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| 1 | Effects of Flower Buds Extract of Tussilago farfara on Focal Cerebral Ischemia in Rats and Inflammatory Response in BV2 Microglia显示文摘Objective: To investigate the effects of the flower buds extract of Tussilago farfara Linné(Farfarae Flos; FF) on focal cerebral ischemia through regulation of inflammatory responses in activated microglia. Methods: Brain ischemia was induced in Sprague-Dawley rats by a transient middle cerebral artery occlusion(t MCAO) for 90 min and reperfusion for 24 h. Twenty rats were randomly divided into 4 groups(n=5 per group): normal, t MCAO-induced ischemic control, t MCAO plus FF extract 300 mg/kg-treated, and t MCAO plus MK-801 1 mg/kg-treated as reference drug. FF extract(300 mg/kg, p.o.) or MK-801(1 mg/kg, i.p.) was administered after reperfusion. Brain infarction was measured by 2,3,5,-triphenyltetrazolium chloride staining. Neuronal damage was observed by haematoxylin eosin, Nissl staining and immunohistochemistry using anti-neuronal nuclei(Neu N), anti-glial fibril ary acidic protein(GFAP), and antiCD11 b/c(OX42) antibodies in ischemic brain. The expressions of inducible nitric oxide synthase(i NOS), tumor necrosis factor(TNF-α), and hypoxia-inducible factor-1 a(HIF-1α) were determined by Western blot. BV2 microglial cel s were treated with FF extract or its main bioactive compound, tussilagone with or without lipopolysaccharide(LPS). Nitric oxide(NO) production was measured in culture medium by Griess assay. The expressions of i NOS, COX-2 and pro-inflammatory cytokines mRNA were analyzed by reverse transcription-polymerase chain reaction. The expression of i NOS, and COX-2 proteins, the phosphorylation of ERK1/2, JNK, and p38 MAPK and the nuclear expression of NF-κB p65 in BV2 cells were determined by Western blot. Results: FF extract significantly decreased brain infarctions in ischemic rats(P<0.01). The neuronal death and the microglia/astrocytes activation in ischemic brains were inhibited by FF extract. FF extract also suppressed i NOS, TNF-α, and HIF-1α expression in ischemic brains. FF extract(0.2 and 0.5 mg/m L, P<0.01) and tussilagone 20 and 50 μmol/L, P<0.01) significantly decreased LPS-induced NO production in BV2 microglia through downregulation of i NOS m RNA and protein expression. FF extract and tussilagone significantly inhibited LPS-induced expression of TNF-α, IL-1β, and IL-6 m RNA, and also suppressed the phosphorylation of ERK1/2, JNK and p38 MAPK and the nuclear expression of NF-κB in a dosedependent manner. Conclusion: FF extract has a neuroprotective effect in ischemic stroke by the decrease of brain infarction, and the inhibition of neuronal death and microglial activation-mediated inflammatory responses. | Ji Hye Hwang Vinoth Kumar R Seok Yong Kang Hyo Won Jung Yong-Ki Park | 2018 | Chinese Journal of Integrative Medicine2018，24，11： | 13 |
| 2 | Ultrasonic tissue characterization evaluates myocardial viability and ischemia in patients with coronary artery disease显示文摘 | LIN L C YEN R F HWANG J J | 2000 | Ultrasound Med Biol2000，26，5： | 2 |
| 3 | An image-guided mobile robotic welding system for SMAW repair processes 显示文摘 | Tung P C Wu M C Hwang Y R | 2004 | Internaotional Journal of Machine Tools & Manufacture2004，44，： | 1 |
| 4 | Trusted P2P transactions with fuzzy reputation aggregation 显示文摘 | SONG S S HWANG K ZHOU R F | 2005 | IEEE Internet Computing2005，9，6： | 1 |
| 5 | Wind-induced vibration of high-rise building with tuned mass damper including soil-structure interaction显示文摘 | Liu M Y Chiang W L Hwang J H Chu C R | 2008 | Journal of Wind Engineering and Industrial Aerodynamics2008，96，： | 1 |
| 6 | Oil composition variation and reservoir continuity : Unity Field Sudan 显示文摘 | Hwang R J | 1994 | Org Geoehem1994，21，2： | 1 |
| 7 | Capacitance enhancement of carbon fabric electrodes in electrochemical capacitors through electrodeposition with copper显示文摘 | Hwang S R Teng H | 2002 | J Electrochem Soc2002，149，5： | 1 |
| 8 | Mul-ticity trip patterns:Tourists to the United States显示文摘 | Hwang Yeong Hyeon Gretzel Ulrike Fesenmaier Daniel R | 2006 | Annals ofTourism Research2006，33，4： | 1 |
| 9 | Inhibition of platelet de rived growth factor receptor phosphorylation by STI571(Gleevec) reduces growth and metastasis of human pancreatic carcinoma in an orthotopic nude mouse model显示文摘 | HWANG R F YOKOI K BUCANA C D | 2003 | Clin Cancer Res2003，9，17： | 1 |
| 10 | A novel functional module detection algorithm for proteinprotein interaction networks显示文摘 | Hwang W Cho Y R Zhang A | 2006 | Algorithms for Molecular Biology2006，1，24： | 1 |
| 11 | PowerTrust:A Robust and ScalableReputation System for Trusted Peer-to-Peer Computing显示文摘 | R Zhou K Hwang | 2007 | IEEE Transactions on Parallel and DistributedSystems2007，18，4： | 1 |
| 12 | Development of collagenase-resistant collagen and its interaction with adult human dermal fibroblasts显示文摘 | Goo H C Hwang Y S Choi Y R | 2003 | Biomaterials2003，24，28： | 1 |
| 13 | On the virtual crack extension method for calculating the derivatives of energy release rates for 3D planar crack of arbitrary shape under mode-I loadinf显示文摘 | Hwang C G Wawrzynek P A Ingraffea A R | 2001 | Engineering Fracture Mechanics2001，68，： | 1 |
| 14 | High strain rate tensile properties of an(Al203 particles)-(A1 alloy 6061T6)metal matrix composite显示文摘 | PERNG C C HWANG J R DOONG J L | 1993 | Materials Science and Engineering A1993，171，12： | 1 |
| 15 | Power-trust: a robust and scalable reputation system for trusted peer-to-peer computing显示文摘 | ZHOU R F HWANG K | 2007 | IEEE Transactions on Parallel and Distributed Systems2007，18，4： | 1 |
| 16 | PowerTrust: a robust and scalable reputation system for trusted Peer-to-Peer computing显示文摘 | ZHOU R HWANG K | 2007 | IEEE Transactions on Parallel and Distributed Systems2007，18，5： | 1 |
| 17 | BriaexcavatinsM-P,four new briarane-related diterpenoids from cul-tured octocoral Briareum excavatum (Briareidae) 显示文摘 | Sung P J Lin M R Hwang T L | 2008 | Chem Pharm Bull2008，56，7： | 1 |
| 18 | Effect of synthesis conditions on electrochemical properties of LiNi1-yCoyO2 cathode for lithium rechargeable batteries 显示文摘 | Hwang B J Santhanam R Chen C H | 2003 | Journal of Power Sources2003，114，： | 1 |
| 19 | 查看详情显示文摘 | D K Hwang R R Dasari M Fenoll | | 0，，32： | 1 |
| 20 | Potential bene- fits of saturation cycle with two-phase refrigerant injection 显示文摘 | Lee H Hwang Y Radermacher R | 2013 | Applied Thermal Engineering2013，56，1： | 1 |
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