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| 1 | Microbiota modification by probiotic supplementation reduces colitis associated colon cancer in mice显示文摘AIM To investigate the effect of probiotic supplementation during the development of an experimental model of colitis associated colon cancer(CAC). METHODS C57 BL/6 mice received an intraperitoneal injection of azoxymethane(10 mg/kg), followed by three cycles of sodium dextran sulphate diluted in water(5% w/v). Probiotic group received daily a mixture of Lactobacillus acidophilus, Lactobacil us rhamnosus and Bifidobacterium bifidum. Microbiota composition was assessed by 16 Sr RNA Illumina Hi Seq sequencing. Colon samples were collected for histological analysis. Tumor cytokines was assessed by Real Time-PCR(Polymerase Chain Reaction); and serum cytokines by Multiplex assay. All tests were two-sided. The level of significance was set at P < 0.05. Graphs were generated and statistical analysis performed using the software Graph Pad Prism 5.0. The project was approved by the institutional review board committee. RESULTS At day 60 after azoxymethane injection, the mean number of tumours in the probiotic group was 40% lower than that in the control group, and the probiotic group exhibited tumours of smaller size(< 2 mm)(P < 0.05). There was no difference in richness and diversity between groups. However, there was a significant difference in beta diversity in the multidimensional scaling analysis. The abundance of the genera Lactobacillus, Bifidobacterium, Allobaculum, Clostridium XI and Clostridium XVⅢ increased in the probiotic group(P < 0.05). The microbial change was accompanied by reduced colitis, demonstrated by a 46% reduction in the colon inflammatory index; reduced expression of the serum chemokines RANTES and Eotaxin; decreased p-IKK and TNF-α and increased IL-10 expression in the colon. CONCLUSION Our results suggest a potential chemopreventive effect of probiotic on CAC. Probiotic supplementation changes microbiota structure and regulates the inflammatory response, reducing colitis and preventing CAC. | Maria Carolina S Mendes Daiane SM Paulino Sandra R Brambilla Juliana A Camargo Gabriela F Persinoti José Barreto C Carvalheira | 2018 | World Journal of Gastroenterology2018，24，18： | 17 |
| 2 | Titanium dioxide induced inflammation in the small intestine显示文摘AIM:To investigate the effects of titanium dioxide (TiO2) nanoparticles (NPTiO 2 ) and microparticles (MPTiO 2 ) on the inflammatory response in the small intestine of mice. METHODS: Bl 57/6 male mice received distilled water suspensions containing TiO 2 (100 mg/kg body weight) as NPTiO 2 (66 nm), or MPTiO 2 (260 nm) by gavage for 10 d, once a day; the control group received only distilled water. At the end of the treatment the duodenum, jejunum and ileum were extracted for assessment of cytokines, inflammatory cells and titanium content. The cytokines interleukin (IL)-1b, IL-4, IL-6, IL-8, IL-10, IL-12, IL-13, IL-17, IL-23, tumor necrosis factor-α (TNF-α), intracellular interferon-γ (IFN-γ) and transforming growth factor-β (TGF-β) were evaluated by enzyme-linked immunosorbent assay in segments of jejunum and ileum (mucosa and underlying muscular tissue). CD4 + and CD8 + T cells, natural killer cells, and dendritic cells were evaluated in duodenum, jejunum and ileum samples fixed in 10% formalin by immuno-histochemistry. The titanium content was determined by inductively coupled plasma atomic emission spectrometry. RESULTS: We found increased levels of T CD4 + cells (cells/mm 2 ) in duodenum:NP 1240 ± 139.4, MP 1070 ± 154.7 vs 458 ± 50.39 (P < 0.01); jejunum:NP 908.4 ± 130.3, MP 813.8 ± 103.8 vs 526.6 ± 61.43 (P < 0.05); and ileum:NP 818.60 ± 123.0, MP 640.1 ± 32.75 vs 466.9 ± 22.4 (P < 0.05). In comparison to the control group, the groups receiving TiO 2 showed a statistically significant increase in the levels of the inflammatory cytokines IL-12, IL-4, IL-23, TNF-α, IFN-γ and TGF-β. The cytokine production was more pronounced in the ileum (mean ± SE):IL-12: NP 33.98 ± 11.76, MP 74.11 ± 25.65 