Cloning and sequence analysis of the partial sequence of the rbcL from Bryopsis hypnoides

查看全文 作　　者：TIAN [1,2]Chao;WANG [1]Guangce;YE [1,2,3]Naihao;ZHANG [1,2]Baoyu;FAN [1,2]Xiaolei;ZENG [1]Chengkui

机构地区：[1]Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;[2]Graduate School of the Chinese Academy of Sciences, Beijing 100039, China;[3]Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China

出　　处：《Acta Oceanologica Sinica》索引2005年第24卷第5期,共12页

基　　金：This study was supported by the National Natural Science Foundation of China under contract Nos 39890390,30170499,40476059 and 30250003;the Project of Scientific Innovation of Chinese Academy of Sciences under contract No.KZCX2-211;the Project of Scientific Innovation of Institute of Oceanology,Chinese Academy of Sciences under contract No.2002-2005;the“863”Project of China under contract No.2004AA603220. 摘　　要：The partial sequence of the rbcL from Bryopsis hypnoides, including the sequences of the upstream, extron and partial intron, was amplified by PCR and their sequences were determined. With Spinacia oleracea as the outgroup, neighbor-joining method and maximum parsimony method were used respectively to build phylogenetic trees according to the rbcL exon sequence among 13 species that were the typical species of six phyla. Two kinds of trees showed clearly that there were two groups among those species, the green lineage and the non-green lineage. And the relationships of algae in the green lineage were similar in the two trees but those in the non-green lineage were not consistent. Analysis of codon preference indicated that the codon preference of the rbcL exon of Bryopsis hypnoides distinctly differed from that of the relevant sequence of photosynthetic bacteria. 关键词：碳酸酵素加氧酶 PCR 克隆技术基因序列光合作用水生植物

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Cloning and sequence analysis of the partial sequence of the rbcL from Bryopsis hypnoides

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