [1]Li;Yizhong [1]Feng;Xiuzhen [1]Wang;Min [1]Deng;Lingling [1]Guo;Lifeng [1]Zhang

机构地区：[1]Molecular Pathology Laboratory, Medical School of Soochow University, Suzhou 215123, China;[2]Department of Surgery, the Second Affiliated Hospital of Soochow University, Suzhou 215123, China.;[3]Pharmacology Laboratory, Medical School of Soochow University, Suzhou 215123, China

出　　处：《Chinese Journal of Clinical Oncology》索引2007年第4卷第5期,共6页

基　　金：the grants fromthe Jiangshu Provincial Higher InstitutionNatural Science Foundation(No.2134605);Soochow Universtiy Medicine DevelopmentFoundation(No.EE134513);the Ji-angshu Provincial Post-Doctoral Foundation(No.51208). 摘　　要：OBJECTIVE To study the effect of transforming growth factor?1 (TGF-β1)on differentiation of rhabdomyosarcoma(RMS)cells METHODS RD(human embryonal RMS cell line)cells,cultured in differentiation medium containing 9-cis retinoic acid(9CRA),were treated with TGF-β1.Proliferation of the cells was examined by the MTT assay.The differentiation specific proteins(sarcomeric actin and MyHC)and myogenic transcription factors(MyoD1 and myogenin)in the RD cel s were assessed by immunofluorescence staining. RESULTS Compared to control cel s,treatment with lower concentrations of TGF-?1(0.1 and 0.2 ng/ml)induced an increase in OD values after 4 d(P<0.01),whereas higher concentrations of TGF-?1(2 and 5 ng/ml)led to a reduction of cell viability(P<0.01).After exposure to 9CRA,the viability of the cells decreased significantly(P<0.01),while sarcomeric actin,MyHC and myogenin were induced.These changes were antagonized by TGF-β1(0.1 ng/ml).No changes were observed in expression of MyoD1. CONCLUSION The RMS cells,derived from myogenic progenitors are committed to a myogenic fate,but are arrested in the differentiation course by the addition of TGF-?1 which represses some of the myogenic transcription factors. 关键词：横纹肌肉瘤癌症治疗方法转变模式

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Inhibition of Differentiation by Transforming Growth Factor β1 in Rhabdomyosarcoma Cells

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