Synthesis and Identification of Artificial Antigen for Imidacloprid

查看全文 作　　者：ZHU Guo-[1]nian;GUI Wen-[1]jun;ZHENG Zun-[1]tao;CHENG Jing-[1]li;WU [2]Gang 机构地区：[1]Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, P.R.China;[2]Zhejiang Entry-Exit Inspection and Quarantine Bureau, Hangzhou 310012, P.R.China 出　　处：《Agricultural Sciences in China》索引2006年第5卷第4期,共6页 基　　金：the National Natural Science Foundation of China (30370944). 摘　　要：This study reported that a hapten of imidacloprid, 1-[(6-Carboxylethylthio-3- pyridinyl)methyl]-N -nitro-imidazolidinimine was synthesized using technical material of imidacloprid to react with 3-mercaptopropionic acid in hot alkaline solution. The hapten was conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to form the immuno-antigens and the coating antigen, respectively. The antibody with high titer (2.56 × 104) was obtained after immuning rabbits. The results showed that the antibody had a high affinity to imidacloprid tested by enzyme-linked immunosorbent assay (ELISA), which IC50 and IC20 were 20.7 and 1.1 μg L-1; and the cross reactibilities to those compounds related with imidacloprid were less than 1.4%. 关 键 词：抗体 人造抗原 土壤 种子 碱性条件