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| 1 | MiR-375 frequently downregulated in gastric cancer inhibits cell proliferation by targeting JAK2显示文摘新兴的证据与肿瘤开发和前进显示出异常地表示的 microRNAs (miRNAs ) 的协会。然而,很少在胃的 carcinogenesis 对 miRNAs 的潜在的角色被知道。这里,我们执行了 miRNA microarray 屏蔽在配对的胃的癌症和他们的邻近的 nontumor 纸巾表示并且发现 miR-375 是的 miRNAs 差别极大地在胃的癌症纸巾的 downregulated。量的即时 PCR 分析证实那 miR-375 表情显著地从经历胃的切除术的病人与他们的 nontumor 对应物相比在超过 90% 主要胃的癌症被减少。miR-375 的 Overexpression 显著地在 vitro 并且在 vivo 禁止了胃的癌症房间增长。在胃的癌症房间的 miR-375 的强迫的表示显著地减少了 Janus kinase 的蛋白质水平 2 ( JAK2 )并且镇压带 JAK2 的 3 鈥? untranslated 区域的一个酶记者的活动,被预言的 miR-375-binding 地点的变化废除,显示那 JAK2 可以是 miR-375 目标基因。由由 RNAi 的 JAK2 的 AG490 或 silencing 的 JAK2 活动的任何一个抑制压制了类似于 miR-375 overexpression 的胃的癌症房间增长。而且, JAK2 的宫外的表示能部分颠倒 miR-375 引起的房间增长的抑制。最后,我们在胃的癌症发现了在 miR-375 表示和 JAK2 蛋白质水平之间的重要反的关联。因此,这些数据建议 miR-375 可以作为肿瘤 suppressor 工作由指向 JAK2 oncogene 潜在地调整胃的癌症房间增长,含有在胃的癌症的致病的 miR-375 的一个角色。 | Ling Ding Yanjun Xu Wei Zhang Yujie Deng Misi Si Ying Du Haomi Yao Xuyan Liu Yuehai Ke Jianmin Si Tianhua Zhou | 2010 | Cell Research2010，20，7： | 59 |
| 2 | Obesity-related glomerulopathy: pathogenesis, pathologic, clinical characteristics and treatment显示文摘 | Tianhua Xu Zitong Sheng Li Yao | 2017 | Frontiers of Medicine2017，11，3： | 23 |
| 3 | A high-quality chromosome-level genome assembly reveals genetics for important traits in eggplant显示文摘Eggplant(Solanum melongena L.)is an economically important vegetable crop in the Solanaceae family,with extensive diversity among landraces and close relatives.Here,we report a high-quality reference genome for the eggplant inbred line HQ-1315(S.melongena-HQ)using a combination of Illumina,Nanopore and 10X genomics sequencing technologies and Hi-C technology for genome assembly.The assembled genome has a total size of~1.17 Gb and 12 chromosomes,with a contig N50 of 5.26 Mb,consisting of 36,582 protein-coding genes.Repetitive sequences comprise 70.09%(811.14 Mb)of the eggplant genome,most of which are long terminal repeat(LTR)retrotransposons(65.80%),followed by long interspersed nuclear elements(LINEs,1.54%)and DNA transposons(0.85%).The S.melongena-HQ eggplant genome carries a total of 563 accession-specific gene families containing 1009 genes.In total,73 expanded gene families(892 genes)and 34 contraction gene families(114 genes)were functionally annotated.Comparative analysis of different eggplant genomes identified three types of variations,including singlenucleotide polymorphisms(SNPs),insertions/deletions(indels)and structural variants(SVs).Asymmetric SV accumulation was found in potential regulatory regions of protein-coding genes among the different eggplant genomes.Furthermore,we performed QTL-seq for eggplant fruit length using the S.melongena-HQ reference genome and detected a QTL interval of 71.29–78.26 Mb on chromosome E03.The gene Smechr0301963,which belongs to the SUN gene family,is predicted to be a key candidate gene for eggplant fruit length regulation.Moreover,we anchored a total of 210 linkage markers associated with 71 traits to the eggplant chromosomes and finally obtained 26 QTL hotspots.The eggplant HQ-1315 genome assembly can be accessed at http://gffzz5f267e24f0a44240h6pnucqcbvn5c60cc.ffgz.tsg.suse.edu.cn conclusion,the eggplant genome presented herein provides a global view of genomic divergence at the whole-genome level and powerful tools for the identification of candidate genes for important traits in eggplant. | Qingzhen Wei Jinglei Wang Wuhong Wang Tianhua Hu Haijiao Hu Chonglai Bao | 2020 | Horticulture Research2020，7，1： | 12 |
