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| 1 | Tumor Antigen Specific Activation of Primary Human T-Cells Expressing a Virally Encoded Chimeric T-Cell Receptor Specific for p185HER2显示文摘We have developed and tested chimeric T-cell receptors (TCR) specific for p185HER2. In these experiments, retroviral vectors expressing the N29γ or N29ζ receptors were constructed in pRET6. Amphotropic viral producer cells were established in the GALV-based PG13 packaging cell line. Ficoll purified human peripheral blood lymphocytes (PBL) were virally transduced using an optimized protocol incorporating activation with immobilized anti-CD3/anti-CD28 monoclonal anti- bodies, followed by viral infection in the presence of fibronectin fragment CH296. Transduced cells were co-cultured with human tumor cell lines that overexpress (SK-OV-3) or underexpress (MCF7) p185HER2 to assay for antigen specific im- mune responses. Both CM+ and CD8+ T-cells transduced with the N29γ or N29ζ chTCR demonstrated HER2-specific anti- gen responses, as determined by release of Th1 like cytokines, and cellular cytotoxicity assays. Our results support the fea- sibility of adoptive immunotherapy with genetically modified T-cells expressing a chTCR specific for p185HER2. | 杨建民 Michael S FRIEDMAN Christopher M REYNOLDS Marianne T HUBEN Lee WILKE Jennifer FULLER 李桥 Zelig ESHHAR James J MULE Kevin T MCDONAGH | 2004 | Journal of Microbiology and Immunology2004，2，4： | 5 |
| 2 | In vivo anti-tumor activity of murine hematopoietic stem cells expressing a p185HER2-specific chimeric T-cell receptor gene显示文摘We have confirmed efficient anti-tumor activities of the peripheral lymphocytes transduced with a p185HER2-specific chimeric T-cell receptor gene both in murine and in human in our previous studies. To further test the feasibility of chimeric T-cell receptor in a bone marrow transplantation model, we first, made two murine tumor cell lines: MT901 and MCA-205, to express human p185HER2 by retroviral gene transduction. Murine bone marrow cells were retrovirally transduced to express the chimeric T-cell receptor and gene-modified bone marrow cells were transplanted into lethally irradiated mouse. Six months post transplantation, p185HER2-positive tumor cells:MT-901/HER2 or MCA-205/ HER2 was subcutaneously or intravenously injected to make mouse models simulating primary breast cancer or pulmonary metastasis. The in vivo anti-tumor effects were monitored by the size of the subcutaneous tumor or counting the tumor nodules in the lungs after India ink staining. The size of the subcutaneous tumor was significantly inhibited and the number of pulmonary nodules were significantly decreased in mouse recipients transplanted with chimeric T-cell receptor modified bone marrow cells compared with the control group. Our results suggest the efficient in vivo anti-tumor activities of chimeric T-cell receptor gene modified bone marrow cells. | JIAN MIN YANG MICHAEL S FRIEDMAN MARIANNE T HUBEN JENNIFER FULLER QIAO LI ALFRED E CHANG JAMES J MULE KEVIN T MCDONAGH | 2006 | Journal of Microbiology and Immunology2006，4，2： | 3 |
| 3 | 转染嵌合性T细胞受体基因小鼠T淋巴细胞的体外抗肿瘤作用显示文摘目的:研究小鼠T淋巴细胞在转染嵌合性T细胞受体基因后的体外抗肿瘤作用。方法:应用重组DNA技术,将HER2特异性嵌合性T-细胞受体构建入逆转录病毒载体;转入包装细胞后,收集病毒上清,转染小鼠T淋巴细胞,转基因后的小鼠T淋巴细胞分别与HER2阳性(SK-OV-3)或阴性(MCF-7)的肿瘤细胞系共培养,检测其细胞因子γ干扰素释放,51Cr释放法检测CTL评价其抗肿瘤效应。结果:所构建载体经酶切鉴定符合要求,乒乓法转染包装细胞系GP+E86,检测病毒滴度为1.2×106,Retronectin结合离心法转染经抗CD3/CD28单抗活化的小鼠T淋巴细胞,转染效率可达50%以上;转染嵌合性T细胞受体基因的T淋巴细胞与HER2阳性或阴性的肿瘤细胞系共培养后可检测到HER2特异性的细胞因子γ干扰素释放,51Cr释放法测CTL可见转染嵌合性T细胞受体基因T淋巴细胞对HER2阳性的肿瘤细胞具显著杀伤效应。结论:转染嵌合性T细胞受体基因的小鼠T淋巴细胞在体外可通过细胞因子释放和CTL效应发挥显著的抗肿瘤作用。 | 杨建民 Michael S Friedman 李峤 James J Mule Alfred E Chang Kevin T McDonagh | 2006 | 中国肿瘤生物治疗杂志2006，13，4： | 2 |
