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| 1 | Bcl-2 degradation is an additional pro-apoptotic effect of polo-like kinase inhibition in cholangiocarcinoma cells显示文摘AIM To examine the influence on apoptotic mechanisms following inhibition of polo-like kinases as therapeuticallyapproach for cholangiocellular cancer treatment. METHODS As most cholangiocarcinomas are chemotherapyresistant due to mechanisms preventing tumor cell death, we investigated the effect of Cisplatin on cholangiocellular carcinoma(CCA) cell lines KMCH-1 and Mz-Ch-1. Polo-like kinases(PLK) are important regulators of the cell cycle and their inhibition is discussed as a potential therapy while PLK inhibition can regulate apoptotic mediators. Here, cells were treated with PLK inhibitor BI6727(Volasertib), Cisplatin, and in combination of both compounds. Cell viability was assessed by MTT; apoptosis was measured by DAPI staining and caspase-3/-7 assay. Western blot and q RT-PCR were used to measure expression levels of apoptosis-related molecules Bax and Bcl-2. RESULTS The cell viability in the CCA cell lines KMCH-1 and Mz-Ch-1 was reduced in all treatment conditions compared to vehicle-treated cells. Co-treatment with BI6727 and cisplatin could even enhance the cytotoxic effect of cisplatin single treatment. Thus, co-treatment of cisplatin with BI6727 could slightly enhance the cytotoxic effect of the cisplatin in both cell lines whereas there was evidence of increased apoptosis induction solely in Mz-Ch-1 as compared to KMCH-1. Moreover, PLK inhibition decreases protein levels of Bcl-2; an effect that can be reversed by the proteasomal degradation inhibitor MG-132. In contrast, protein levels of Bax were not found to be altered by PLK inhibition. These findings indicate that cytotoxic effects of Cisplatin in Mz-Ch-1 cells can be enhanced by co-treatment with BI6727.CONCLUSION In conclusion, BI6727 treatment can sensitize CCA cells to cisplatin-induced apoptosis with proteasomal Bcl-2 degradation as an additional pro-apoptotic effect. | Svenja Sydor Sami Jafoui Lena Wingerter Sandra Swoboda Joachim C Mertens Guido Gerken Ali Canbay Andreas Paul Christian D Fingas | 2017 | World Journal of Gastroenterology2017，23，22： | 5 |
| 2 | Single vs dual(en bloc) kidney transplants from donors ≤ 5 years of age: A single center experience显示文摘AIM: To compare outcomes between single and dual en bloc(EB) kidney transplants(KT) from small pediatric donors. METHODS: Monocentric nonprospective review of KTs from pediatric donors ≤ 5 years of age. Dual EB KT was defined as keeping both donor kidneys attached tothe inferior vena cava and aorta, which were then used as venous and arterial conduits for the subsequent transplant into a single recipient. Donor age was less useful than either donor weight or kidney size in decision-making for kidney utilization as kidneys from donors < 8 kg or kidneys < 6 cm in length were not transplanted. Post-transplant management strategies were standardized in all patients.RESULTS: From 2002-2015, 59 KTs were performed including 34 dual EB and 25 single KTs. Mean age of donors(17 mo vs 38 mo, P < 0.001), mean weight(11.0 kg vs 17.4 kg, P = 0.046) and male donors(50% vs 84%, P = 0.01) were lower in the dual EB compared to the single KT group, respectively. Mean cold ischemia time(21 h), kidney donor profile index(KDPI; 73% vs 62%) and levels of serum creatinine(SCr, 0.37 mg/d L vs 0.49 mg/d L, all P = NS) were comparable in the dual EB and single KT groups, respectively. Actuarial graft and patient survival rates at 5-years follow-up were comparable. There was one case of thrombosis resulting in graft loss in each group. Delayed graft function incidence(12% dual EB vs 20% single KT, P = NS) was slightly lower in dual EB KT recipients. Initial duration of hospital stay(mean 5.4 d vs 5.6 d) and the one-year incidences of acute rejection(6% vs 16%), operative complications(3% vs 4%), and major infection were comparable in the dual EB and single KT groups, respectively(all P = NS). Mean 12 mo SCr and abbreviated MDRD levels were 1.17 mg/d L vs 1.35 mg/d L and 72.5 m L/min per 1.73 m^2 vs 60.5 m L/min per 1.73 m^2(both P = NS) in the dual EB and single KT groups, respectively. CONCLUSION: By transplanting kidneys from young pediatric donors into adult recipients, one can effectively expand the limited donor pool and achieve excellent medium-term outcomes. | Yousef Al-Shraideh Umar Farooq Hany El-Hennawy Alan C Farney Amudha Palanisamy Jeffrey Rogers Giuseppe Orlando Muhammad Khan Amber Reeves-Daniel William Doares Scott Kaczmorski Michael D Gautreaux Samy S Iskandar Gloria Hairston Elizabeth Brim Margaret Mangus Robert J Stratta | 2016 | World Journal of Transplantation2016，6，1： | 3 |
