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| 1 | Relationship between Fusobacterium nucleatum,inflammatory mediators and microRNAs in colorectal carcinogenesis显示文摘AIM To examine the effect of Fusobacterium nucleatum(F. nucleatum) on the microenvironment of colonic neoplasms and the expression of inflammatory mediators and microRNAs(miRNAs).METHODS Levels of F. nucleatum DNA, cytokine gene mRNA(TLR2, TLR4, NFKB1, TNF, IL1 B, IL6 and IL8), and potentially interacting miRNAs(miR-21-3p, miR-22-3p, mi R-28-5p, miR-34a-5p, miR-135b-5p) were measured by quantitative polymerase chain reaction(qPCR) TaqMan? assays in DNA and/or RNA extracted from the disease and adjacent normal fresh tissues of 27 colorectal adenoma(CRA) and 43 colorectal cancer(CRC) patients. KRAS mutations were detected by direct sequencing and microsatellite instability(MSI) status by multiplex PCR. Cytoscape v3.1.1 was used to construct the postulated miRNA:mRNA interaction network.RESULTS Overabundance of F. nucleatum in neoplastic tissue compared to matched normal tissue was detected in CRA(51.8%) and more markedly in CRC(72.1%). We observed significantly greater expression of TLR4, IL1 B, IL8, and miR-135 b in CRA lesions and TLR2, IL1 B, IL6, IL8, mi R-34 a and miR-135 b in CRC tumours compared to their respective normal tissues. Only two transcripts for miR-22 and miR-28 were exclusively downregulated in CRC tumour samples. The mRNA expression of IL1 B, IL6, IL8 and miR-22 was positively correlated with F. nucleatum quantification in CRC tumours. The mRNA expression of miR-135 b and TNF was inversely correlated. The miRNA:mRNA interaction network suggested that the upregulation of miR-34 a in CRC proceeds via a TLR2/TLR4-dependent response to F. nucleatum. Finally, KRAS mutations were more frequently observed in CRC samples infected with F. nucleatum and were associated with greater expression of miR-21 in CRA, while IL8 was upregulated in MSI-high CRC.CONCLUSION Our findings indicate that F. nucleatum is a risk factor for CRC by increasing the expression of inflammatory mediators through a possible mi RNA-mediated activation of TLR2/TLR4. | Marcela Alcantara Proenca Joice Matos Biselli Maysa Succi Fábio Eduardo Severino Gustavo Noriz Berardinelli Alaor Caetano Rui Manuel Reis David J Hughes Ana Elizabete Silva | 2018 | World Journal of Gastroenterology2018，24，47： | 15 |
| 2 | Validation of an immunoperoxidase monolayer assay for total anti-Vac- cinia virus antibody titration 显示文摘 | Gerber P F Matos A C Guedes M I | 2012 | J Vet Diagn Invest2012，24，2： | 1 |
| 3 | Central- to- peripheral fat ratio, but not peripheral body fat, is related to insulin resistance and androgen markers in polycystic ovary syndrome显示文摘 | Godoy- Matos AF Vaisman F Pedrosa AP | 2009 | Gynecol Endocrinol2009，25，12： | 1 |
| 4 | Competitive adsorption of zinc,cadmium,copper and lead in three highly weathered Brazilian soils显示文摘 | Fontes M P F Matos A F Costa L M | 2000 | Commun Soil Sci Plant Anal2000，31，： | 1 |
| 5 | Influence of limestone addition on the behavior of NO and N20 dur- ing fluidised bed coal combustion 显示文摘 | Tarelho L A C Matos M A A Pereira F J M A | 2006 | Fuel2006，85，7： | 1 |