| 4 | Down-Regulation of CD44 Contributes to the Differentiation of HL-60 Cells Induced by ATRA or HMBA显示文摘CD44 is highly expressed in human acute myeloid leukemia (AML) cells. Some experiments had shown that it was possible to reverse differentiation blockage in AML cells by CD44 ligation with specific antibodies, indicating that CD44 was closely related to the differentiation of leukemia cells. The differentiation of acute promyelocytic leukemia cell line HL-60 cells could be induced by all trans-retinoic acid (ATRA) and hexamethylene bisacetamide (HMBA), but so far the mechanism was not demonstrated clearly. In the present study, we investigated whether ATRA or HMBA induced the growth arrest of HL-60 cells by down-regulating the expression of CD44. The results indicated that the proliferation of HL-60 cells was obviously inhibited and the differentiation was induced by both ATRA and HMBA. The decreased expression of CD44 and cyclin E mRNA, and the increased expression of p27 and p21 at mRNA levels were observed. Furthermore, there was a negative correlation between the expression of CD44 and p27. It was concluded that ATRA and HMBA played a role in the differentiation induction of HL-60 cells, which was mediated by the down-regulation of CD44, accompanied by down-regulation of cyclin E, and up-regulation of p27 and p21 mRNA. | Jianing Liu1, Gaofeng Bi1, Pei’e Wen1, Weihua Yang1, Xia Ren1, Tianhua Tang1, Chao Xie1, Wei Dong1 and Guosheng Jiang1, 21Department of Hemato-oncology, Institute of Basic Medicine, Shandong Academy of Medical Sciences Key Laboratory for Modern Medicine and Technology of Shandong Province Key Medical Laboratory for Tumor Immunology & Traditional Chinese Medicine Immunology of Shandong Province, Jinan, Shandong 250062, China | 2007 | Cellular & Molecular Immunology2007，4，1： | 12 |
| 5 | Twa1/Gid8 is a β-catenin nuclear retention factor in Wnt signaling and colorectal tumorigenesis显示文摘 | Yi Lu Shanshan Xie Wen Zhang Cheng Zhang Cheng Gao Qiang Sun Yuqi Cai Zhangqi Xu Min Xiao Yanjun Xu Xiao Huang Ximei Wu Wei Liu Fudi Wang Yibin Kang Tianhua Zhou | 2017 | Cell Research2017，27，12： | 9 |
| 6 | Dependability analysis of the data communication system in train control system显示文摘Communication based train control (CBTC) system is based on mobile communication and overcomes fixed blocks in order to increase track utilization and train safety. The data communication system (DCS) between trains and wayside equipment is a crucial factor for the safe and efficient operation of CBTC system. The dependability under various transmission conditions needs to be modeled and evaluated. In this paper,a stochastic reward net (SRN) model for DCS based IEEE 802.11 standard was developed,which captures all relevant failure and failure recovery behavior system aspects in a concise way. We compared the reliability,availability for DCS with and without access point (AP) and antenna redundant configuration. We also quantitatively evaluated and compared the frame loss probability for three DCS configurations with different train velocities and train numbers in one radio cell. Fixed-point iteration was adopted to simplify the analysis. Numerical results showed the significant improvement of the reliability,availability and the frame loss probability index for the full redundant configuration. | XU TianHua TANG Tao GAO ChunHai CAI BaiGen | 2009 | Science China(Technological Sciences)2009，52，9： | 7 |
| 7 | Centrosomal protein FOR20 is essential for S-phase progression by recruiting Plkl to centrosomes显示文摘中心体主要被安排微导管动力学并且便于 G1/S 和 G2/M 转变为有效房间周期前进要求。然而,在 S 阶段前进的中心体的角色大部分是未知的。这里,我们报导 FOR20 (20 kDa 的蠢人相关的蛋白质) 的那弄空,保存 centrosomal 蛋白质,禁止 S 阶段前进并且阻止 Plk1 指向(像马球的 kinase 1 ) 到中心体,在 FOR20 与 Plk1 交往的地方。Plk1 的脱离显著地也导致 S 阶段缺点,它被没能本地化到中心体的 Plk1,甚至 kinase 死了的异种,然而并非异种的宫外的表示颠倒。拴住中心体的 Plk1 的外长的表示,然而并非野类型的 Plk1,撤销 FOR20 导致弄空的 S 阶段独立于它的 kinase 活动背叛。因此,这些数据显示到由 FOR20 的中心体的 Plk1 的招募可以充当一个信号准许 S 阶段的有效前进。这代表一迄今在房间周期规定的中心体的 uncharacterized 角色。 | Minhong Shen Yuqi Cai YuehongYang Xiaoyi Yan Xiaoqi Liu Tianhua Zhou | 2013 | Cell Research2013，23，11： | 6 |
