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| 1 | Surgical stress and cytoskeletal changes in lens epithelial cells following manual and femtosecond laser-assisted capsulotomy显示文摘AIM:To study the effect of mechanical stress on the cytoskeleton in lens epithelial cells following conventional phacoemulsification surgery(CPS)and femtosecond laserassisted cataract surgery(FLACS).METHODS:The cytoskeleton of the epithelial cells of the anterior lens capsules(ALC)removed by CPS and FLACS was examined by immunohistochemistry.Expression of the intermediate filament,glial fibrillary acidic protein(GFAP),and glutamine synthetase(GS)immunoreactivity were detected.In order to map the actin network of cells,fluorescently labeled phalloidin was used.The samples were examined using confocal laser scanning microscopy.RESULTS:GFAP expression was visible in a larger number of the epithelial cells after CPS compared to FLACS.In CPS sample’s epithelial cells,GFAP immunoreactivity indicated robust morphological change.Regarding the actin filaments,the presence of tubular elements connecting epithelial cells,regular actin pattern and marked cortical network after CPS were found.Following FLACS,the actin cytoskeleton of the epithelial cells remained densely structured,and the tubular elements were undetectable,however,the above-mentioned regular actin pattern and the marked cortical network were visible.CONCLUSION:The conventional removal of the ALC induces more robust changes of the cytoskeleton of the lens epithelial cells. | ANDrea Krisztina Sükosd Krisztina Szabadfi Edina Szabó-Meleg Beáta Gáspár Pavel Stodulka Gyorgy SétálóJr Róbert Gábriel Miklós Nyitrai Zsolt Biró Hajnalka Abrahám | 2020 | International Journal of Ophthalmology(English edition)2020，13，6： | 2 |
| 2 | Effects on reproduction performance of administration of GnRH to early postpartum dairy cows显示文摘 | Nash Jr J G Ball L Olson J D | 1980 | J Anim Sci1980，50，： | 2 |
| 3 | Serum levels of follicl-stimulating hormone during the bovine estrous cycle 显示文摘 | Akbar A M Reichert L E Jr Dunn T G Jr | 1974 | J Anim Sci1974，39，： | 1 |
| 4 | Transforming growth factor-betal modulates extracellular matrix production, proliferation, and apoptosis of endothelial progenitor cells in tissue-engineering scaffolds显示文摘 | Sales V L Engelmayr G C Jr Mettler B A | 2006 | Circulation2006，，： | 1 |
| 5 | The effects of transtympanic micropressure treatmentin people with unilateral Meniere's disease显示文摘 | GATES G A GREEN J D Jr TUCC1 D L | 2004 | ArchOtolaryngol Head Neck Surg2004，130，： | 1 |
| 6 | Apoptosis induced by the sonomechanical effects of low intensity pulsed ultrasound in a human leukemia cell line显示文摘 | Feril L B Jr Kondo T Cui Z G | | 0，，02： | 1 |
| 7 | Robust channel estimation for OFDM systems with rapid dispersive fading channels显示文摘 | Li Y(G) Cimini L J Jr Sollenberger N R | 1998 | IEEE Transactions on Communications1998，46，7： | 1 |
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| 10 | Chromatic uniqueness and equivalence of K4 -homeomorphs 显示文摘 | WHITEHEAD JR E G ZHAO L C | 1984 | J Graph Theory1984，8，： | 1 |
| 11 | Polymer nanocomposites: a new strategy for synthesizing solid electrolytes for rechargeable lithium batteries 显示文摘 | Krawiec W Scanlon L G Fellner Jr L P | 1995 | Journal Power Sources1995，54，2： | 1 |
| 12 | Assignment of skin notation for threshold limit values chemicals based on acute dermal toxicity 显示文摘 | KENNEDY Jr G L BROCK W J BANEBJEE A K | 1993 | Appl Occup Environ Hyg1993，8，1： | 1 |
| 13 | Crystal structure of bovine coronavirus spike pro- tein lectin domain 显示文摘 | Peng G Xu L Lin YL Chen L Pasquarella JR Holmes KV Li F | 2012 | J Biol Chem2012，287，41： | 1 |
| 14 | Dynamic model- ing of a dual clearance squeeze film damper:Part I test rigand dynamic model with one damper显示文摘 | Moraru L Keith T G Jr Dimofte F | 2003 | Tribology Trans- actions2003，46，2： | 1 |
| 15 | Puttheservice-pmfitchaintovo&显示文摘 | Heskett J L Jmes T O Loveman G W Sasserr W E Jr ScaLA | 1994 | Har-vard Business Review1994，72，2： | 1 |
| 16 | Tem- plate-synthesized nanoscopic gold particles: optical spectra and the effects of particle size and shape显示文摘 | Foss Jr C A Hornyak G L Stockert J A | 1994 | J Phys Chem1994，98，： | 1 |
| 17 | M-type phospholipase A2 receptor as target antigen in idiopathic mem- branous nephropathy 显示文摘 | BECK Jr L H BONEGIO R G B LAMBEAU G | 2009 | N Engl J Med2009，361，1： | 1 |
| 18 | Robust channel estimation for OFDM systems with rapid dispersive fading channels 显示文摘 | Li Y G Jr Cimini L J Sollenberger N R | 1998 | IEEE Transactions on Communications1998，46，7： | 1 |
| 19 | Dural sinus thrombosis and pseudotumor cerebri : unexpected complications of suboccipital craniotomy and translabyrinthine craniectomy 显示文摘 | Keiper G L Jr Sherman J D Tomsick T A | 1999 | J Neurosurg1999，91，2： | 1 |
| 20 | A new and rapid colorimetric determination of acetylcholinesterase activity显示文摘 | Ellman G L Courtney K D Andres Jr V | 1961 | Biochem Pharmacol1961，7，2： | 1 |
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