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| 1 | A next-generation marker genotyping platform(AmpSeq)in heterozygous crops:a case study for marker-assisted selection in grapevine显示文摘Marker-assisted selection(MAS)is often employed in crop breeding programs to accelerate and enhance cultivar development,via selection during the juvenile phase and parental selection prior to crossing.Next-generation sequencing and its derivative technologies have been used for genome-wide molecular marker discovery.To bridge the gap between marker development and MAS implementation,this study developed a novel practical strategy with a semi-automated pipeline that incorporates trait-associated single nucleotide polymorphism marker discovery,low-cost genotyping through amplicon sequencing(AmpSeq)and decision making.The results document the development of a MAS package derived from genotyping-by-sequencing using three traits(flower sex,disease resistance and acylated anthocyanins)in grapevine breeding.The vast majority of sequence reads(⩾99%)were from the targeted regions.Across 380 individuals and up to 31 amplicons sequenced in each lane of MiSeq data,most amplicons(83 to 87%)had<10%missing data,and read depth had a median of 220–244×.Several strengths of the AmpSeq platform that make this approach of broad interest in diverse crop species include accuracy,flexibility,speed,high-throughput,low-cost and easily automated analysis. | Shanshan Yang Jonathan Fresnedo-Ramíre Minghui Wang Linda Cote Peter Schweitzer Paola Barba Elizabeth M Takacs Matthew Clark James Luby David C Manns Gavin Sacks Anna Katharine Mansfield Jason Londo Anne Fennell David Gadoury Bruce Reisch Lance Cadle-Davidson Qi Sun | 2016 | Horticulture Research2016，3，1： | 2 |
| 2 | Functional magnetic resonance imaging in a visual oddball task显示文摘 | McCarthy G Luby M Gore JC | 1996 | Neuroimage1996，3，： | 2 |
| 3 | How to Construct Pseudo-Random Permutations from Pseudo-Random Functions显示文摘 | Luby M Rackoff C | 1988 | SIAM Journal on Computing1988，17，2： | 1 |
| 4 | Genotypic and environmental variation in antioxidant activity, total phenolic content, and anthocyanin content among blueberry cultivars显示文摘 | CONNOR A M LUBY J J TONG C B S | 2002 | Journal of American Society for Horticultural Science2002，127，1： | 1 |
| 5 | Distal hyperintense vessels on FLAIR:an MRI marker for collateral circulation in acute stroke显示文摘 | Lee KY Latour LL Luby M | | 0，，： | 1 |
| 6 | Effect of handwashing on child health: a randomised controlled trial显示文摘 | Luby SP Agboatwalla M Feikin DR | 2005 | Lancet2005，366，9481： | 1 |
| 7 | Efficient Erasure Correcting codes 显示文摘 | Luby M Mitzenmacher M Shokrollahi A | 2001 | IEEE Transactions on Information Theory2001，47，2： | 1 |
| 8 | Improved Low-Density Parity-Check Codes Using Irregular Graphs显示文摘 | Luby M G Mitzenmacher M Shokrollahi M A | 2001 | IEEE Transactions on Information Theory2001，47，2： | 1 |
| 9 | Efficient erasure correcting codes 显示文摘 | Luby M G Mitzenmacher M Shokrollahi M A | 2001 | IEEE Trans Info Theo2001，47，2： | 1 |
| 10 | Hemodynamic physiology and thermoregulation in liposuction显示文摘 | Kenkel JM Lipschitz AH Luby M | 2004 | Plast Reconstr Surg2004，114，2： | 1 |
| 11 | Distal hyperintense vessels on FLAIR:an MRI marker for collateral circulation in acute stroke显示文摘 | Lee KY Latour LL Luby M | | 0，，13： | 1 |
| 12 | Intermixing inimmiscible molybdenum/coppermultilayered metallization under excimer laser irradiation显示文摘 | S Luby E Majkova M Jergerl | 1996 | Applied SurfaceScience1996，106，： | 1 |
| 13 | A dig- ital fountain approach to asynchronous reliable mul- ticast显示文摘 | Byers J W Luby M Mitzenmacher M | 2002 | IEEE Journal on Selected Areas in Com-munications2002，20，8： | 1 |
| 14 | A bidirectional shortest-path algorithmwith good average-case behavior显示文摘 | LUBY M RAGDE P | 1989 | Algorithmica1989，4，14： | 1 |
| 15 | Approximating probabilistic inference in Bayesian belief networks is NP-hard 显示文摘 | Luby M | 1993 | Artificial Intelligence1993，60，： | 1 |
| 16 | Electrolyte and plasma enzyme analyses during large-volume liposuction 显示文摘 | Lipschitz AH Kenkel JM Luby M | 2004 | Plast Reconstr Surg2004，114，3： | 1 |
| 17 | Laser-flash photographic studies of Er:YAG laser ablation of water 显示文摘 | D'Anna E Luby S Luches A M | 1993 | Newsletter Physics1993，2，3： | 1 |
| 18 | Raptor codes显示文摘 | Shokrollahi A and Luby M | 2011 | Foundations and Trends in Communications and Information Theory2011，6，34： | 1 |
| 19 | Foodborne transmission of Nipah virus,Bangladesh显示文摘 | Rahman M Hossain M J | 2006 | Emerg Infect Dis2006，12，12： | 1 |
| 20 | Improved low-density paritycheck codes using irregular graphs显示文摘 | Luby M G Mitzenmacher M | 2001 | IEEE Transactions on Information Theory2001，47，： | 1 |
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