Anti-inflammatory effects of Mangifera indica L.extract in a model of colitis

查看全文 作　　者：Lucía [1,2]Márquez;Beatriz G Pérez-[1,3]Nievas;Icíar [1,3]Gárate;Borja García-[1,3]Bueno;José LM [1,3]Madrigal;Luis [1,4]Menchén;Gabino [5]Garrido;Juan C [1,3]Leza

机构地区：[1]Department of Pharmacology,School of Medicine,University Complutense and Centro de Investigación en Red de Salud Mental(CIBERSAM);[2]Pharmaceutical Chemistry Center and University of La Habana;[3]Health Research Institute,Hospital 12 de Octubre(I+12);[4]Gastroenterology Service,Hospital General Universitario Gregorio Maraón and CIBEREHD;[5]Department of Chemistry and Pharmacy,University Católica del Norte

出　　处：《World Journal of Gastroenterology》索引2010年第16卷第39期,共10页

基　　金：Supported by Spanish Ministry of Education(MEC,SAF0763138);the Instituto de Salud Carlos Ⅲ,Mental Health Research Network,CIBERSAM,and Foundation Santander-UCM(GR58/08) 摘　　要：AIM:To investigate the effect of aqueous extract from Mangifera indica L.(MIE)on dextran sulfate sodium (DSS)-induced colitis in rats.METHODS:MIE(150 mg/kg)was administered in two different protocols:(1)rectally,over 7 d at the same time as DSS administration;and(2)once daily over 14 d (by oral gavage,7 d before starting DSS,and rectally for 7 d during DSS administration).General observations of clinical signs were performed.Anti-inflammatory activity of MIE was assessed by myeloperoxidase(MPO)activity. Colonic lipid peroxidation was determined by measuring the levels of thiobarbituric acid reactive substances (TBARS).Reduced glutathione(GSH)levels,expression of inflammatory related mediators[inducible isoforms of nitric oxide synthase(iNOS)and cyclooxygenase (COX)-2,respectively]and cytokines[tumor necrosis factor(TNF)-αand TNF receptors 1 and 2]in colonic tissue were also assessed.Interleukin(IL)-6 and TNF-α serum levels were also measured. RESULTS:The results demonstrated that MIE has anti-inflammatory properties by improvement of clinical signs,reduction of ulceration and reduced MPO activity when administered before DSS.In addition,administration of MIE for 14 d resulted in an increase in GSH and reduction of TBARS levels and iNOS,COX-2, TNF-αand TNF R-2 expression in colonic tissue,and a decrease in IL-6 and TNF-αserum levels. CONCLUSION:MIE has anti-inflammatory activity in a DSS-induced rat colitis model and preventive administration(prior to DSS)seems to be a more effective protocol. 关键词：Oxidative stress ULCERATIVE COLITIS Inflammation POLYPHENOLS MANGIFERA INDICA ANTIOXIDANTS

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Anti-inflammatory effects of Mangifera indica L.extract in a model of colitis

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