| 8 | A phosphatidic acid-binding lncRNA SNHG9 facilitates LATS1 liquid–liquid phase separation to promote oncogenic YAP signaling显示文摘Long noncoding RNAs(lncRNAs)are emerging as a new class of important regulators of signal transduction in tissue homeostasis and cancer development.Liquid–liquid phase separation(LLPS)occurs in a wide range of biological processes,while its role in signal transduction remains largely undeciphered.In this study,we uncovered a lipid-associated lncRNA,small nucleolar RNA host gene 9(SNHG9)as a tumor-promoting lncRNA driving liquid droplet formation of Large Tumor Suppressor Kinase 1(LATS1)and inhibiting the Hippo pathway.Mechanistically,SNHG9 and its associated phosphatidic acids(PA)interact with the C-terminal domain of LATS1,promoting LATS1 phase separation and inhibiting LATS1-mediated YAP phosphorylation.Loss of SNHG9 suppresses xenograft breast tumor growth.Clinically,expression of SNHG9 positively correlates with YAP activity and breast cancer progression.Taken together,our results uncover a novel regulatory role of a tumor-promoting lncRNA(i.e.,SNHG9)in signal transduction and cancer development by facilitating the LLPS of a signaling kinase(i.e.,LATS1). | Rui-Hua Li Tian Tian Qi-Wei Ge Xin-Yu He Cheng-Yu Shi Jun-Hong Li Zhen Zhang Fang-Zhou Liu Ling-Jie Sang Zuo-Zhen Yang Ya-Zhuo Liu Yan Xiong Qingfeng Yan Xu Li Huai-Qiang Ju Jian Liu Liang-Jing Wang Jian-Zhong Shao Wenqi Wang Tianhua Zhou Aifu Lin | 2021 | Cell Research2021，31，10： | 6 |
| 9 | Methane dehydroaromatization with periodic CH_4-H_2 switch:A promising process for aromatics and hydrogen显示文摘Long-term stability test of Mo/HZSM-5-N catalysts(HZSM-5-N stands for nano-sized HZSM-5) in methane dehydroaromatization(MDA)reaction has been performed with periodic CH4-H2 switch at 1033-1073 K for more than 1000 h.During this test,methane conversion ranges from 13% to 16%,and mean yield to aromatics(i.e.benzene and naphthalene) exceeds 10%.N2-physisorption,XRD,NMR and TPO measurements were performed for the used Mo/HZSM-5 catalysts and coke deposition,and the results revealed that the periodic hydrogenation can effectively suppress coke deposition by removing the inert aromatic-type coke,thus ensuring Mo/HZSM-5 partly maintained its activity even in the presence of large amount of coke deposition.The effect of zeolite particle size on the catalytic activity was also explored,and the results showed that the nano-sized zeolite with low diffusion resistance performed better.It is recognized that the size effect was enhanced by reaction time,and it became more remarkable in a long-term MDA reaction even at a low space velocity. | Changyong Sun Guangzong Fang Xiaoguang Guo Yuanli Hu Shuqi Ma Tianhua Yang Jie Han Hao Ma Dali Tan Xinhe Bao | 2015 | Journal of Energy Chemistry2015，24，3： | 6 |
| 10 | Formal verification of safety protocol in train control system显示文摘In order to satisfy the safety-critical requirements,the train control system(TCS) often employs a layered safety communication protocol to provide reliable services.However,both description and verification of the safety protocols may be formidable due to the system complexity.In this paper,interface automata(IA) are used to describe the safety service interface behaviors of safety communication protocol.A formal verification method is proposed to describe the safety communication protocols using IA and translate IA model into PROMELA model so that the protocols can be verified by the model checker SPIN.A case study of using this method to describe and verify a safety communication protocol is included.The verification results illustrate that the proposed method is effective to describe the safety protocols and verify deadlocks,livelocks and several mandatory consistency properties.A prototype of safety protocols is also developed based on the presented formally verifying method. | ZHANG Yan TANG Tao LI KePing MERA Jose Manuel ZHU Li ZHAO Lin XU TianHua | 2011 | Science China(Technological Sciences)2011，54，11： | 6 |
