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| 1 | A nuclear import inhibitory peptide ameliorates the severity of cholecystokinin-induced acute pancreatitis显示文摘AIM: To assess the effect of our novel cell-permeable nuclear factor-kappaB (NF-κB) inhibitor peptide PN50 in an experimental model of acute pancreatitis. PN50 was produced by conjugating the cell-penetrating penetratin peptide with the nuclear localization signal of the NF-κB p50 subunit.METHODS: Pancreatitis was induced in male Wistar rats by administering 2×100 μg/kg body weight of cholecystokininoctapeptide (CCK) intraperitoneally (IP) at an interval of 1 h. PN50-treated animals received 1 mg/kg of PN50 IP 30 min before or after the CCK injections. The animals were sacrificed 4 h after the first injection of CCK.RESULTS: All the examined laboratory (the pancreatic weight/body weight ratio, serum amylase activity,pancreatic levels of TNF-α and IL-6, degree of lipid peroxidation, reduced glutathione levels, NF-κB binding activity, pancreatic and lung myeloperoxidase activity) and morphological parameters of the disease were improved before and after treatment with the PN50 peptide.According to the histological findings, PN50 protected the animals against acute pancreatitis by favoring the induction of apoptotic, as opposed to necrotic acinar cell death associated with severe acute pancreatitis.CONCLUSION: Our study implies that reversible inhibitors of stress-responsive transcription factors like NF-κB might be clinically useful for the suppression of the severity of acute pancreatitis. | Tamás Letoha Csaba Somlai Tamáas Takács Annamária Szabolcs Katalin Jármay Zoltán Rakonczay Jr Péter Hegyi Ilona Varga József Kaszaki István Krizbai Imre Boros Ern(?) Duda Erzsébet Kusz Botond Penke | 2005 | World Journal of Gastroenterology2005，11，7： | 15 |
| 2 | CYP24A1 inhibition facilitates the anti-tumor effect of vitamin D3 on colorectal cancer cells显示文摘AIM:The effects of vitamin D3 have been investigated on various tumors, including colorectal cancer (CRC). 25-hydroxyvitamin-D3-24-hydroxylase (CYP24A1), the enzyme that inactivates the active vitamin D3 metabolite 1,25-dihydroxyvitamin D3 (1,25-D3), is considered to be the main enzyme determining the biological halflife of 1,25-D3. During colorectal carcinogenesis, the expression and concentration of CYP24A1 increases significantly, suggesting that this phenomenon could be responsible for the proposed efficacy of 1,25-D3 in the treatment of CRC. The aim of this study was to investigate the anti-tumor effects of vitamin D3 on the human CRC cell line Caco-2 after inhibition of the cytochrome P450 component of CYP24A1 activity. METHODS:We examined the expression of CYP24A1 mRNA and the effects of 1,25-D3 on the cell line Caco-2 after inhibition of CYP24A1. Cell viability and proliferation were determined by means of sulforhodamine-B staining and bromodeoxyuridine incorporation, respectively, while cytotoxicity was estimated via the lactate dehydrogenase content of the cell culture supernatant. CYP24A1 expression was measured by realtime reverse transcription polymerase chain reaction. A number of tetralone compounds were synthesized to investigate their CP24A1 inhibitory activity. RESULTS:In response to 1,25-D3, CYP24A1 mRNA expression was enhanced significantly, in a time- and dose-dependent manner. Caco-2 cell viability and proliferation were not influenced by the administration of 1,25-D3 alone, but were markedly reduced by coadministration of 1,25-D3 and KD-35, a CYP24A1-inhibiting tetralone. Our data suggest that the mechanism of action of co-administered KD-35 and 1,25-D3 does not involve a direct cytotoxic effect, but rather the inhibition of cell proliferation. CONCLUSION:These findings demonstrate that the selective inhibition of CYP24A1 by compounds such as KD-35 may be a new approach for enhancement of the anti-tumor effect of 1,25-D3 on CRC. | János P Kósa Péter Horváth János Wlfling Dóra Kovács Bernadett Balla Péter Mátyus Evelin Horváth Gábor Speer István Takács Zsolt Nagy Henrik Horváth Péter Lakatos | 2013 | World Journal of Gastroenterology2013，19，17： | 5 |