| 6 | Diagenesis and reservoir quality evolution of fluvial sandstones during progressive burial and uplift : evi- dence from the Upper Jurassic Boipeba Member, Recrncavo basin, Northeastern Brazil显示文摘 | Salem A M Morad S Mato L F | 2000 | AAPG Bulletin2000，84，7： | 1 |
| 7 | Antineoplastic activity of selected constituents of Duguetia glabriuscula 显示文摘 | Matos M F Leite L I Brustolim D | 2006 | Fitoterapia2006，77，3： | 1 |
| 8 | Usefulness of HBME - 1, cytokeratin 19 and galectin - 3 immunostaining in the diagnosis of thyroid malignancy 显示文摘 | De matos PS Ferreira AP de Oliveira Facuri F | 2005 | Histopathology2005，47，4： | 1 |
| 9 | Aggressive angiomyxoma of vulva显示文摘 | Jardin O Matos R Falcao F | 2001 | Acta Med Port2001，14，： | 1 |
| 10 | Stimulation of Glutathione Synthesis of in Vitro Matured Bovine Oocytes and its Effect on Embryo Development and Freezability 显示文摘 | de Matos D G Furnus C C Moses D F | 1996 | M ol Reprod Dev1996，45，： | 1 |
| 11 | A review of the analytical methods to determine organic acids in grape juices and wines显示文摘 | Ines Mato Silvia Suarez-Luque Jose F Huidobro | 2005 | Food Research International2005，38，： | 1 |
| 12 | Tumor thickness as a predictive factor of lymph node metastasis and disease recurrence in T1N0 and T2N0 squamous cell carcinoma of the oral tongue显示文摘 | Leandro Luongo de Matos Gabriel Manfro Ricardo Vieira dos Santos Elaine Stabenow Evandro Sobroza de Mello Venancio Avancini F. Alves Fábio Roberto Pinto Marco Aurélio Vamondes Kulcsar Lenine Garcia Brand?o Cláudio Roberto Cernea | 2014 | Oral Surgery Oral Medicine Oral Pathology and Oral Radiology2014，，： | 1 |
| 13 | The effects of sleep deprivation on microRNA expression in rats submitted to pilocarpine-induced status epileptieus 显示文摘 | Matos G Scorza F A Mazzotti D R | 2014 | Prog Neuropsychopharmacol Biol Psychiatry2014，51，1： | 1 |
| 14 | Effect of sodium dodecylsulfate and benzotriazole on the interracial behavior of Cu/Cu(Ⅱ), H2SO4显示文摘 | Villamil R F V Cordeiro G G O Matos J | 2002 | Materials Chemistry and Physics2002，，78： | 1 |
| 15 | Effect of Cysteamine on Glutathione Level and Developmental Capacity of Bovine Oocyte Matured in Vitro显示文摘 | de Matos D G Furnus C C Moses D F | 1995 | Mol Reprod Dev1995，42，： | 1 |
| 16 | An improved RTN continuous-time formulation for the short-term scheduling of multipurpose batch plants 显示文摘 | CASTRO P BARBOSA-Povoa A P F D MATOS H | 2001 | Industrial and Engineering Chemistry Research2001，40，9： | 1 |
| 17 | Variable number tandem repeat loci providing discrimination within widespread genotypesof Acinetobacter baumannii 显示文摘 | Turton J F Matos J Kaufmann M E | 2009 | Eur J Clin Microbiol Infect Dis2009，28，5： | 1 |
| 18 | Midline omphalovesical anomalies inchildren: contribution of ultrasound imaging显示文摘 | Avni EF Matos C Diard F | 1988 | Urol Radiol1988，10，4： | 1 |
| 19 | Opposite effects of low versus high dose haloperidol treatments on spontaneous and apomorphine induced motor behavior:evidence that at a very low dose haloperidol acts as an indirect dopamine agonist显示文摘 | Dias FR de Matos LW Sampaio Mde F | 2012 | Behav Brain Res2012，229，1： | 1 |
| 20 | The electrochemical oxidation of xylitol on Pt(Ⅲ) in acid medium显示文摘 | MATOS J P F PROENCA L LOPES M I S | 2004 | J Electroanal Chem2004，571，： | 1 |
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