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| 1 | Significant decrease in prevalence ofHelicobacter pylori in the Czech Republic显示文摘AIM:To study possible decrease in prevalence of Helicobacter pylori(H.pylori) infection in the Czech Republic within a 10-year period.METHODS:A total of 22 centres entered the study.The catchment areas of these centres covered cities and towns with more than 20 000 inhabitants,smaller towns(≤ 20 000 inhabitants) with surrounding villages and rural areas,and were spread over the whole country,corresponding well to the geographical distribution of the Czech population.A total of 1 837 subjects(aged 5-98 years) took part in the study,randomly selected out of 38 147 people from the general population.H.pylori infection was investigated by means of a 13 C-urea breath test.Breath samples in duplicates were analysed using isotope ratio mass spectrometry.The cut-off point was 3.5.Social and demographic characteristics were based on data from self-completed questionnaires.RESULTS:The overall prevalence of H.pylori infection was 23.5%(430/1826),and 4.8%(20/420) in children aged 15 or less.There was no statistically significant difference in prevalence between males(24.3%;208/857) and females(22.9%,222/969,P = 0.494).H.pylori infection was strongly associated with higher age,among subjects aged 55+ years,prevalence of H.pylori infection was 39.8%(252/633,P < 0.001).The highest prevalence of H.pylori infection was found among persons aged 55-64 years(43.9%,97/221) and 75+ years(37.9%,58/153).Among study subjects aged 15+ years,prevalence of H.pylori infection was significantly increased in those with lowest education(odds risk 3.19,95% CI 1.87-5.47).Compared to never married(14.1%),the prevalence of H.pylori infection was statistically significantly higher among married(35.4%,246/694,P < 0.001),divorced(36.8%,49/133,P < 0.001) and widowed study subjects(40.2%,45/112,P < 0.001),both in minimally and fully adjusted analysis.There was no significant difference in the prevalence of H.pylori infection between married and widowed subjects(35.4%,246/694 vs 40.2%,45/112,P = 0.389).There was little variation in smoking prevalence across categories of smoking and there was no evidence of an increased risk ofH.pylori infection among current or past smokers in our data(odds risk 1.04 with 95% CI 0.78-1.40 for current smokers;odds ratio 0.83 with 95% CI 0.60-1.16 for former smokers).The current prevalence of H.pylori in 2011 was significantly lower compared to the prevalence reported from identical geographical areas in 2001(23.5%vs 41.7%,P < 0.001).CONCLUSION:The overall prevalence of H.pylori infection in the general population has fallen substantially in the Czech Republic over the past 10 years. | Jan Bure Marcela Kopáová Ilona Koupil Bohumil Seifert Miluka kodová Fendrichová Jana pirková Viktor Voíek Stanislav Rejchrt TomáDouda Norbert Král Ilja Tachecí | 2012 | World Journal of Gastroenterology2012，18，32： | 8 |
| 2 | Reallocation of foliicolous species of the genus Strigula into six genera(lichenized Ascomycota,Dothideomycetes,Strigulaceae)显示文摘Strigula sensu lato has been previously defined based on phenotype characters as a rather broad genus including tropical to temperate species growing on a wide array of substrata.In this study,based on a multilocus phylogenetic approach,we show that foliicolous species form six well-delimited clades that correlate with diagnostic phenotype features,including thallus morphology,carbonization of the involucrellum and excipulum,ascospore dimensions,and type of macroconidia.Given the topology,with five of the six clades emerging on long stem branches,and the strong