| 11 | Phase demodulation method based on a dual-identical-chirped-pulse and weak fiber Bragg gratings for quasi-distributed acoustic sensing显示文摘A phase demodulation method for quasi-distributed acoustic sensing(DAS)systems based on a dual-identicalchirped-pulse and weak fiber Bragg gratings(WFBGs)is proposed.Compared to the use of Rayleigh backscattering light in optical fibers,the implementation of WFBGs can contribute to obtaining an optical signal with a higher signal-to-noise ratio(SNR).The dual-identical-chirped-pulse is generated by a time-delay fiber,and the sinusoidal carrier is generated by the interference between the two chirped pulses reflected by adjacent WFBGs.The phase of the sinusoidal carrier represents the dynamic strain change posed on the sensing fiber.Discrete Fourier transform is used to directly retrieve the phase information.The performance of the phase demodulation from interference signals under different sinusoidal carrier frequencies and SNRs is numerically investigated.The piezoelectric transducer is employed to emulate the sound in the experiment to verify the effectiveness of our method.It is shown that the dynamic strain can be well reconstructed at the end of a 101.64 km fiber when the signal SNR is down to 3.234 d B.Our proposed method enables the application of the long-distance sensing in quasi-DAS systems. | Guanhua Liang Junfeng Jiang Kun Liu Shuang Wang Tianhua Xu Wenjie Chen Zhe Ma Zhenyang Ding Xuezhi Zhang Yongning Zhang Tiegen Liu | 2020 | Photonics Research2020，8，7： | 6 |
| 12 | Metal-organic frameworks for electrochemical reduction of carbon dioxide: The role of metal centers显示文摘Direct electrochemical reduction of CO2 into valuable chemicals and fuel is one of the most promising approaches to address the current energy crisis and lower CO2 emission.Recently,numerous metal-organic framework(MOF)and their derived materials have extensively been developed as electrocatalysts for CO2 reduction owing to their unique structure including porosity,large specific surface area,and tunable chemical structures.In this review,the recent progress of MOF-based electrocatalysts for CO2 reduction was summarized and discussed.Detailed discussions mainly focus on the synthesis and mechanism of pristine MOFs and MOF-derived materials for electrocatalytic CO2 reduction.These examples are expected to provide clues to rational design and synthesis of stable and high-performance MOFs-based electrocatalysts for CO2 reduction. | Ping Shao Luocai Yi Shumei Chen Tianhua Zhou Jian Zhang | 2020 | Journal of Energy Chemistry2020，29，1： | 6 |
| 13 | NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1显示文摘新兴的数据显示肌动朊动力学与 ciliogenesis 被联系。然而,内在的机制仍然保持不清楚。这里,我们发现那原子分发基因 C (NudC ) , Hsp90 合作女伴,为肌动朊组织和动力学被要求。NudC 的弄空支持睫延伸并且增加 ciliated 房间的百分比。进一步的结果证明那 NudC 绑在并且稳定 cofilin 1,肌动朊动力学的一个关键管理者。cofilin 击倒 1 也便于 ciliogenesis。而且, 1 在 zebrafish,包括的弄弯的身体,心囊的浮肿和有缺陷者引起类似的睫的缺点的 NudC 或 cofilin 的弄空左右的不对称现象。显型在有教养的房间和 zebrafish 由 NudC 弄空导致了的 1 显著地颠倒的 cofilin 的宫外的表示。因此,我们的数据建议 NudC 由稳定 cofilin 调整肌动朊细胞骨架和 ciliogenesis 1。 | Cheng Zhang Wen Zhang Yi Lu Xiaoyi Yan Xiumin Yan Xueliang Zhu Wei Liu YuehongYang Tianhua Zhou | 2016 | Cell Research2016，26，2： | 5 |
| 14 | Emerging roles of NudC family: from molecular regulation to clinical implications显示文摘Nuclear distribution gene C(Nud C) was first found in Aspergillus nidulans as an upstream regulator of Nud F, whose mammalian homolog is Lissencephaly 1(Lis1). Nud C is conserved from fungi to mammals. Vertebrate Nud C has three homologs: Nud C, Nud C-like protein(Nud CL), and Nud C-like protein 2(Nud CL2). All members of the Nud C family share a conserved p23 domain, which possesses chaperone activity both in conjunction with and independently of heat shock protein 90(Hsp90). Our group and the others found that Nud C homologs were involved in cell cycle regulation by stabilizing the components of the LIS1/dynein complex. Additionally, Nud C plays important roles in cell migration, ciliogenesis, thrombopoiesis, and the inflammatory response. It has been reported that Nud CL is essential for the stability of the