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| 1 | 利用RNA干扰技术抑制水稻淀粉极限糊精酶基因表达显示文摘构建了含极限糊精酶基因片段反向重复结构的RNAi载体,抑制水稻籽粒中极限糊精酶基因表达获得了极限糊精酶基因功能缺失突变体。经酶切及序列测定证实,成功地构建了胚乳特异表达启动子启动的水稻极限糊精酶基因反向重复结构RNAi载体;通过根癌农杆菌(Agrobacterium tumefaciens)介导水稻(Oryza sativa sp.japonica)转化,经PCR及Southern杂交检测,获得28个水稻转基因再生株系;以极限糊精酶抗体对转基因株系胚乳发育期籽粒蛋白质进行Western杂交,并经SDS-PAGE极限糊精酶活性检测,结果显示,该反向重复结构在胚乳发育期表达,减少了极限糊精酶的积累,获得了不同沉默效果的转基因株系。转化株系012的极限糊精酶活性只有野生型的10%,表明该载体可有效地抑制水稻胚乳中极限糊精酶的活性。 | 黄冰艳 李忠谊 吉万全 海燕 Fleming J Morell M Rahman S | 2007 | 农业生物技术学报2007，15，1： | 13 |
| 2 | Prospective randomized controlled trial evaluating cap-assisted colonoscopy vs standard colonoscopy显示文摘AIM: To study the significance of cap-fitted colonoscopy in improving cecal intubation time and polyp detection rate. METHODS: This study was a prospective randomized controlled trial conducted from March 2008 to February 2009 in a tertiary referral hospital at Sydney. The primary end point was cecal intubation time and the secondary endpoint was polyp detection rate. Consecutive cases of total colonoscopy over a 1-year period were recruited. Randomization into either standard colonoscopy (SC) or cap-assisted colonoscopy (CAC) was performed after consent was obtained. For cases randomized to CAC, one of the three sizes of cap was used: D-201-15004 (with a diameter of 15.3 mm), D-201-14304 (14.6 mm) and D-201-12704 (13.0 mm). All of these caps were produced by Olympus Medical Systems, Japan. Independent predictors for faster cecal time and better polyp detection rate were also determined from this study. RESULTS: There were 200 cases in each group. There was no signif icant difference in terms of demographic characteristics between the two groups. CAC, when compared to the SC group, had no signif icant difference in terms of cecal intubation rate (96.0% vs 97.0%, P = 0.40) and time (9.94 ± 7.05 min vs 10.34 ± 6.82 min, P = 0.21), or polyp detection rate (32.8% vs 31.3%, P = 0.75). On the subgroup analysis, there was no significant difference in terms of cecal intubation time by trainees (88.1% vs 84.8%, P = 0.40), ileal intubation rate (82.5% vs 79.0%, P = 0.38) or total colonoscopy time (23.24 ± 13.95 min vs 22.56 ± 9.94 min, P = 0.88). On multivariate analysis, the independent determinants of faster cecal time were consultant-performed procedures (P < 0.001), male patients (P < 0.001), non-usage of hyoscine (P < 0.001) and better bowel preparation (P = 0.01). The determinants of better polyp detection rate were older age (P < 0.001), no history of previous abdominal surgery (P = 0.04), patients not having esophagogastroduodenoscopy in the same setting (P = 0.003), trainee-performed procedures (P = 0.01), usage of hyoscine (P = 0.01) and procedures performed for polyp follow-up (P = 0.01). The limitations of the study were that it was a single-center experience, no blinding was possible, and there were a large number of endoscopists. CONCLUSION: CAC did not signif icantly different from SC in term of cecal intubation time and polyp detection rate. | Hoi-Poh Tee Crispin Corte Hamdan Al-Ghamdi Emilia Prakoso John Darke Raman Chettiar Wassim Rahman Scott Davison Sean P Griffin Warwick S Selby Arthur J Kaffes | 2010 | World Journal of Gastroenterology2010，16，31： | 10 |
| 3 | Drainage vs. non-drainage after cholecystectomy for acute cholecystitis:a retrospective study显示文摘Many surgeons practice prophylactic drainage after cholecystectomy without reliable evidence,this study was conducted to answer the question whether to drain or not to drain after cholecystectomy for acute calculous cholecystitis.A retrospective review of all patients who had cholecystectomy for acute cholecystitis in Aseer Central Hospital,Abha,Saudi Arabia,was conducted from April 2010 to April 2012.Data were extracted from hospital case files.Preoperative data included clinical presentation,routine investigations and liver function tests.Operative data included excessive adhesions,bleeding,bile leak,and drain insertion.Complicated cases such as pericholecystic collections,mucocele and empyema were also reported.Patients who needed therapeutic drainage were excluded.Postoperative data included hospital stay,volume of drained fluid,time of drain removal,and drain site problems.The study included 103 patients allocated into two groups;group A(n = 38) for patients with operative drain insertion and group B(n = 65) for patients without drain insertion.The number of patients with preoperative diagnosis of acute non-complicated cholecystitis was significantly greater in group B(80%) than group A(36.8%)(P < 0.001).Operative time was significantly longer in group A.All patients who were converted from laparoscopic to open cholecystectomy were in group A.Multivariate analysis revealed that hospital stay was significantly(P < 0.001) longer in patients with preoperative complications.There was no added benefit for prophylactic drain insertion after cholecystectomy for acute calculous cholecystitis in non-complicated or in complicated cases. | Mohammed A Bawahab Walid M Abd El Maksoud Saeed A Alsareii Fahad S Al Amri Hala F Ali Abdul Rahman Nimeri Abdul Rahman M Al Amri Adel A Assiri Mohammed I Abdul Aziz | 2014 | The Journal of Biomedical Research2014，28，3： | 3 |