phenotypical differentiation between the clades,we recognize these at the genus level,making mostly use of previously established genus names.Four genera,namely Phylloporis,Puiggariella,Raciborskiella,and Racoplaca,are resurrected for the S.phyllogena,S.nemathora,S.janeirensis,and S.subtilissima groups,respectively,whereas one new genus,Serusiauxiella gen.nov.,is introduced for a novel lineage with peculiar macroconidia.The only sequenced non-foliicolous species,S.jamesii,is not closely related to these six foliicolous lineages but clusters with Flavobathelium and Phyllobathelium,revealing it as an additional undescribed genus-level lineage being treated elsewhere.Within the new genus Serusiauxiella,three new species are described:Serusiauxiella filifera sp.nov.,S.flagellata sp.nov.,and S.sinensis sp.nov.In addition,ten new combinations are proposed:Phylloporis austropunctata comb.nov.,P.radiata comb.nov.,P.vulgaris comb.nov.,Puiggariella confluens comb.et stat.nov.,P.nemathora comb.nov.,P.nigrocincta comb.nov.,Racoplaca maculata comb.nov.,R.melanobapha comb.nov.,R.transversoundulata,and R.tremens comb.nov.We also report on a peculiar,previously unrecognized growth behaviour of the macroconidial appendages in Strigula s.lat. | Shu‑Hua Jiang Robert Lücking Amanda Barreto Xavier‑Leite Marcela E.S.Cáceres AndréAptroot Carlos Vinas Portilla Jiang‑Chun Wei | 2020 | Fungal Diversity2020，，3： | 5 |
| 3 | Effect of natural and synthetic antioxidants on the oxidative stability of walnut oil under different storage conditions 显示文摘 | Marcela L Martinez Ma Cecilia Penci Vanesa Ixtaina | 2013 | LWT - Food Science and Technology2013，51，： | 1 |
| 4 | Permanent edu- cation in BLS and ACLS: impact on the knowledge of nursing professionals显示文摘 | Gonalves S Araripe L Marcela M | 2009 | Arq Bras Cardiol2009，93，6： | 1 |
| 5 | Analysis of heat transfer in a vessel with helical pipe coil and multistage impeller 显示文摘 | Martin Dostál Marcela Vě?í?ová Karel Petera Tomá? Jirout Ivan Fo?t | 2014 | The Canadian Journal of Chemical Engineering2014，92，： | 1 |
| 6 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
| 7 | Pancreatic insulin secretion in rats fed a soy protein high fat diet depends on the interaction between the amino acid pattern and isoflavones显示文摘 | Lilia N L Armando R T Marcela G G | 2007 | J Biol Chem2007，282，20： | 1 |
| 8 | A high-salt diet stimulates thick ascending limb eNOS expression by raising medullary osmolality and increasing release of endothelin-1显示文摘 | Marcela Herrera Jeffrey L Garvin | 2005 | Am J Physiol Renal Physiol2005，288，： | 1 |
| 9 | Oriented immobilization of galactose oxidase to bead and magnetic bead cellulose and poly (HEMA-co-EDMA) and magnetic poly(HEMA-co-EDMA) microspheres显示文摘 | Zuzana B Marcela S Antonin L | 2002 | Journal of Chromatography B2002，770，12： | 1 |
| 10 | Epigenetic inactivation of EGFR by CpG island hypermethylation in cancer 显示文摘 | Montero A J Marcela Dlaz - Montero C Mao L | 2006 | Cancer Biol Ther2006，5，： | 1 |
| 11 | The use of flow cytometry in the evaluation of cell viability of cryopreserved sperm of the marine shrimp(Litopenaeus vannamei ) 显示文摘 | Magda L Clarissa G Marcela S | 2004 | Cryobiology2004，48，3： | 1 |
| 12 | Bioavailability of microencapsulated ferrous sulfate in powdered milk produced from fortified fluid milk:A prophylactic study in rats显示文摘 | Alexis E L Marcela B Z Maria J S | 2002 | Nutrition2002，18，3： | 1 |
| 13 | Dissolving pulp production from sugar cane bagasse显示文摘 | Marcela F A Jorge L C | 2014 | Industrial Crops and Products2014，53，： | 1 |
| 14 | The Role of Astrocytes and Complement System in Neural Plasticity显示文摘 | Milos Pekny Ulrika Wilhelmsson Yalda Rahpeymai Bogest?l Marcela Pekna | 2007 | International Review of Neurobiology2007，，： | 1 |