dynein intermediate chain and ciliogenesis via its interaction with the dynein 2 complex. Our data showed that Nud CL2 regulates the LIS1/dynein pathway by stabilizing LIS1 with Hsp90 chaperone. The fourth distantly related member of the Nud C family, CML66, a tumor-associated antigen in human leukemia, contains a p23 domain and appears to promote oncogenesis by regulating the IGF-1R-MAPK signaling pathway. In this review, we summarize our current knowledge of the Nud C family and highlight its potential clinical relevance. | Qiqin Fu Wei Wang Tianhua Zhou Yuehong Yang | 2016 | Science China(Life Sciences)2016，59，5： | 4 |
| 15 | Monitoring vegetation dynamics in East Rennell Island World Heritage Site using multi-sensor and multi-temporal remote sensing data显示文摘East Rennell of Solomon Island is the first natural site under customary law to be inscribed on UNESCO’s World Heritage List.Potential threats due to logging,mining and agriculture led to the site being declared a World Heritage in Danger in 2013.For East Rennell World Heritage Site(ERWHS)to‘shed’its‘Danger’status the management must monitor forest cover both within and outside of ERWHS.We used satellite data from multiple sources to track forest cover changes for the entire East Rennell island since 1998.95%of the island is still covered by undisturbed forests;annual average normalized difference vegetation index(NDVI)for the whole island was above 0.91 in 2015.However,vegetation cover in the island has been slowly decreasing,at a rate of–0.0011 NDVI per year between 2000 and 2015.This decrease less pronounced inside ERWHS compared to areas outside.While potential threats due to forest clearing outside ERWHS remain the forest cover change from 2000 to 2015 has been below 15%.We suggest ways in which the Government of Solomon Islands could use our data as well as unmanned air vehicles and field surveys to monitor forest cover change and ensure the future conservation of ERWHS. | Mengmeng Wang Guojin He Natarajan Ishwaran Tianhua Hong Andy Bell Zhaoming Zhang Guizhou Wang Meng Wang | 2020 | International Journal of Digital Earth2020，13，3： | 3 |
| 16 | Performance improvement approaches for optical fiber SPR sensors and their sensing applications显示文摘Optical fiber surface plasmon resonance(SPR) sensors point toward promising application potential in the fields of biomarker detection,food allergen screening,and environmental monitoring due to their unique advantages.This review outlines approaches in improving the fiber SPR sensing performance,e.g.,sensitivity,detection accuracy,reliability,cross-sensitivity,selectivity,convenience and efficiency,and corresponding sensing applications.The sensing principles of SPR sensors,especially the performance indicators and their influencing factors,have been introduced.Current technologies for improving the fiber SPR performance and their application scenarios are then reviewed from the aspects of fiber substrate,intrinsic layer(metal layer),and surface nanomaterial modification.Reasonable design of the substrate can strengthen the evanescent electromagnetic field and realize the multi-parameter sensing,and can introduce the in situ sensing self-compensation,which allows corrections for errors induced by temperature fluctuation,non-specific binding,and external disturbances.The change of the intrinsic layer can adjust the column number,the penetration depth,and the propagation distance of surface plasmon polaritons.This can thereby promote the capability of sensors to detect the large-size analytes and can reduce the full width at half-maximum of SPR curves.The modification of various-dimensionality nanomaterials on the sensor surfaces can heighten the overlap integral of the electromagnetic field intensity in the analyte region and can strengthen interactions between plasmons and excitons as well as interactions between analyte molecules and metal surfaces.Moreover,future directions of fiber SPR sensors are prospected based on the important and challenging problems in the development of fiber SPR sensors. | JIANYING JING KUN LIU JUNFENG JIANG TIANHUA XU SHUANG WANG JINYING MA ZHAO ZHANG WENLIN ZHANG TIEGEN LIU | 2022 | Photonics Research2022，10，1： | 3 |
