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88篇 您的检索式:作者名="Kabin"
    题名 作者 年代 出处 被引量
1RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System显示文摘Kabin Xie Yinong Yang 2013Molecular Plant2013,6,6:98
2Genome-Wide Prediction of Highly Specific Guide RNA Spacers for CRISPR-Cas9-Mediated Genome Editing in Model Plants and Major Crops显示文摘Dear Editor, RNA-guided genome editing (RGE) using the Streptococcus pyogenes CRISPR-Cas9 system (Jinek et al.,2012;Cong et al.,2013;Mali et al.,2013b) is emerging as a simple and highly efficient tool for genome editing in many organisms.The Cas9 nuclease can be programmed by dual or single guide RNA (gRNA) to cut target DNA at specific sites,thereby introducing precise mutations by error-prone non-homologousend-joining repairing or by incorporating foreign DNAs via homologous recombination between target site and donor DNA.The gRNA-Cas9 complex recognizes targets based on the complementarity between one strand of targeted DNA (referred as protospacer) and the 5'-end leading sequence of gRNA (referred to as gRNA spacer) that is approximately 20 base pairs (bp) long (Figure 1A).Besides gRNA-DNA pairing,a protospacer-adjacent motif (PAM) following the paired region in the DNA is also required for Cas9 cleavage.Recent studies reveal that Cas9 could cut the PAM-containing DNA sites that imperfectly match gRNA spacer sequences,resulting in genome editing at undesired positions.Kabin Xie Jianwei Zhang Yinong Yang 2014Molecular Plant2014,7,5:35
33RISPR-P 2.0: An Improved CRISPR-Cas9 Too For Genome Editing in Plants显示文摘Hao Liu Yuduan Ding Yanqing Zhou Wenqi Jin Kabin Xie Ling-Ling Chen 2017Molecular Plant2017,10,3:32
4Engineering Introns to Express RNA Guides for Cas9- and Cpfl-Mediated Multiplex Genome Editing显示文摘定期聚类 interspaced 短 palindromic 重复(CRISPR ) 系统为指向的 DNA 作为革命站台出现了。这个系统使用一个地点特定的 RNA 指南指导一个 CRISPR 受动器(例如, Cas9 和 Cpf1 ) 到一个 DNA 目标。这里,我们详细描述一般策略同时从 Cas9 和 Cpf1 的 introns 表示多重指南 RNA (gRNA ) 和 CRISPR RNA (crRNA ) 。这个方法利用处理系统或 Cpf1 的 crRNA 处理活动劈开包含 tRNA-gRNA polycistron 或 crRNA-crRNA 数组的拼接的 intron 的内长的 tRNA。我们证明 tRNA-gRNA intron 能作为一基因与 Cas9 熔化。如此的混合基因能用一个聚合酶 II 倡导者被表示,并且在同时指向多重地点展出了高效率和坚韧性。我们 ? 也在调停 Cpf1 的染色体用 intronic tRNA-crRNA 和 crRNA-crRNA 数组编辑实现了这策略。有趣地,包含 Cpf1 和 intronic crRNA 的混合基因与常规 Cpf1 向量相比穿展出显著地增加的效率。一起拿,这研究介绍一个方法从一混合基因表示 CRISPR 试剂增加编辑染色体的效率和能力。由于它的简洁和通用性,这个方法能广泛地被用来在优核质开发复杂 CRISPR 工具。Dan Ding Kaiyuan Chen Yuedan Chen Hong Li Kabin Xie 2018Molecular Plant2018,11,4:14
5Psychology and Economics 显示文摘Matthew Kabin 1998Journal of Economic Literature American Economic Association1998,36,1:1
6Pedicle morphology in the upper thoracic spine: limits to safe screw placement in older patients 显示文摘McLain RF Ferrera L Kabins M 2002Spine2002,22,:1
7Isolated L4-5 fusions using the variable screw placement system:unilateral versus bilateral显示文摘Kabins MB Weintein JN Spratt KF 1992Spine1992,5,1:1
8Isolated L4-L5 fusions using the variable screw placement system: unilateral ver- sus bilateral显示文摘Kabins MB Weinstein JN Spratt KF 1992J Spinal Disord1992,5,1:1
9Isolated L4-L5 fusions using the variable screw placement system:unilateral versus bilateral显示文摘Kabins MB Weinstein JN Spratt KF 1992J Spinal Disord1992,5,1:1
10Isolated L4--5 fu- sions using the variable screw placement system:unilateralvenusbilateral显示文摘Kabins MB Weintein JN Spratt KF 1992Spine1992,5,1:1
11Isolated La-L5 fusions using the variable screw placement system: unilateral versus bilateral显示文摘Kabins MB Wdnstdn JN Spratt KF 1992J Spinal Disord1992,5,1:1
12Isolated L4 - L5 fusions u- sing the variable screw placement system: unilateral versus bilateral 显示文摘Kabins MB Weinstein JN Spratt KF 1992J Spinal Disord Tech1992,5,:1
13Isolated L4-L5 fusions using the variable screw placement system: unilateral versus bilateral 显示文摘Kabins MB Weinstein JN Spratt KF 1992J Spinal Dis- ord1992,5,1:1
14A novel approach toward pedicle screw placement in the thoracic spine显示文摘John Thalgott Mark Kabins James Giuffre 2002The Spine Journal2002,,5:1
15Isolated IA5 fusins using the variable screw placement system:unilateral versus bilatepai 显示文摘Kabins MB Weinstein JN Spratt KF 1992J Sprinal Disrd1992,5,:1
16Physiological,morphologicaland merabolic changes inTetrahyllena pyriK, rnlisKr the in vivo cytotoxiciW assessment of metallic pollution:Inlpact on D- ]3 -hy droxybutyrate dehy-drogenase显示文摘Mountassif D Kabine M Manar R 2007Ecological Indicators2007,7,4:1
17Isolated L4~sfusions using the variable screw placement system: unilateral versus bilateral 显示文摘Kabins MB Weinstein JN Spratt KF 1992J Spinal Disord Tech1992,5,1:1
18Isolated IA-L5 fusions using the variable screw placement system: unilateral versus bilateral显示文摘Kabins MB Weinstein JN Spratt KF 1992J Spinal Disord1992,5,1:1
19Isolated L4-5 fusions using the variable screw placement sysKabins MB,WeinteinJN,Stem:unilateral versus bilateral显示文摘Kabins MB Weintein JN Spratt KF 1992J Spine Disord1992,5,1:1
20Isolated L4 - L5 fusions using the variable screw placement system unilateral versus bilateral显示文摘Kabins MB Weinstein JN Spratt KF 1992J Spinal Disord1992,5,1:1
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