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| 1 | 临床致病性不动杆菌的分子生物学鉴定显示文摘目的对临床致病性不动杆菌进行种属鉴定,并比较3种方法(44℃生长试验、特异性引物PCR扩增和16SrRNA测序)对鲍曼不动杆菌鉴定的特异性、准确性及应用价值。方法收集临床致病不动杆菌56株,首先接种在胰蛋白胨大豆肉汤中44℃进行培养,观察是否可以生长;其次,提取56株不动杆菌基因组DNA,用两组不同的特异性引物进行PCR扩增鉴定;最后,用通用引物对56株不动杆菌的16SrRNA进行测序鉴定。结果 56株不动杆菌中有17株可在44℃生长。经特异性引物PCR扩增鉴定,发现8株鲍曼不动杆菌和3株13TU型不动杆菌均可在44℃中较早较快生长。金标准16SrRNA测序确定56株不动杆菌中含有9个种,其中鲍曼不动杆菌和13TU型不动杆菌的鉴定结果与特异性引物PCR扩增法相同。结论应用分子生物学方法鉴定鲍曼不动杆菌具有准确、高效且可重复性高的优点,尤其是特异性PCR扩增法,可作为较难诊断的鲍曼不动杆菌的首选鉴定方法。 | 韩蕾 张旭燕 韩少山 MCCARRON Andrew MOORE John E MILLAR Cherie B 徐纪茹 | 2012 | 西安交通大学学报(医学版)2012，33，5： | 4 |
| 2 | Meeting report:a hard look at the state of enamel research显示文摘The Encouraging Novel Amelogenesis Models and Ex vivo cell Lines(ENAMEL) Development workshop was held on 23 June 2017 at the Bethesda headquarters of the National Institute of Dental and Craniofacial Research(NIDCR). Discussion topics included model organisms, stem cells/cell lines, and tissues/3 D cell culture/organoids. Scientists from a number of disciplines,representing institutions from across the United States, gathered to discuss advances in our understanding of enamel, as well as future directions for the field. | ophir d klein olivier duverger wendy shaw rodrigo s lacruz derk joester janet moradian-oldak megan k pugach j timothy wright sarah e millar ashok b kulkarni john d bartlett thomas gh diekwisch pamela den besten james p simmer | 2017 | International Journal of Oral Science2017，9，4： | 3 |
| 3 | Comparison of API 20 C with molecular identification of Candida spp isolated from bloodstream infections显示文摘 | J Xu B C Millar J E Moore | | J Clin Pathol0，55，： | 1 |
| 4 | A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands 显示文摘 | Frommer M McDonald L E Millar D S | 1992 | Proc Natl Acad Sci USA1992，89，： | 1 |
| 5 | A genomie sequen- cing protocol that yields a positive display of 5-methylcytosine resi- dues in individual DNA strands显示文摘 | Frommer M McDonald L E Millar D S | 1992 | Proe Natl Aead Sci U S A1992，89，5： | 1 |
| 6 | Methylation of a CpG island within the promoter region of the KAI1/CD82 metastasis suppressor gene is not responsible for down-regulation of KAI1/CD82 expression in invasive cancers or cancer cell lines显示文摘 | JACKSON P MILLAR D KINGSLEY E | 2000 | Cancer Lett2000，157，2： | 1 |
| 7 | Comparison of API20C with molecular identification of Candida spp isolated from bloodstream infections显示文摘 | J Xu B C Millar J E Moore | 2002 | J Clin Pathol2002，55，8： | 1 |
| 8 | Crystal engineering of energetic materials:cocrystals of CL-20显示文摘 | Millar D I A Maynard-Caely H E Allam D R | 2012 | Cryst Eng Comm2012，14，10： | 1 |
| 9 | How information gives you competitve advantage显示文摘 | PORTER M E MILLAR V E | 1985 | Havard Business Review1985，，： | 1 |
| 10 | Energetic polymers as binders in composite propellants and explosives 显示文摘 | Colclough M E Desai H Millar R W | 1994 | Polymer for Advanced Technologies1994，5，： | 1 |
| 11 | Energetic polymers as binders in composite propellants and explosives 显示文摘 | Colclough M E Desai H Millar R W | 1994 | Polymer for Advanced Technologies1994，5，9： | 1 |
| 12 | The international workshop on meibomian gland dysfunction: report of the subcommittee on anatomy, physiology, and pathophysiology of the meibomian gland 显示文摘 | Knop E Knop N Millar T | 2011 | Invest Ophthalmol Vis Sci2011，52，4： | 1 |
| 13 | Increased atherosclerosis in mice lacking apolipoprotein A-I attributable to both impaired reverse cholesterol transport and increased inflammation显示文摘 | Moore R E Navab M Millar J S | 2005 | Circ Res2005，97，8： | 1 |
| 14 | How information gives you competitive advantage 显示文摘 | PORTER M E MILLAR V E | 1985 | Harvard Business Review1985，，78： | 1 |
| 15 | A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands显示文摘 | Frommer M McDonald L E Millar D S | 1992 | Proc Natl Acad Sci U S A1992，89，5： | 1 |
| 16 | A 1 -year variation in distress, and illness perceptions, after treatment for breast cancer 显示文摘 | Millar K Purushotham AD prospective study of individual McLatchie E | 2005 | J Psychosom Res2005，58，4： | 1 |
| 17 | The Determination of wheat bread-making performance and bread dough mixing time by NIR Spectroscopy for high speed mixers显示文摘 | Alava J M Millar S J Salmon S E | 2001 | Journal of Cereal Science2001，33，： | 1 |
| 18 | Molecular mechanisms regulating hair follicle devel- opment显示文摘 | MILLAR S E | 2002 | J Invest Dermatol2002，118，2： | 1 |
| 19 | New synthesis routes for energetic materials using dinitrogen pentoxide 显示文摘 | Millar R W Colclough M E Golding P | 1992 | Philosophical Transactions of the Royal Society of London1992，339，： | 1 |
| 20 | Male-produced aggre- gation pheromone of the Cerambycid beetle Neoclytus acumina- ms acuminatus 显示文摘 | Lacey E S Ginzel M D Millar J G | 2004 | Journal of Chemical Ecology2004，30，： | 1 |
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