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| 1 | New gene therapy strategies for hepatic fibrosis显示文摘The liver is the largest internal organ of the body, which may suffer acute or chronic injury induced by many factors, leading to cirrhosis and hepatocarcinoma. Cirrhosis is the irreversible end result of fibrous scarring and hepatocellular regeneration, characterized by diffuse disorganization of the normal hepatic structure, regenerative nodules and fibrotic tissue. Cirrhosis is associated with a high co-morbidity and mortality without effective treatment, and much research has been aimed at developing new therapeutic strategies to guarantee recovery. Liver-based gene therapy has been used to downregulate specific genes, to block the expression of deleterious genes, to delivery therapeutic genes, to prevent allograft rejection and to augment liver regeneration. Viral and non-viral vectors have been used, with viral vectors proving to be more efficient. This review provides an overview of the main strategies used in liver-gene therapy represented by non-viral vectors, viral vectors, novel administration methods like hydrodynamic injection, hybrids of two viral vectors and blocking molecules, with the hope of translating findings from the laboratory to the patient′s bed-side. | Adriana M Salazar-Montes Luis D Hernández-Ortega Martha S Lucano-Landeros Juan Armendariz-Borunda | 2015 | World Journal of Gastroenterology2015，21，13： | 12 |
| 2 | Protective effects of anti-ricin A-chain RNA aptamer against ricin toxicity显示文摘AIM: To investigate the therapeutic potential of an RNA ligand (aptamer) specific for the catalytic ricin A-chain (RTA), the protective effects of a 31-nucleo-tide RNA aptamer (31RA), which formed a high affi nity complex with RTA, against ricin-induced toxicity in cellbased luciferase translation and cell cytotoxicity assays were evaluated. METHODS: To test the therapeutic potential of anti-RTA aptamers in Chinese hamster ovary (CHO) AA8 cells stably transfected with a tetracycline regulat-able promoter, ricin ribotoxicity was measured using luciferase and ricin-induced cytotoxicity was ascertained by MTS cell proliferation assay with tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium]. RESULTS: Inhibition of protein synthesis by ricin in CHO AA8 cells resulted in diminished luciferase activity and treatment with polyclonal antibody against degly-cosylated RTA (dgA) neutralized the inhibitory effects of ricin on luciferase activity and protected against ricin-induced cytotoxicity as measured by MTS assay. The 31RA anti-RTA aptamer inhibited the translation of luciferase mRNA in cell-free reticulocyte translation as- say. 31RA aptamer also partially neutralized the inhibitory effects of ricin on luciferase activity and partially protected against ricin-induced cytotoxicity in CHO AA8 cells. CONCLUSION: We have shown that anti-RTA RNA aptamer can protect against ricin ribotoxicity in cellbased luciferase and cell cytotoxicity assays. Hence, RNA aptamer that inhibits RTA enzymatic activity represents a novel class of nucleic acid inhibitor that has the potential to be developed as a therapeutic agent for the treatment of ricin intoxication. | Shaoan Fan Feng Wu Frank Martiniuk Martha L Hale Andrew D Ellington Kam-Meng Tchou-Wong | 2008 | World Journal of Gastroenterology2008，14，41： | 3 |
| 3 | Starch synthesis in the cereal endosperm显示文摘 | MARTHA G J KAY D ALAN M M | 2003 | Current Opinion in Plant Biology2003，6，： | 1 |
| 4 | Mutational analysis of the pullulanase-type debranching enzyme of maize indicates multiple functions in starch metabolism 显示文摘 | Jason R D Christophe C Martha G J | 2003 | Plant Cell2003，15，： | 1 |