| 15 | Histone deacetylase inhibitor prevents cell growth in Burkitt’s lymphoma by regulating PI3K/Akt pathways and leads to upregulation of miR-143, miR-145, and miR-101显示文摘 | Ana Carolina Santos Ferreira Marcela Cristina Robaina Lídia Maria Magalh?es Rezende Patricia Severino Claudete Esteves Klumb | 2014 | Annals of Hematology2014，，6： | 1 |
| 16 | Questionnaire-based second-hand smoke assessment in adults显示文摘 | Mó nica Pé rez-Rí os Anna Schiaffino Marí a José Ló pez Manel Nebot I? aki Galá n Marcela Fu José Marí a Martí nez-Sá nchez Albert Moncada Agustí n Montes Carles Ariza Esteve Ferná ndez | 2013 | The European Journal of Public Health2013，，5： | 1 |
| 17 | Interlenticular opacification: clinicopathological correlation of a complication of posterior chamber piggyback intraocular lenses显示文摘 | Johnny L Gayton David J Apple Qun Peng Nithi Visessook Val Sanders Liliana Werner Suresh K Pandey Marcela Escobar-Gomez Daphne S.M Hoddinott Michelle Van Der Karr | 2000 | Journal of Cataract & Refractive Surgery2000，，3： | 1 |
| 18 | Fungal diversity notes 253-366:taxonomic and phylogenetic contributions to fungal taxa显示文摘Notes on 113 fungal taxa are compiled in this paper,including 11 new genera,89 new species,one new subspecies,three new combinations and seven reference specimens.Awide geographic and taxonomic range of fungal taxa are detailed.In the Ascomycota the new genera Angustospora(Testudinaceae),Camporesia(Xylariaceae),Clematidis,Crassiparies(Pleosporales genera incertae sedis),Farasanispora,Longiostiolum(Pleosporales genera incertae sedis),Multilocularia(Parabambusicolaceae),Neophaeocryptopus(Dothideaceae),Parameliola(Pleosporales genera incertae sedis),and Towyspora(Lentitheciaceae)are introduced.Newly introduced species are Angustospora nilensis,Aniptodera aquibella,Annulohypoxylon albidiscum,Astrocystis thailandica,Camporesia sambuci,Clematidis italica,Colletotrichum menispermi,C.quinquefoliae,Comoclathris pimpinellae,Crassiparies quadrisporus,Cytospora salicicola,Diatrype thailandica,Dothiorella rhamni,Durotheca macrostroma,Farasanispora avicenniae,Halorosellinia rhizophorae,Humicola koreana,Hypoxylon lilloi,Kirschsteiniothelia tectonae,Lindgomyces okinawaensis,Longiostiolum tectonae,Lophiostoma pseudoarmatisporum,Moelleriella phukhiaoensis,M.pongdueatensis,Mucoharknessia anthoxanthi,Multilocularia bambusae,Multiseptospora thysanolaenae,Neophaeocryptopus cytisi,Ocellularia arachchigei,O.ratnapurensis,Ochronectria thailandica,Ophiocordyceps karstii,Parameliola acaciae,P.dimocarpi,Parastagonospora cumpignensis,Pseudodidymosphaeria phlei,Polyplosphaeria thailandica,Pseudolachnella brevifusiformis,Psiloglonium macrosporum,Rhabdodiscus albodenticulatus,Rosellinia chiangmaiensis,Saccothecium rubi,Seimatosporium pseudocornii,S.pseudorosae,Sigarispora ononidis and Towyspora aestuari.New combinations are provided for Eutiarosporella dactylidis(sexual morph described and illus trated)and Pseudocamarosporium pini.Descriptions,illustrations and/or reference specimens are designated for Aposphaeria corallinolutea,Cryptovalsa ampelina,Dothiorella vidmadera,Ophiocordyceps formosana,Petrakia echinata,Phragmoporthe conformis and Pseudocamarosporium pini.The new species of Basidiomycota are Agaricus coccyginus,A.luteofibrillosus,Amanita atrobrunnea,A.digitosa,A.gleocystidiosa,A.pyriformis,A.strobilipes,Bondarzewia tibetica,Cortinarius albosericeus,C.badioflavidus,C.dentigratus,C.duboisensis,C.fragrantissimus,C.roseobasilis,C.vinaceobrunneus,C.vinaceogrisescens,C.wahkiacus,Cyanoboletus hymenoglutinosus,Fomitiporia atlantica,F.subtilissima,Ganoderma wuzhishanensis,Inonotus