| 17 | Harness the power of genomic selection and the potential of germplasm in crop breeding for global food security in the era with rapid climate change显示文摘Crop genetic improvements catalysed population growth,which in turn has increased the pressure for food security.We need to produce 70%more food to meet the demands of 9.5 billion people by 2050.Climate changes have posed challenges for global food supply,while the narrow genetic base of elite crop cultivars has further limited our capacity to increase genetic gain through conventional breeding.The effective utilization of genetic resources in germplasm collections for crop improvement is crucial to increasing genetic gain to address challenges in the global food supply.Genomic selection(GS)uses genome-wide markers and phenotype information from observed populations to establish associations,followed by genome-wide markers to predict phenotypic values in test populations.Characterizing an extensive germplasm collection can serve a dual purpose in GS,as a reference population for predicting model,and mining desirable genetic variants for incorporation into elite cultivars.New technologies,such as high-throughput genotyping and phenotyping,machine learning,and gene editing,have great potential to contribute to genomeassisted breeding.Breeding programmes integrating germplasm characterization,GS and emerging technologies offer promise for accelerating the development of cultivars with improved yield and enhanced resistance and tolerance to biotic and abiotic stresses.Finally,scientifically informed regulations on new breeding technologies,and increased sharing of genetic resources,genomic data,and bioinformatics expertise between developed and developing economies will be the key to meeting the challenges of the rapidly changing climate and increased demand for food. | Tianhua He Chengdao Li | 2020 | The Crop Journal2020，8，5： | 3 |
| 18 | Reducing nitrogen application with dense planting increases nitrogen use efficiency by maintaining root growth in a double-rice cropping system显示文摘Rational nitrogen(N) application can greatly increase rice(Oryza sativa L.) yield. However, excessive N input can lead not only to low N use efficiency(NUE) but also to severe environmental pollution.Reducing N application rate with a higher planting density(RNHD) is recommended to maintain rice yield and improve NUE. The effects of RNHD on fertilizer N fate and rice root growth traits remain unclear. We accordingly conducted a two-year field experiment to investigate the influence of RNHD on rice yield, fertilizer 15N fate, and root growth in a double-rice cropping system in China. In comparison with the conventional practice of high N application with sparse planting, RNHD resulted in similar yield and biomass production as well as plant N uptake. RNHD increased agronomic NUEs by 23.3%–31.9%(P < 0.05) and N recovery efficiency by 17.4%–24.1%(P < 0.05). RNHD increased fertilizer 15N recovery rate by 14.5%–34.7%(P < 0.05), but reduced15 N retention rate by 9.2%–12.0%(P < 0.05). Although a reduced N rate led to significantly reduced root length, surface area, volume, and biomass, these root traits were significantly increased by higher planting density. RNHD did not affect these root morphological traits and reduced activities of nitrate reductase(NR) and glutamine synthetase(GS) only at tillering stage. Plant N uptake was significantly positively correlated with these root traits, but not correlated with NR and GS activities. Together, these findings show that reducing N application with dense planting can lead to high plant N uptake by maintaining rice root growth and thus increase NUE. | Jin Chen Xiangcheng Zhu Jiang Xie Guoqiang Deng Tianhua Tu Xianjiao Guan Zhen Yang Shan Huang Xianmao Chen Caifei Qiu Yinfei Qian Caihong Shao Minggang Xu Chunrui Peng | 2021 | The Crop Journal2021，9，4： | 3 |
