Study on ELISA for detection of sulfamethazine residues

查看全文 作　　者：LI Jichang HE Liang XU Shiwen HUO Guicheng DING Liangjun LIU [1]Ning

机构地区：[1]College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China

出　　处：《Journal of Northeast Agricultural University(English Edition)》索引2007年第14卷第3期,共5页

基　　金：Supported by Key Items of National Technology Research Project(2002BA518A06);Heilongjiang Provincial Science and Technology Tackle Key Problem Project(10541021);Harbin Technical Fund(2003AA6CN179) 摘　　要：In this study,various factors of ELISA for detection of sulfamethazine residues were explored,the coating antigen was diluted to 1:400,the best coating condition was at 4℃overnight,the working concentration of HRP-IgG enzyme conjugate was 1:7 000.The pre-incubation time and incubation time was 30 min and 120 min,respectively,the substrate solution working time was 20 min.Two moL·L^(-1) H_2SO_4 was used to stop the reaction and checked.A standard curve of direct competitive ELISA had been established to detect the sulfamethazine residues in milk.The detection limit of this method was 1.97 ng·mL^(-1).The mean concentration of sulfamethazine required to inhibit 30% antibody was 7.1 ng·mL^(-1).The linear range of the detection was 5-200 ng·mL^(-1). The recovery ratio was between 73.20% and 91.16%.The CV% of within array and between arrays was less than 10%. 关键词：磺胺甲嘧啶单克隆抗体药物残留检测 ELISA

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Study on ELISA for detection of sulfamethazine residues

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