shoreicola,Lactifluus armeniacus,L.ramipilosus,Leccinum indoaurantiacum,Musumecia alpina,M.sardoa,Russula amethystina subp.tengii and R.wangii are introduced.Descriptions,illustrations,notes and/or reference specimens are designated for Clarkeinda trachodes,Dentocorticium ussuricum,Galzinia longibasidia,Lentinus stuppeus and Leptocorticium tenellum.The other new genera,species new combinations are Anaeromyces robustus,Neocallimastix californiae and Piromyces finnis from Neocallimastigomycota,Phytophthora estuarina,P.rhizophorae,Salispina,S.intermedia,S.lobata and S.spinosa from Oomycota,and Absidia stercoraria,Gongronella orasabula,Mortierella calciphila,Mucor caatinguensis,M.koreanus,M.merdicola and Rhizopus koreanus in Zygomycota. | Guo Jie Li Kevin D.Hyde Rui Lin Zhao Sinang Hongsanan Faten Awad Abdel-Aziz Mohamed A.Abdel-Wahab Pablo Alvarado Genivaldo Alves-Silva Joseph F.Ammirati Hiran A.Ariyawansa Abhishek Baghela Ali Hassan Bahkali Michael Beug D.Jayarama Bhat Dimitar Bojantchev Thitiya Boonpratuang Timur S.Bulgakov Erio Camporesi Marcela CBoro Oldriska Ceska Dyutiparna Chakraborty Jia Jia Chen K.W.Thilini Chethana Putarak Chomnunti Giovanni Consiglio Bao Kai Cui Dong Qin Dai Yu Cheng Dai Dinushani A.Daranagama Kanad Das Monika C.Dayarathne Eske De Crop Rafael J.V.De Oliveira Carlos Alberto Fragoso de Souza JoséIde Souza Bryn T.M.Dentinger Asha J.Dissanayake Mingkwan Doilom E.Ricardo Drechsler-Santos Masoomeh Ghobad-Nejhad Sean P.Gilmore Aristóteles Góes-Neto MichałGorczak Charles H.Haitjema Kalani Kanchana Hapuarachchi Akira Hashimoto Mao Qiang He John K.Henske Kazuyuki Hirayama Maria J.Iribarren Subashini C.Jayasiri Ruvishika S.Jayawardena Sun Jeong Jeon Gustavo H.Jerônimo Ana L.Jesus E.B.Gareth Jones Ji Chuan Kang Samantha C.Karunarathna Paul M.Kirk Sirinapa Konta Eric Kuhnert Ewald Langer Haeng Sub Lee Hyang Burm Lee Wen Jing Li Xing Hong Li Kare Liimatainen Diogo Xavier Lima Chuan Gen Lin Jian Kui Liu Xings Zhong Liu Zuo Yi Liu J.Jennifer Luangsa-ard Robert Lücking H.Thorsten Lumbsch Saisamorn Lumyong Eduardo M.Leaño Agostina V.Marano Misato Matsumura Eric H.C.McKenzie Suchada Mongkolsamrit Peter E.Mortimer Thi Thuong Thuong Nguyen Tuula Niskanen Chada Norphanphoun Michelle A.O’Malley Sittiporn Parnmen Julia Pawłowska Rekhani H.Perera Rungtiwa Phookamsak Chayanard Phukhamsakda Carmen L.A.Pires-Zottarelli Olivier Raspé Mateus A.Reck Sarah C.O.Rocha AndréL.C.M.Ade Santiago Indunil C.Senanayake Ledo Setti Qiu Ju Shang Sanjay K.Singh Esteban B.Sir Kevin V.Solomon Jie Song Prasert Srikitikulchai Marc Stadler Satinee Suetrong Hayato Takahashi Takumasa Takahashi Kazuaki Tanaka Li Ping Tang Kasun M.Thambugala Donnaya Thanakitpipattana Michael K.Theodorou Benjarong Thongbai Tuksaporn Thummarukcharoen Qing Tian Saowaluck Tibpromma Annemieke Verbeken Alfredo Vizzini Josef Vlasák Kerstin Voigt Dhanushka N.Wanasinghe Yong Wang Gothamie Weerakoon Hua An Wen Ting Chi Wen Nalin N.Wijayawardene Sarunyou Wongkanoun Marta Wrzosek Yuan Pin Xiao Jian Chu Xu Ji Ye Yan Jing Yang Shu Da Yang Yu Hu Jin Feng Zhang Jie Zhao Li Wei Zhou Derek Peršoh Alan J.L.Phillips Sajeewa S.N.Maharachchikumbura | 2016 | Fungal Diversity2016，，3： | 1 |
| 19 | Using niche-based GIS modeling to test geographic predictions of competitive exclusion and competitive release in South American pocket mice显示文摘 | Anderson R P Peterson A T Marcela G L | 2002 | OIKOS2002(98) : 3 - 162002，2002，98： | 1 |
| 20 | Char- acterization of chia seed oils obtained by pressing and solvent ex- traction显示文摘 | Vanesa Y Ixtaina Marcela L Martinez Viviana Spotorno | 2011 | Journal of Food Composition and Analysis2011，24，2： | 1 |
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