| 3 | Mitochondrial H2O2 generated from electron transport chain complex I stimulates muscle differentiation显示文摘Mitochondrial 反应的氧种类(mROS ) 被认为有害到房间。然而,他们的生理的角色一直不作为表明调停人是彻底地探索了。这里,我们调查了 mROS 是否从 mitochondrial 电子运输链(mETC ) 产生了复杂我刺激了肌肉区别。我们的结果证明 mROS 的数量被增加并且锰 superoxide dismutase (MnSOD ) 在肌肉区别期间经由 NF-B 激活被导致。指向线粒体的抗氧化剂(MitoQ 和 MitoTEMPOL ) 和指向线粒体的过氧化氢酶减少了没有影响 ATP 的数量的 mROS 数量和压制的肌肉区别。Mitochondrial 改变包括在线粒体的数字和尺寸的 mitochondrial 抄写因素 A 和增加的正式就职,并且功能的激活在肌肉区别期间被观察。特别地,增加了 mETC 建筑群的表示层次我子单元和建筑群的一项更高的活动我比另外的建筑群被观察。毒鱼藤素, mETC 建筑群的一个禁止者我,减少 mitochondrial NADH/NAD+ 比率和 mROS 层次在肌肉区别期间。mROS 铺平的我用小介入 RNA 和毒鱼藤素减少了的建筑群的抑制,压制的肌肉区别,和有伴随物的弄空的 ATP 层次在 glycolysis 增加。从这些结果,我们结束那复杂我导出 | Seonmin Lee Eunyoung Tak Jisun Lee MA Rashid Michael P Murphy Joohun Ha Sung Soo Kim | 2011 | Cell Research2011，21，5： | 4 |
| 4 | Novel approaches for the treatment of rheumatoid arthritis:lessons from the evaluation of synovial biomarkers in clinical trials显示文摘 | Gerlag D M Tak P P | 2008 | Best Pract Res Clin Rheumatol2008，22，2： | 1 |
| 5 | NF-KB:a key role in inflammatory diseases 显示文摘 | Tak P P Firestein G S | 2001 | J Clin Invest2001，107，1： | 1 |
| 6 | NF-kappa B:a key role in inflammatory diseases显示文摘 | TAK P P FIRESTEIN G S | 2001 | J Clin Invest2001，107，1： | 1 |
| 7 | The levels of soluble granzyme A and B are elevated in plasma and synovial fluid of patients with rheumatoid arthritis (RA)显示文摘 | Tak P P Spaeny-Dekking L Kraan M C | 1999 | Clin Exp Immunol1999，116，： | 1 |
| 8 | The role of NF -kap- paB activation in the pathogenesis of acute pancreatitis 显示文摘 | Rakonczay Z Jr Hegyi P Tak(zcs T | 2008 | Gut2008，57，2： | 1 |
| 9 | Gustatory perception alterations in obesity: An fMRI study显示文摘 | Csaba Szalay Mihály Aradi Attila Schwarcz Gergely Orsi Gábor Perlaki Lívia Németh Sophia Hanna Gábor Takács István Szabó László Bajnok András Vereczkei Tamás Dóczi József Janszky Sámuel Komoly Péter ?rs Horváth László Lénárd Zoltán Karadi | 2012 | Brain Research2012，，： | 1 |
| 10 | Elevated Levels of Anti- Helicobacter pylori Antibodies in Henoch–Sch?nlein Purpura显示文摘 | János Novák Zoltán Szekanecz Judit Sebesi Alajos Takáts Pál Demeter László Bene Sándor Sipka Zoltán Csiki | 2003 | Autoimmunity2003，，5： | 1 |
| 11 | Fouling control in activated sludge submerged hollow fiber membrane bioreactors显示文摘 | Bae T H Tak T M | 2002 | Desalination2002，143，3： | 1 |
| 12 | NF-~cB: a key role in inflammatory dis eases显示文摘 | Tak P P Firestein G S | 2001 | J Clin Invest2001，107，1： | 1 |
| 13 | Effects of galanin(1-16)on pancreatic secretion in anesthetized and conscious rats显示文摘 | Takács T Hegyi P Czak6 L et al | 2000 | Res Exp Med2000，199，5： | 1 |
| 14 | NF-κB: a key role in inflammatory disease 显示文摘 | TAK P P FIRESTEIN G S | 2001 | J Clin Invest2001，107，1： | 1 |
| 15 | A case of dual infection in a paediatric trauma victim of primary cutaneous aspergillosis caused by Aspergillus flavus and Aspergillus terreus 显示文摘 | Tak V Mathur P Xess I | 2013 | Indian J Med Microhiol2013，31，2： | 1 |
| 16 | NF-kappaB: a key role in inflamma- tory diseases 显示文摘 | Tak P P Firestein G S | 2001 | J Clin Invest2001，107，1： | 1 |
| 17 | Fouling control in activ- ated sludge submerged hollow fiber membrane bioreac- tor显示文摘 | Hong S P Bae T H Tak T M etal | 2002 | Desalination2002，143，21： | 1 |
| 18 | NF-κB:a key role in inflammatory diseases显示文摘 | Tak P P Firestein G S | 2001 | J Clin Investig2001，107，： | 1 |
| 19 | NF-κB:a key role in inflammatory diseases显示文摘 | TAK P P FIRESTEIN G S | 2001 | The Journal of Clinical Investigation2001，107，1： | 1 |
| 20 | Accuracy of multislice computed tomography coronary angiography:a pooled estimate显示文摘 | Gopalakrishnan P Wilson GT Tak T | | 0，，04： | 1 |
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