| 4 | Microalbuminuria and early endothelial activation in essential hypertension显示文摘 | Mule G Nardi E | 2007 | J Hum Hypertens2007，21，： | 1 |
| 5 | The economic impact of special events : the use of forecasts 显示文摘 | Mules T McDonald S | 1994 | Festival Management and Event Tourism: An International Journal1994，，1： | 1 |
| 6 | Relation of C-reactive protein to oxidative stress and to endothelial activation in essential hypertension显示文摘 | Cottone S Mule G Nardi E | 2006 | Am J Hypertens2006，19，3： | 1 |
| 7 | Relation of C - reative protein to oxidative stress and to endothelial activation in essential hypertension 显示文摘 | Cottone S Mule G Nardi E | 2006 | Am J Hypertens2006，19，3： | 1 |
| 8 | Microalbuminuria, renal dysfunction and cardiovascular complication in essential hypertension显示文摘 | CERASOLA G COTTONE S MULE G | 1996 | J Hypertens1996，14，7： | 1 |
| 9 | The economic impact of special events: the use of forecasts 显示文摘 | Mules T & McDonald S | 1994 | Festival Management and Event Tourism : An International Journal1994，2，1： | 1 |
| 10 | Relation of C-reactive protein to exidative stress and to endothelial activation in essential hypertension显示文摘 | Cottone S Mule G Nardi E | 2006 | Am J Hypertens2006，19，3： | 1 |
| 11 | Involvement of tachykinin NK2 receptors in the modulation of spontaneous motility in rat proximal colon显示文摘 | MULE F D' ANGELO S TABACCHI G | 2000 | Neurogastroenterol Motil2000，12，5： | 1 |
| 12 | Microalbu- minuria and early endothelial activation in essential hypertension显示文摘 | COTTONE S MULE G NARDI E | 2007 | J Hum Hypertens2007，21，： | 1 |
| 13 | Interleukin 7 generates antitumor cytotoxic T lymphocytes against murine sarcomas with efficacy in cellular adoptive immunotherapy 显示文摘 | Rosenberg S A Jicha D L Mule J J | 1991 | J EXP Med1991，174，12： | 1 |
| 14 | Current developments of immunotherapy in the clinic 显示文摘 | ANTONIA S MULE JJ WEBER JS | 2004 | Curt Opin Immunol2004，16，2： | 1 |
| 15 | epigallocatechin-3-gaUate inhibits gelatinase activity of some bacterial isolates fromocular infection, and limits their invasion through gelatin显示文摘 | BLANCO A R LA TERRA MULE S BABINI G | 2003 | Biophys Acta2003，，1620： | 1 |
| 16 | Microalbuminufia and early endothelial activation in essential hypertension 显示文摘 | Cottone S Mule G Nardi E | 2007 | J Hum Hypertens2007，21，2： | 1 |
| 17 | Relation of C-reactive protein to oxidative stress and to endothelial activation in essential hyper-tension显示文摘 | Cottone S Mule G Nardi E | 2006 | Am J Hypertens2006，19，3： | 1 |
| 18 | Relation of C- reactive protein to oxidative stress and to endothelial activation in essential hypertension 显示文摘 | Cottone S Mule G Nardi E | 2006 | Am J Hyper- tense2006，19，3： | 1 |
| 19 | Metabolic syndrome in subjects with essential hypertension: relationships with subclinical eardiovaseular and renal damage 显示文摘 | Mule G Cottone S Nardi E | 2006 | Minewa Cardioangiologica2006，54，2： | 1 |
| 20 | Inverse relationship between ambulatory arterial stiffness index and glomerular filtration rate in arterial hypertension显示文摘 | Mule G Cottone S Cusimano P | 2008 | Hypertension2008，21，1： | 1 |
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