| 5 | The response ot sod ero- sion and sediment export to land-use change in four areas of Europe : the importance of landscape pattern 显示文摘 | Martha M B Gerard G Anne V D | 2008 | Geomorphology2008，98，34： | 1 |
| 6 | Global leaders are team players: developing global leaders through membership on global teams 显示文摘 | Martha L M Joseph J D | 2000 | Human Resource Management2000，39，23： | 1 |
| 7 | Halogenation of 2-Substituted-4- isothiazolin-3-ones显示文摘 | Ernest D Weiler Ramesh B Petogara Martha H Wolfersberger | 1977 | Journal of Heterocyelie Chem- istry1977，14，： | 1 |
| 8 | Supply chain Management: More Than a New name for Logistics 显示文摘 | Cooper Martha C Douglas M Lambert Janus D pagh | 1997 | The International Journal of Operations and Production Management1997，88，1： | 1 |
| 9 | A rod end deletion in the intermediate filament protein nestin alters its subcellular localization in neuroepithelial cells of transgenic mice显示文摘 | Martha JM Jonas D Urban L | 1998 | J Cell Science1998，111，14： | 1 |
| 10 | Biomarkers of brain injury in the premature infan-t 显示文摘 | Martha Douglas-Escobar Michael D Weiss | 2013 | Review Article2013，3，185： | 1 |
| 11 | Longitudinal associations between blood lead concentrations lower than 10μg/dL and neurobehavioral development in environmentally exposed children in Mexico City 显示文摘 | Martha M T Sc D David C | 2006 | Pediatrics2006，118，2： | 1 |
| 12 | Observation of stereochemical configuration of poly (methyl mathacrylate) by proton two-dimentional J-correlated and NOE-correlated NMR spectroscopy 显示文摘 | Fredric C S Frank A B Martha D B | 1985 | Macromolecules1985，18，7： | 1 |
| 13 | Polymorphic variation of the guanosine triphosphate cyclo- hydrolase 1 gene predicts outcome in patients undergoing surgi-cal treatment for lumbar degenerative disc disease 显示文摘 | Kim D H Dai F Belfer I Banco R J Martha J F Tighiouart H | 2010 | Spine (Phila Pa 1976)2010，35，： | 1 |
| 14 | An analysis of genetic factors related to risk of inflammatory bowel disease and colon cancer显示文摘 | Bríd M. Ryan Roger K. Wolff Nicola Valeri Mohammed Khan Dillon Robinson Alessio Paone Elise D. Bowman Abbie Lundgreen Bette Caan John Potter Derek Brown Carlo Croce Martha L. Slattery Curtis C. Harris | 2014 | Cancer Epidemiology2014，，5： | 1 |
| 15 | Efficient hydrogen production ly composite photocatalyst CdS-ZnS/Zirconiume- titanium phosphate ( ZTP ) under visible light illumination 显示文摘 | BISWAL N DAS D P MARTHA S | 2011 | International Journal of Hydrogen Energy2011，36，13： | 1 |
| 16 | Starch synthesis in the cereal endosperm显示文摘 | Martha G J Kay D Alan M M | 2003 | Curr Opin Plant Biol2003，6，3： | 1 |
| 17 | Associations between Recent Exposure to Ambient Fine Particulate Matter and Blood Pressure in the Multi-Ethnic Study of Atherosclerosis (MESA)显示文摘 | Auchincloss Amy H Roux Ana V Diez Dvonch J Timothy Brown Patrick L Barr R Graham Daviglus Martha L Goff David C Kaufman Joel D O’Neill Marie S | 2008 | Environmental Health Perspectives2008，，4： | 1 |
| 18 | Glycosaminoglycan in articular cartilage:in vivo assessment with delayed GD (DTPA)2- enhanced MR imaging显示文摘 | Martha L Robert D | 1997 | Rdiology1997，205，： | 1 |
| 19 | Starch synthesis in the cereal endosperm显示文摘 | Martha G J Kay D Alan M M | 2003 | Current Opinion in Plant biology2003，6，： | 1 |
| 20 | Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines显示文摘 | Ricardo Vera-Bravo Elizabeth Torres John J. Valbuena Marisol Ocampo Luis E. Rodríguez álvaro Puentes Javier E. García Hernando Curtidor Jimena Cortés Magnolia Vanegas Zuly J. Rivera Andrea Díaz Martha N. Calderon Manuel A. Patarroyo Manuel E. Patarroyo | 2005 | Biochemical and Biophysical Research Communications2005，，： | 1 |
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