Pathogenicty and immune prophylaxis of cag pathogenicity island gene knockout homogenic mutants

查看全文 作　　者：Huan-JianLin JingXue YangBai Ji-DeWang Ya-LiZhang Dian-[1]YuanZhou

机构地区：[1]PLAInstituteofDigestiveMedicine,NanFangHospital,theFirstMilitaryMedicalUniversity,Guangzhou510515,GuangdongProvince,China

出　　处：《World Journal of Gastroenterology》索引2004年第10卷第22期,共3页

摘　　要：AIM: To clarify the role of cag pathogenicity island (cagPAI) of Helicobacter pylori (H pylori) in the pathogenicity and immune prophylaxis of H pylori infection. METHODS: Three pairs of H pylori including 3 strains of cagPAI positive wildtype bacteria and their cagPAI knockout homogenic mutants were utilized. H pylori binding to the gastric epithelial cells was analyzed by flow cytometry assays. Apoptosis of gastric epithelial cells induced by H pylori was determined by ELISA assay. Prophylaxis effect of the wildtype and mutant strains was compared by immunization with the sonicate of the bacteria into mice model. RESULTS: No difference was found in the apoptasis between cagPAI positive and knockout H pylori strains in respective of the ability in the binding to gastric epithelial cells as well as the induction of apoptosis. Both types of the bacteria were able to protect the mice from the infection of H pylori after immunization, with no difference between them regarding to the protection rate as well as the stimulation of the proliferation of splenocytes of the mice. CONCLUSION: The role of cagPAI in the pathogenicity and prophylaxis of Hpyloriinfection remains to be cleared. 关键词：致病性免疫学预防作用病原性脑岛基因同基因突变异种 H幽门菌

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Pathogenicty and immune prophylaxis of cag pathogenicity island gene knockout homogenic mutants

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