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Genetic analysis and mapping of rice(Oryza sativa L.)male-sterile(OsMS-L)mutant

查看全文 作　　者：[1]LIUHaisheng;[1]CHUHuangwei;[1]LIHui;[1]WANGHonamei;[2]WEIJiali;[1]LINa;[1]DINGShuyan;[2]HUANGHai;[3]MAHong;[2]HUANGChaofeng;[1]LUODa;[2]YUANGzheng;[1]LIUJianhua;[5]ZHANGDabing 高影响力作者 机构地区：[1]CollegeofLifeScienceandBiotechnology,ShanghaiJiaoTongUniversity,Shanghai200240,China;[2]ShanghaiInstituteofPlantPhysiologyandEcology,ShanghaiInstituteforBiologicalSciences,ChineseAcademyofSciences,Shanghai200030,China;[3]DepartmentofBiology,HuckInstituteofLifeSciences,PennsylvaniaStateUniversity,UniversityPark,PA16802,USA;[5]CollegeofLifeScienceandBiotechnology,ShanghaiJiaoTongUniversity,Shanghai200240,China//Agro-biotechResearchCenter,ShanghaiAcademyofAgriculturalSciences,2901BeidiRoad,Shanghai201106,China高影响力机构 出　　处：《Chinese Science Bulletin》索引2005年第50卷第2期,共4页高影响力期刊 基　　金：This work Was supported by The National Basic Research Program of China(Grant No.TG1999011605);the Shanghai Municipal Committee of Science and Technology(Grant No.03DJ14016). 摘　　要：A rice male-sterile mutant OsMS-L of japonica cultivar 9522 background, was obtained in M4 population treated with Co γ-Ray. Genetic analysis indicated that the 60 male-sterile phenotype was controlled by a single recessive gene. Results of tissue section showed that at microspore stage, OsMS-L tapetum was retarded. Then tapetal cells ex- panded and microspores degenerated. No matured pollens were observed in OsMS-L anther locus. To map OsMS-L lo- cus, an F2 population was constructed from the cross be- tween the OsMS-L (japonica) and LongTeFu B(indica). Firstly, the OsMS-L locus was roughly mapped between two SSR markers, RM109 and RM7562 on chromosome 2. And then eleven polymorphic markers were developed for further fine fine-mapping. At last the OsMS-L locus was mapped between the two InDel markers, Lhs10 and Lhs6 with genetic distance of 0.4 cM, respectively. The region was delimited to 133 kb. All these results were useful for further cloning and functional analysis of OsMS-L. 关 键 词：遗传分析 稻 OsMS-L 突变异种 育种 基因图 分子标记 转基因作物
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