| 3 | Neuropsychological alterations in hepatitis C infection:The role of inflammation显示文摘About 50% of patients with hepatitis C virus(HCV)infection complain of neuropsychiatric symptoms,'brain fog',weakness,fatigue,and exhibit some degree of quality of life impairment,irrespective of the severity of liver disease.Since the first observation of HCV-related cognitive deficits,10 studies have been published that have evaluated neuropsychiatric performance in patients with HCV infection and different degrees of hepatic impairment.Unfortunately,these have often included patients with cirrhosis,patients who had acquired the infection through previous intravenous drug misuse,who had a history of relatively recent treatment with interferon,or were on psychoactive medication.In addition,different neuropsychological batteries and tests that explored different cognitive domains were used,which makes the results of the studies difficult to compare.Finally,limited information is available on the pathogenesis of HCV-related cognitive impairment.Cerebral and/or systemic inflammation may be important players but their potential role has not been substantiated by experimental data.The present review outlines the available evidence of the presence of cognitive impairment in patients with HCV infection,with a focus on the potential relationship with cerebral and/or systemic inflammation. | Marco Senzolo Sami Schiff Cristina Maria D’Aloiso Chiara Crivellin Evangelos Cholongitas Patrizia Burra Sara Montagnese | 2011 | World Journal of Gastroenterology2011，17，29： | 2 |
| 4 | Application of the three-dimensional finite-difference time-domain method to the analysis of planar microstrip circuits 显示文摘 | Sheen D M Sami M A Abouzahra M D | 1990 | IEEE Transactions on MTT1990，38，7： | 1 |
| 5 | Application of the three-dimensional finite-difference time-domain method to the analysis of planar microstrip circuits 显示文摘 | Sheed D M Sami M A | 1990 | IEEE Transactions on Microwave Theory and Techniques1990，38，7： | 1 |
| 6 | Functionalization at C-3a of tryptophan derived hexahydropyrrolo in- doles显示文摘 | BRUNCKO M CRICH D SAMY R | 1993 | Heterocycles1993，36，8： | 1 |
| 7 | Diffuse subcutaneous emphysema after endoscopic retrograde cholangiopancreatography with subsequent pneumothorax and pneumomediastinum显示文摘 | Samies N Reidman D Franga D | 2015 | Am Surg2015，81，2： | 1 |
| 8 | Transparent silica gel-PMMA composites显示文摘 | Pope E J A A Sami M Mackenzie J D | 1989 | J Mater Res1989，，4： | 1 |
| 9 | Repeatability ofpachymetric mapping using fourier domain optica] coherencetomography in corneas with opacities显示文摘 | Samy El Gendy NM Li Y Zhang X Huang D | 2012 | Conwa2012，31，4： | 1 |
| 10 | Study of Viscosity and Thermal Conductivity Effects on Condensation Characteristics of Some New Alternative Refrigerant Mixtures显示文摘 | SAMI S M COMEAU J D | 2003 | Energy Research2003，27，2： | 1 |
| 11 | Characterization of conduction in the ventricles of normal and heterozygous Cx43 knockout mice using optical mapping显示文摘 | Morley GE Vaidya D Samie FH | 1999 | J Cardiovasc Electrophysiol1999，10，10： | 1 |
| 12 | Boiling characteristics of alternatives to R-502 inside air/refrigerant enhanced surface tubing显示文摘 | DESJARDINS D E | 2000 | Int J Ene Res2000，2337，： | 1 |
| 13 | Unusual presentation of eosinophilic fasciitis:two case reports and a review of the literature显示文摘 | Ramazan D Sami A Abdullah A | 2010 | Joumal of Medical Case Reports2010，4，： | 1 |
| 14 | Survey and application of sensor fault detection and isolation schemes显示文摘 | Samy I Postlethwaite I Gu D W | 2011 | Control En- gineering Practice2011，19，7： | 1 |
| 15 | Sensor fault detection and accommodation using neural networks with application to a non-linear unmanned air vehicle model 显示文摘 | Samy I Postlethwaite I Gu D W | 2010 | Proceedings of the Institution of Mechanical Engineers Part G: Journal of Aerospace Engineering2010，224，4： | 1 |
| 16 | Minimum principle and related numerical scheme for simulating initial flow and subsequent propagation of liquefied ground 显示文摘 | M Sami D Buhan Patrick | 2005 | International Journal for Numerical and Analytical Methods in Geomechanics (S0363-9061)2005，11，29： | 1 |
| 17 | Characterization of conduction in the ventricles of normal and geterozygous Cx43 knockout mice using optical mapping显示文摘 | VAIDYA D SAMIE FH | 1999 | J Cardiovasc Electrophysiol1999，10，： | 1 |
| 18 | Low-power data forwarding for VLIW embedded architectures显示文摘 | Sami M Sciuto D Zaccaria V | 2002 | IEEE Transactions on Very Large Scale Integration Systems2002，10，5： | 1 |
| 19 | Tumor Characteristics and Clinical Outcome of Elderly Women With Breast Cancer显示文摘 | SAMI G D RICHARD M E GARY M C | 2000 | Natl Cancer Inst2000，92，： | 1 |
| 20 | Evaluating the performance of a determinisc daily rainfall-runoff model in a low-flow context显示文摘 | Smakhtin V Y Sami K Hughes D A | 1998 | Hydrological Processes1998，12，： | 1 |
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