Identification and primary genetic analysis of Arabidopsis stomatal mutants in response to multiple stresses

查看全文 作　　者：SONG Yuwei KANG Yanli LIU Hao ZHAO Xiaoliang WANG Pengtao AN Guoyong ZHOU Yun MIAO Chen SONG [1]Chunpeng 机构地区：[1]Laboratory of Plant Stress Biology, Department of Biology, Henan University, Kaifeng 475001, China 出　　处：《Chinese Science Bulletin》索引2006年第51卷第21期,共9页 基　　金：This work was supported by the National Key Basic Special Funds(Grant No.2003CB114305);the National Natural Science Foundation of China(Grant Nos.39870372,30370765 and 30440079). 摘　　要：In response to variable environmental conditions, guard cells located in the leaf epidermis can integrate and cope with a multitude of complicated stimuli, thereby making stomata in an appro- priate state. However, many signaling components in guard cell signaling remain elusive. In our laboratory, a tool for non-invasive remote infrared thermal images was used to screen an ethyl methane sulfonate-mutagenized population for Arabidopsis stomatal response mutants under multiple stresses (ABA, H2O2, CO2, etc.). More than forty 'hot' or 'cold' mutants were isolated (above or below 0.5℃ in con- trast to normal plantlets). Identification and primary genetic analysis of these mutants show that they are monogenic recessive mutations and there exist distinct difference in stomata apertures compared to wild type. These mutants in response to various environmental stresses and hormones were comprehen- sively investigated, which enables us to further un- derstand the cross-talk in different signal transduction pathways. 关 键 词：红外摄象机 保卫细胞 遗传分析 信号转导变异