|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Evaluation of clinical relevance of examining K-ras, p16 and p53 mutations along with allelic losses at 9p and 18q in EUS-guided fine needle aspiration samples of patients with chronic pancreatitis and pancreatic cancer显示文摘AIM: To establish an optimum combination of molecular markers resulting in best overall diagnostic sensitivity and specificity for evaluation of suspicious pancreatic mass. METHODS: Endoscopic ultrasound (EUS)-guided fine needle aspiration cytology (FNA) was performed on 101 consecutive patients (63 males, 38 females, 60 ± 12 years; 81 with subsequently diagnosed pancreatic cancer, 20 with chronic pancreatitis) with focal pancreatic mass. Samples were evaluated on-site by an experienced cytopathologist. DNA was extracted from Giemsa stained cells selected by laser microdissection and the presence of K-ras, p53 and p16 somatic mutations was tested by cycling-gradient capillary electrophoresis (CGCE) and single-strand conformation polymorphism (SSCP) techniques. In addition, allelic losses of tumor suppressor genes p16 (INK4, CDKN2A) and DPC4 (MADH4, SMAD4) were detected by monitoring the loss of heterozygosity (LOH) at 9p and 18q, respectively. RESULTS: Sensitivity and specificity of EUS-guided FNA were 75% and 85%, positive and negative predictivevalue reached 100%. The remaining 26% samples were assigned as inconclusive. Testing of molecular markers revealed sensitivity and specificity of 70% and 100% for K-ras mutations (P < 0.001), 24% and 90% for p53 mutations (NS), 13% and 100% for p16 mutations (NS), 85% and 64% for allelic losses at 9p (P < 0.001) and 78% and 57% for allelic losses at 18q (P < 0.05). When tests for different molecular markers were combined, the best results were obtained with K-ras + LOH at 9p (92% and 64%, P < 0.001), K-ras + LOH at 18q (92% and 57%, P < 0.001), and K-ras + LOH 9q + LOH 18q (96% and 43%, P < 0.001). When the molecular markers were used as complements to FNA cytology to evaluate inconclusive samples only, the overall sensitivity of cancer detection was 100% in all patients enrolled in the study. CONCLUSION: EUS-guided FNA cytology combined with screening of K-ras mutations and allelic losses of tumor suppressors p16 and DPC4 represents a very sensitive approach in screening for pancreatic malignancy. Molecular markers may find its use particularly in cases where FNA cytology has been inconclusive. | C Salek L Benesova M Zavoral V Nosek L Kasperova M Ryska R Strnad E Traboulsi M Minarik | 2007 | World Journal of Gastroenterology2007，13，27： | 18 |
| 2 | Natural orifice transluminal endoscopic surgeryvs laparoscopic ovariectomy:Complications and inflammatory response显示文摘AIM:To compare natural orifice transluminal endoscopic surgery (NOTES) vs standard laparoscopic ovariectomy in mini pigs with respect to technical aspects,complications and parameters of systemic inflammatory response.METHODS:This was a randomized,experimental,survival study.Ten female mini pigs underwent NOTES transgastric ovariectomy (NOTES group) and ten female mini pigs underwent laparoscopic ovariectomy (LAP group).A 'percutaneous endoscopic gastrostomy' approach with guidewire and sphincterotome was used for gastrotomy creation.The ovary was resected using standard biopsy forceps and a snare.The access site was closed using a 'KING' closure with a single endoloop and several clips.In the laparoscopic group,a three-port laparoscopy and an ovariectomy were performed with the use of standard laparoscopic devices.C-reactive protein (CRP),white blood count and interleukin (IL)-6 plasma levels were used as indicators of systemic inflammatory response.All animals were euthanized 28 d after surgery.RESULTS:All animals survived without complications.The mean