vs 19.06 ± 3.92 (P < 0.05); IL-4: NP 17.36 ± 9.96, MP 22.94 ± 7.47 vs 2.19 ± 0.65 (P < 0.05); IL-23: NP 157.20 ± 75.80, MP 134.50 ± 38.31 vs 22.34 ± 5.81 (P < 0.05); TNFα: NP 3.71 ± 1.33, MP 5.44 ± 1.67 vs 0.99± 019 (P < 0.05); IFNγ: NP 15.85 ± 9.99, MP 34.08 ± 11.44 vs 2.81 ± 0.69 (P < 0.05); and TGF-β: NP 780.70 ± 318.50, MP 1409.00 ± 502.20 vs 205.50 ± 63.93 (P < 0.05). CONCLUSION:Our findings indicate that TiO2 particles induce a Th1-mediated inflammatory response in the small bowel in mice. | Carolina Maciel Nogueira Walter Mendes de Azevedo Maria Lucia Zaidan Dagli Sérgio Hiroshi Toma AndréZonetti de Arruda Leite Maria Laura Lordello Iêda Nishitokukado Carmen Lúcia Ortiz-Agostinho Maria IrmaSeixas Duarte Marcelo Alves Ferreira Aytan Miranda Sipahi | 2012 | World Journal of Gastroenterology2012，18，34： | 8 |
| 3 | Evo-lution of Networks 显示文摘 | S N DOROGOVTSEV J F F MENDES | 2002 | Adv Phys2002，51，： | 1 |
| 4 | Short- term electricity prices forecasting in a competitive market by a hybrid intelligent approach显示文摘 | Catal:o J P S Pousinho H M I Mendes V M F | 2011 | Energy Conversion and Management2011，52，： | 1 |
| 5 | The role of constraints within generalized nonextensive statistics显示文摘 | Tsallis C Mendes R S Plastino A R | 1998 | Physica A:Statistical Mechanics and its Applications1998，261，3： | 1 |
| 6 | One dimensional model of phase holdups in three phase stratified flow显示文摘 | Khor S H Mendes Tatsis M A Hewitt G F | 1997 | Int J Muhiphase Flow1997，23，5： | 1 |
| 7 | Evolving of Networks显示文摘 | S N Dorogovtsev J F F Mendes | 2002 | Adv Physics2002，51，： | 1 |
| 8 | The Effect of a Psychosocial Intervention and Quality of Life after Acute Myocardial Infarction: The Enhancing Recovery in Coronary Heart Disease (ENRICHD) Clinical Trial显示文摘 | Mendes de Leon C F Czajkowski S M | 2006 | J Cardiopulm Rehabil2006，26，1： | 1 |
| 9 | Ectopic preg- nancies in a caesarean scar: review of the medical approach to an iatrogenic complication显示文摘 | Mayrnon R Halperin R Mend|ovic S eta| | 2004 | Hum Reprod Update2004，10，： | 1 |
| 10 | The main benefits associated with health and safety management systems certification in Portuguese small and medium enterprises post quality management system certification 显示文摘 | Gilberto Santos S i ria Barros Fatima Mendes Nuno Lopes | 2013 | Safety Science2013，，51： | 1 |
| 11 | Evolution of networks显示文摘 | S N Dorogovtsev J F F Mendes | 2002 | Adv Phys2002，51，： | 1 |
| 12 | Ectopic micronodular thymoma with lymphoid stroma显示文摘 | Mende S Moschopulos M Marx A | 2004 | Virchows Arch2004，444，4： | 1 |
| 13 | Oncogenic transformation induced by membrane - targeted Akt2 and Akt3 显示文摘 | Mende I Malstrom S Tsichlis PN | 2001 | Oneogene2001，20，32： | 1 |
| 14 | Obstruction of pipelines due to paraffin deposition during the flow of crude oils显示文摘 | Ribeiro F S Mendes P R S Braga S L | 1997 | International Journal of Heat Mass Transfer1997，18，1： | 1 |
| 15 | Rolling - circle amplification of Torque teno virus ( TTV ) complete genomes from human and swine sera and identification of a novel swine TTV genogroup 显示文摘 | NIEL C DINIZ - MENDES L DEVALLE S | 2005 | J Virol2005，86，： | 1 |
| 16 | Structure of growing networks with preferential linking显示文摘 | Dorogovtsev S N Mendes J F F Samukhin A N | 2000 | Phys Rev Lett2000，85，21： | 1 |
| 17 | Determination of ancestral proportions in synthetic bovine breeds using commonly employed microsatellite markers显示文摘 | Bicalho H M S C G Pimenta I K P Mendes | 2006 | Genet Mol Res2006，5，3： | 1 |
| 18 | Human placenta is a potent hematopoietic niche containing hematopoietic stem and progenitor cells throughout development显示文摘 | Robin C Bollerot K Mendes S | 2009 | Cell Stem Cell2009，5，4： | 1 |
| 19 | Effects of the glyphosate-resistance gene and herbicides on soybean: Field trials monitoring biological nitrogen fixation and yield显示文摘 | Hungria M Mendes I C Nakatani A S | 2014 | Field Crops Research2014，158，： | 1 |
| 20 | Pseudofractal scale-free web显示文摘 | DOROGOVTSEV S N GOLTSEV A V MENDES J F F | 2002 | Phys Rev E2002，65，： | 1 |
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