| 19 | TRIM47 is a novel endothelial activation factor that aggravates lipopolysaccharide-induced acute lung injury in mice via K63-linked ubiquitination of TRAF2显示文摘Endothelial activation plays an essential role in the pathogenesis of sepsis-induced acute lung injury,however,the detailed regulatory mechanisms remain largely unknown.Here,we reported that TRIM47,an E3 ubiquitin ligase of the tripartite motifcontaining protein family,was highly expressed in vascular endothelial cells.TRIM47-deficient mice were effectively resistant to lipopolysaccharide(LPS)-induced acute lung injury and death by attenuating pulmonary inflammation.TRIM47 was upregulated during TNFα-induced endothelial activation in vitro.Knockdown of TRIM47 in endothelial cells inhibited the transcription of multiple pro-inflammatory cytokines,reduced monocyte adhesion and the expression of adhesion molecules,and suppressed the secretion of IL-1βand IL-6 in endothelial cells.By contrast,overexpression of TRIM47 promoted inflammatory response and monocyte adhesion upon TNFαstimulation.In addition,TRIM47 was able to activate the NF-κB and MAPK signaling pathways during endothelial activation.Furthermore,our experiments revealed that TRIM47 resulted in endothelial activation by promoting the K63-linked ubiquitination of TRAF2,a key component of the TNFαsignaling pathway.Taken together,our studies demonstrated that TRIM47 as a novel activator of endothelial cells,promoted LPS-induced pulmonary inflammation and acute lung injury through potentiating the K63-linked ubiquitination of TRAF2,which in turn activates NF-κB and MAPK signaling pathways to trigger an inflammatory response in endothelial cells. | Yisong Qian Ziwei Wang Hongru Lin Tianhua Lei Zhou Zhou Weilu Huang Xuehan Wu Li Zuo Jie Wu Yu Liu Ling-Fang Wang Xiao-Hui Guan Ke-Yu Deng Mingui Fu Hong-Bo Xin | 2022 | Signal Transduction and Targeted Therapy2022，7，6： | 2 |
| 20 | Central role of myeloid MCPIP1 in protecting against LPSinduced inflammation and lung injury显示文摘Although systemic inflammatory responses attributable to infection may lead to significant lung injury,the precise molecular mechanisms leading to lung damage are poorly understood and therapeutic options remain limited.Here,we show that myeloid monocyte chemotactic protein-inducible protein 1(MCPIP1)plays a central role in protecting against LPS-induced inflammation and lung injury.Myeloid-specific MCPIP1 knockout mice developed spontaneous inflammatory syndromes,but at a late age compared to global MCPIP1 knockout mice.Moreover,mice with a myeloid-specific deletion of MCPIP1 were extremely sensitive to LPS-induced lung injury due to overproduction of proinflammatory cytokines and chemokines.We identified C/EBPβand C/EBPδ,two critical transcriptional factors that drive cytokine production and lung injury,as targets of MCPIP1 RNase.LPS administration caused MCPIP1 protein degradation in the lungs.Pharmacological inhibition of MALT1,a paracaspase that cleaves MCPIP1,by MI-2 selectively increased the MCPIP1 protein levels in macrophages and in the lungs.Meanwhile,administration of MI-2 protected mice from LPS-induced inflammation,lung injury and death.Collectively,these results indicate that myeloid MCPIP1 is central in controlling LPS-induced inflammation and lung injury.Pharmacological inhibition of MALT1 protease activity may be a good strategy to treat inflammatory diseases by enhancing MCPIP1 expression in myeloid cells. | Yong Li Xuan Huang Shengping Huang Hui He Tianhua Lei Fatma Saaoud Xiao-Qiang Yu Ari Melnick Anil Kumar Christopher J Papasian Daping Fan Mingui Fu | 2017 | Signal Transduction and Targeted Therapy2017，2，1： | 2 |
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