procedure time was 41.3 min ± 17.6 min (NOTES group) and 25.7 min ± 5.25 min (LAP group,P < 0.02).Postmortem examinations demonstrated that 50% and 70% of animals were free of any complications in the NOTES and LAP groups,respectively.The remaining animals developed minor complications (adhesions) in a comparable frequency between the two groups.In the NOTES group,one animal developed a small intramural gastric abscess close to the gastrotomy site.A minor serous exudate that was present in 50% and 40% of the animals in the NOTES and laparoscopy groups,respectively,was not considered a complication.In both groups CRP levels increased significantly on the 2nd and 7th postoperative days (POD) and returned to normal after 28 d.On POD 2,an increase of CRP level was significantly higher in the NOTES group compared to the LAP group.Values of IL-6 did not differ from baseline values in either of the groups postoperatively.Interestingly,the platelet count decreased significantly on POD 2,but returned close to baseline values on POD 7 and PODs 28-30.CONCLUSION:Both NOTES and laparoscopic ovariectomies had a similar frequency of minor complications.However,the NOTES technique produced an increased systemic inflammatory response on POD 2. | Jan Martínek Ondej Ryska Tereza Filípková Radek Dolezel Stefan Juhas Jan Motlík Monika Holubová Vladimír Nosek Barbora Rotnáglová Miroslav Zavoral Miroslav Ryska | 2012 | World Journal of Gastroenterology2012，18，27： | 4 |
| 3 | Significance of postoperative follow-up of patients with metastatic colorectal cancer using circulating tumor DNA显示文摘BACKGROUND One of the most notable applications for circulating tumor DNA(ctDNA)detection in peripheral blood of patients with metastatic colorectal cancer(mCRC)is a long-term postoperative follow-up.Sometimes referred to as a“liquid(re)biopsy”it is a minimally invasive procedure and can be performed repeatedly at relatively short intervals(months or even weeks).The presence of the disease and the actual extent of the tumor burden(tumor mass)within the patient’s body can be monitored.This is of particular importance,especially when evaluating radicality of surgical treatment as well as for early detection of disease progression or recurrence.AIM To confirm the radicality of surgery using ctDNA and compare available methods for detection of recurrence in metastatic colorectal cancer.METHODSA total of 47 patients with detected ctDNA and indications for resection of mCRC were enrolled in the multicenter study involving three surgical centers.Standard postoperative follow-ups using imaging techniques and the determination of tumor markers were supplemented by ctDNA sampling.In addition to the baseline ctDNA testing prior to surgery,a postoperative observation was conducted by evaluating ctDNA presence up to a week after surgery and subsequently at approximately three-month intervals.The presence of ctDNA was correlated with radicality of surgical treatment and the actual clinical status of the patient.RESULTS Among the monitored patients,the R0(curative)resection correlated with postoperative ctDNA negativity in 26 out of 28 cases of surgical procedures(26/28,93%).In the remaining cases of R0 surgeries that displayed ctDNA,both patients were diagnosed with a recurrence of the disease after 6 months.In 7 patients who underwent an R1 resection,4 ctDNA positivities(4/7,57%)were detected after surgery and associated with the confirmation of early disease recurrence(after 3 to 7 months).All 15 patients(15/15,100%)undergoing R2 resection remained constantly ctDNA positive during the entire follow-up period.In 22 cases of recurrence,ctDNA positivity was detected 22 times(22/22,100%)compared to 16 positives(16/22,73%)by imaging methods and 15 cases(15/22,68%)of elevated tumor markers.CONCLUSION ctDNA detection in patients with mCRC is a viable tool for early detection of disease recurrence as well as for confirmation of the radicality of surgical treatment. | Lucie Benešová Tereza Hálková Renata Ptáčková Anastasiya Semyakina Kateřina Menclová JiříPudil Miroslav Ryska Miroslav Levý JaromírŠimša Filip Pazdírek JiříHoch Milan Blaha Marek Minárik | 2019 | World Journal of Gastroenterology2019，25，48： | 3 |
