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Interplay between nitric oxide and VIP in CCK-8-induced phasic contractile activity in the rabbit sphincter of Oddi

查看全文 作　　者：Attila Pálv(o|¨)[1]lgyi;Réka [1]Sári;József [2]Németh;Annamária [3]Szabolcs;István [3]Nagy;Péter [3]Hegyi;János [3]Lonovics;Zoltán [4]Szilvássy 高影响力作者 机构地区：[1]First Department of Medicine Faculty of Medicine,University of Szeged,H-6720 Szeged,Korányi fasor 8-10, Hungary;[2]Department of Pharmacology Medical University of Pécs, H-7643 Pécs, Szigeti u. 12, Hungary;[3]First Department of Medicine Faculty of Medicine, University of Szeged, H-6720 Szeged, Korányi fasor 8-10, Hungary;[4]Department of Pharmacology Medical University of Debrecen,H-4032 Debrecen,Nagyerdei krt.98,Hungary高影响力机构 出　　处：《World Journal of Gastroenterology》索引2005年第11卷第21期,共3页高影响力期刊 基　　金：Supported by The Wellcome Trust (Grant No. 022618),by the Hungarian Scientific Research Fund (D42188, T43066 and T042589)and by the GVOP-3.2.2.-2004-07-0001/3.0 摘　　要：AIM: The sphincter of Oddi (SO) plays an important role in delivery of bile into the duodenum. To establish whether vasoactive intestinal polypeptide (VIP) and nitric oxide (NO) were involved in phasic contractile activity of the rabbit SO stimulated by cholecystokinin-octapeptide (CCK-8).METHODS: Isolated SO muscle rings were cleaned of fat and mounted horizontally on two small L-shaped hooksone of which was connected to a force transducer for the measurement of isometric tension. The experiments were carried out in a thermostatically controlled (37±0.2℃)organ bath (5 mL) containing Krebs solution. The organ fluid was gassed with 95% O2 and 50 mL/L CO2 to keep the pH at 7.40±0.05. Contractile responses to CCK-8 (1μmol/L) were evaluated in the presence and absence of N^G-nitro-L-arginine (LNNA), an inhibitor of NO synthase (100μmol/L), and (p-chloro-D-Phe^6-Leu^17)-VIP (VIPa,30μmol/L), a VIP receptor antagonist.RESULTS: CCK-8 stimulated the phasic activity of the SO.NO synthase inhibition increased the frequency and amplitude of contractions with a slight increase in developed tension.Pre-incubation with VIPa also attenuated this CCK-8 effect.The combined application of LNNA and VIPa abolished the phasic activity of the muscle rings with a marked increase in tension in response to CCK-8.CONCLUSION: VIP and NO together contribute to an increase in phasic activity of SO. 关 键 词：一氧化氮 肠促胰酶肽-8 酶活性 兔 动物实验 括约肌 胆汁 十二指肠
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