Apoptosis mechanisms of human gastric cancer cell line MKN-45 infected with human mutant p27

查看全文 作　　者：Jin-Shui Zhu Long Wang Guo-Qiang Cheng Qin Li Zu-Ming Zhu Li [1]Zhu

机构地区：[1]Department of Gastroenterology, Affiliated Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China

出　　处：《World Journal of Gastroenterology》索引2005年第11卷第47期,共5页

基　　金：Supported by the Natural Science Foundation of Shanghai,No. 04ZB14072 摘　　要：AIM: To explore the inducing effect of human mutant p27 gene on the apoptosis of the human gastric cancer cell line MKN-45 and its associated mechanisms. METHODS: The recombinant adenovirus Ad-p27mt was constructed to infect the human gastric cancer cell line MKN-45. Using flow cytometry, TUNEL assay and DNA fragment analysis, we measured the apoptotic effect of Ad-p27mt on the human gastric cancer cells. RESULTS: Ad-p27mt was successfully constructed and the infection efficiency reached 100%. After 18 h of infection, we observed an apoptotic hypodiploid peak on the flow cytometer before G1-S and apoptotic characteristic bands in the DNA electrophoresis. The apoptotic rate detected by TUNEL method was significantly higher in the Ad-p27mt group (89.4±3.12%)compared to the control group (3.12±0.13%, P < 0.01).CONCLUSION: Human mutant p27 can induce apoptosis of the human gastric cancer cells in vitro. 关键词：细胞凋亡 MKN-45 胃癌肿瘤细胞

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Apoptosis mechanisms of human gastric cancer cell line MKN-45 infected with human mutant p27

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