| 4 | Challenges in laser-assisted milling of titanium alloys显示文摘Several detailed studies have comprehensively investigated the benefits and limitations of laser-assisted machining(LAM)of titanium alloys.These studies have highlighted the positive impact of the application of laser preheating on reducing cutting forces and improving productivity but have also identified the detrimental effect of LAM on tool life.This paper seeks to evaluate a series of the most common cutting tools with different coating types used in the machining of titanium alloys to identify whether coating type has a dramatic effect on the dominant tool wear mechanisms active during the process.The findings provide a clear illustration that the challenges facing the application of LAM are associated with the development of new types of cutting tools which are not subjected to the diffusion-controlled wear processes that dominate the performance of current cutting tools. | Matthew S Dargusch Tharmalingam Sivarupan Michael Bermingham Rizwan Abdul Rahman Rashid Suresh Palanisamy Shoujin Sun | 2021 | International Journal of Extreme Manufacturing2021，3，1： | 3 |
| 5 | Environmental impact assessment on water quality deterioration caused by the decreased Ganges outflow and saline water intrusion in south -western Bangladesh 显示文摘 | Rahman M M Hassan M Q Islam M S | 2000 | Environ Geol2000，40，12： | 2 |
| 6 | The major proteins of wheat endosperm starch granules显示文摘 | Rahman S Kosar-Hashemi B Sameul M S | 1995 | Austr J Plant ysiol1995，22，： | 2 |
| 7 | Analysis and evaluation of five short-term load forecastingtechniques显示文摘 | Moghram I Rahman S | 1989 | IEEE Trans on Power Systems1989，4，4： | 2 |
| 8 | Review of usage- based transmission cost allocation methods under open access 显示文摘 | PAN Jiuping TKELU Y RAHMAN S | 2000 | IEEE Trans on Power Systems2000，15，4： | 2 |
| 9 | An expert system based algorithm for short term load forecast显示文摘 | Rahman S Bhatnagar R | 1988 | IEEE Trans on Power Systems1988，3，2： | 2 |
| 10 | Lunar cycles and reproducetive activity in reef fishes with particular attention to rabbitfishes 显示文摘 | TAKEMURA A RAHMAN M S NAKAMURA S | 2004 | Fish and fisheries2004，，5： | 1 |
| 11 | Nested PCR for the rapid detection of TB from pleural fluid at HUKM Malaysia 显示文摘 | Salleh SA Hussin S Rahman MM | 2008 | Pak J Biol Sci2008，11，13： | 1 |
| 12 | Review of usage-based transmission cost allocation methods under open access显示文摘 | Teklu Y Rahman S | 2000 | IEEE Trans On Power Systems2000，15，4： | 1 |
| 13 | Anti-tuberculosis activity of quassinoids显示文摘 | Rahman S Fukamiya N Okano M | 1997 | Chem Pharm Bull1997，45，9： | 1 |
| 14 | Optimized torque control of switched reluctance motor at all operational regimes using neural network 显示文摘 | RAHMAN K M GOPALAKRISHNAN S FAHIMI B | 2001 | IEEE Transactions on Industrial Applications2001，37，3： | 1 |
| 15 | Wernicke encephalopathy in nonalcoholic patients显示文摘 | Ogershok PR Rahman A Nestor S | 2002 | Am J Med Sci2002，323，2： | 1 |
| 16 | ' Abasin -95' , a new oilseed rape cultivar developed through induced mutations 显示文摘 | Shah S A Ali I Rahman K | 2001 | Mut Breed Newsletter2001，45，： | 1 |
| 17 | Experimental study of micro and nano-scale cutting of aluminum 7075-T6 显示文摘 | Ng C K Melkote S N Rahman M | 2006 | Int J Mach Tools Manuf (S0890-6955)2006，46，9： | 1 |
| 18 | DynamicYoung's modulus and dimensional stability of Batai tropical wood impregnated with polyvinyl alcohol显示文摘 | ISLAM M S HAMDAN S RAHMAN M R | 2010 | Journal of Scientific Research2010，2，2： | 1 |
| 19 | Desorption isotherm and heat pump drying kinetics of peas显示文摘 | Rahman M S Perera C O Theband C | 1998 | Food Research International1998，30，： | 1 |
| 20 | An element-free Galerkin method for probabilistic mechanics and reliability 显示文摘 | Rahman S Rao B N | 2001 | International Joumal of Solids and Structures2001，38，： | 1 |
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