| 4 | Artificial liver support system reduces intraeranial pressure more effectively than bioartifieial system: an experimental study 显示文摘 | Ryska O Pantoflicek T Laszikova E | 2012 | Int J Artif Organs2012，35，7： | 1 |
| 5 | Expression of galectin-3, cytok- eratin 19, neural cell adhesion molecule and E-cadhedrin in cer- tain variants of papillary thyroid carcinoma 显示文摘 | Laco J Ryska A C6p J | 2008 | Cesk Patol2008，44，4： | 1 |
| 6 | Surgical treatment of pancreatic carcinoma显示文摘 | Záruba P Ryska M | 2012 | Rozhl Chir2012，91，12： | 1 |
| 7 | Expression of galectin-3,cytokeratin19,neural cell adhesion molecule and E-cadhedrin in certain variants of papillary thyroid carcinoma显示文摘 | Laco J Ryska A Cap J | 2008 | Cesk Patol2008，44，4： | 1 |
| 8 | Duodenopancreatectomy versus duo-denum-preserving pancreatic head excision for chronic pancreatitis显示文摘 | Belina F Fronek J Ryska M | 2005 | Pancreatology2005，5，6： | 1 |
| 9 | Chronic sclerosing sialadenitis asone of the immunoglobulin G4-related diseases: a clinicopathologicalstudy of six cases from Central Europe显示文摘 | Laco J Ryska A Celakovsky P | 2011 | Histopathology2011，58，7： | 1 |
| 10 | Expression of galectin-3,cytokeratin 19,neural cell adhesion molecule and E-cadhedrin in certain variants of papillary thyroid carcinoma显示文摘 | Laco J Ryska A Cáp J Celakovsky P | 2008 | Cesk Patol2008，44，4： | 1 |
| 11 | The role of ERCP in management of biliary complications after liver transplantation 显示文摘 | Spicak J Ryska M Trunecka P | 2001 | Ann Transplant2001，6，2： | 1 |
| 12 | Diagnostic role of mark- ers dipeptidyl peptidase IV and thyroid peroxidase in thyroid tumors显示文摘 | Kholov I Ludvikova M Ryska A | 2003 | Anticancer Res2003，23，2： | 1 |
| 13 | Duodenopancreatectomy versus duodenum-preserving pancreatic head excision for chronic pancreatitis 显示文摘 | Belina F Fronek J Ryska M | 2005 | Pancreatology2005，5，6： | 1 |
| 14 | Matrix-producing breast carcinoma with myoepithelial differentiation-description of 11 cases and review of literature aimed at histogenesis and differential diagnosis 显示文摘 | Kinkor Z Baudova L Ryska A | 2004 | Ceska Gyneko12004，69，3： | 1 |
| 15 | Compliance with theprocedures ofmodern perioperative care(Enhanced Recovery After Surgery) atsurgery departments in the Czech Republic - results of a nationalsurvey显示文摘 | Ryska O Serclova Z Antoa F | 2013 | RozhlChir2013，92，8： | 1 |
| 16 | The role of dispositional goal orientation and team climate on situational self-handicapping among young athletes显示文摘 | Ryska T A Yin Z & Boyd M | 1999 | Journal of Sport Behaviour1999，22，： | 1 |
| 17 | The role ofdispositional goal orientation and team on situational self-handicapping among young athletes显示文摘 | Ryska T A YinZ BoydM | 1999 | Journal of Sport Behavior1999，22，： | 1 |
| 18 | Sensitivity and specificity of the fine needle aspiration biopsy of the thyroid显示文摘 | Cap J Ryska A Rehorkova P | 1999 | Clin Endocrinol (Oxf)1999，51，： | 1 |
| 19 | Oncocytic papillary carcinoma with lymphoid stroma ( Warthin-like turnout) of the thyroid: a distinct entity with favourable prognosis 显示文摘 | Ludvlkov~t M Ryska A Korabecn6 M et 02 | 2001 | Histopathology2001，39，1： | 1 |
| 20 | Sensitivity and specificity of the fine needle aspiration biopsy of the thyroid:clinical point of view显示文摘 | Cap J Ryska A Rehorkova P | 1999 | Clin Endocrinol1999，51，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员