Study on the adoption of Schwann Cell Phenotype by Bone Marrow Stromal Cells in vitro and in vivo

查看全文 作　　者：FU-QIANG ZHAO PEI-XUN ZHANG XIANG-JUN HE CHAN DU ZHONG-GUO FU DIAN-YING ZHANG BAO-GUO [1]JIANG

机构地区：[1]People's Hospital, Peking University, Beijing 100044, China

出　　处：《Biomedical and Environmental Sciences》索引2005年第18卷第5期,共8页

基　　金：This research was supported by the 863 Program (2002AA205071) and National Natural Foundation of China (30271306) 摘　　要：Objective To explore the possibilities of bone marrow stromal cells (MSCs) to adopt Schwann cell phenotype in vitro and in vivo in SD rats. Methods MSCs were obtained from tibia and femur bone marrow and cultured in culture flasks. Beta-mercaptoethanol followed by retinoic acid, forskolin, basic-FGF, PDGF and heregulin were added to induce differentiation of MSCs’. Schwann cell markers, p75, S-100 and GFAP were used to discriminate induced properties of MSCs’ by immunofluorescent staining. PKH-67-labelled MSCs were transplanted into the mechanically injured rat sciatic nerve, and laser confocal microscopy was performed to localize the PKH67 labelled MSCs in the injured sciatic nerve two weeks after the operation. Fluorescence PKH67 attenuation rule was evaluated by flow cytometry in vitro. Results MSCs changed morphologically into cells resembling primary cultured Schwann cells after their induction in vitro. In vivo, a large number of MSCs were cumulated within the layer of epineurium around the injured nerve and expressed Schwann cell markers, p75, S-100, and GFAP. Conclusion MSCs are able to support nerve fiber regeneration and re-myelination by taking on Schwann cell function, and can be potentially used as possible substitutable cells for artificial nerve conduits to promote nerve regeneration. 关键词：施沃恩细胞骨髓干细胞人体细胞萤光免疫检验法机械损伤

相关文献

参考文献（21）
引证文献（4）
耦合文献（0）

参考文献(21)

引证文献(4)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Study on the adoption of Schwann Cell Phenotype by Bone Marrow Stromal Cells in vitro and in vivo

参考文献(21)

引证文献(4)

用户提示