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| 1 | 靶向Jak/Stat通路作为Akt/β-联蛋白诱导的肝细胞癌SP/CD44+肿瘤细胞的治疗策略显示文摘【据《J Hepatol》2019年9月报道】题:靶向Jak/Stat通路作为Akt/β-联蛋白诱导的肝细胞癌SP/CD44+肿瘤细胞的治疗策略(作者Toh TB等)肝切除和肝移植以及辅助放化疗是肝细胞癌(HCC)治疗的主要手段。由于其频繁复发和肝内转移,HCC患者5年生存率仍然很差。目前仅索拉非尼和仑伐替尼被批准用于晚期、非切除的HCC的一线治疗,但其生存获益有限。因此需要确定新的治疗靶点以改进目前HCC治疗方式。 | 丁梦梦 高沿航 TOH TB LIM JJ HOOI L | 2020 | 临床肝胆病杂志2020，36，3： | 16 |
| 2 | ACSL4 is a predictive biomarker of sorafenib sensitivity in hepatocellular carcinoma显示文摘Sorafenib is the first-line treatment of advanced hepatocellular carcinoma(HCC).However,there is a lack of validated biomarkers to predict sorafenib sensitivity.In this study we investigated the role of ACSL4,a positive-activating enzyme of ferroptosis,in sorafenib-induced cell death and HCC patient outcome.We showed that ACSL4 protein expression was negatively associated with IC50 values of sorafenib in a panel of HCC cell lines(R=−0.952,P<0.001).Knockdown of ACSL4 expression by specific siRNA/sgRNA significantly attenuated sorafenib-induced lipid peroxidation and ferroptosis in Huh7 cells,and also rescued sorafenib-induced inhibition of xenograft tumor growth in vivo.We selected 29 HCC patients with surgery as primary treatment and sorafenib as postoperative adjunct therapy from a hospital-based cohort.A high proportion(66.7%)of HCC patients who had complete or partial responses to sorafenib treatment(according to the revised RECIST guideline)had higher ACSL4 expression in the pretreated HCC tissues,compared with those who had stable or progressed tumor growth(23.5%,P=0.029).Since ACSL4 expression was independent of sorafenib treatment,it could serve as a useful predictive biomarker.Taken together,this study demonstrates that ACSL4 is essential for sorafenib-induced ferroptosis and useful for predicting sorafenib sensitivity in HCC.This study may have important translational impacts in precise treatment of HCC. | Ji Feng Pei-zhi Lu Guang-zhi Zhu Shing Chung Hooi Yong Wu Xiao-wei Huang Hui-qi Dai Pan-hong Chen Zhong-jie Li Wen-jing Su Chuang-ye Han Xin-ping Ye Tao Peng Jing Zhou Guo-dong Lu | 2021 | Acta Pharmacologica Sinica2021，42，1： | 11 |
| 3 | Comparative studies of versatile extracellular proteolytic activities of lactic acid bacteria and their potential for extracellular amino acid productions as feed supplements显示文摘Background:Increasing understanding on the functions of amino acids (AA) has led to new commercial applications and expansion of the worldwide markets.However,the current technologies rely heavily on non-food grade microorganism and chemical synthesis for the production of AA.Several studies reported that lactic acid bacteria (LAB) have the capability of producing AA owing to their well-established proteolytic system and amino acid biosynthesis genes.Hence,the objectives of this study were to explore the extracellular proteolytic activity of LAB isolated from various Malaysian fermented foods and their potential to produce AA extracellularly as feed supplements.Results:All the studied LAB isolates were versatile extracellular protease producers,whereby extracellular protease activities were detected from acidic to alkaline pH (pH 5,pH 6.5,pH 8) using qualitative and quantitative proteolytic assays.The highest proteolytic activity at pH 5 (15.76 U/mg) and pH 8 (19.42 U/mg) was achieved by Lactobacillus plantarum RG14,while Lactobacillus plantarum RS5 exhibited the highest proteolytic activity of 17.22 U/mg at pH 6.5.As for the results of AA production conducted in de Man,Rogosa and Sharpe medium and analysed by high pressure liquid chromatography system,all LAB isolates were capable of producing an array of AA.Generally,Pediococcus sp.showed greater ability for AA production as compared to Lactobacillus sp.Moreover,the studied LAB were able to produce a few major feed supplement AA such as methionine,lysine,threonine and tryptophan.P.pentosaceus TL-3 recorded the highest methionine and threonine productivity of 3.72 mg/L/h and 5.58 mg/L/h respectively.However,L.plantarum I-UL4 demonstrated a lysine productivity of 1.24 mg/L/h,while P.acidilactici TP-6 achieved up to 1.73 mg/L/h of tryptophan productivity.Conclusion:All the 17 studied LAB isolates possessed versatile extracellular proteolytic system and have vast capability of producing various amino acids including a few major feed supplement AA such as methionine,lysine,threonine and tryptophan.Despite AA production was strain dependent,the studied LAB isolates possessed vast potential and can be exploited further as a bio-agent or an alternative amino acids and bioactive peptide producers. | Ye Heng Lim Hooi Ling Foo Teck Chwen Loh Rosfarizan Mohamad Norhani Abdullah | 2019 | Journal of Animal Science and Biotechnology2019，10，3： | 5 |
| 4 | 多肽和蛋白质的反相高效液相色谱研究——色谱行为的多样性显示文摘以C8 柱为固定相 ,研究了微管结合蛋白2中两段重要肽段(肽1 ,肽2)和猪胰岛素在反相色谱上的保留行为。当以甲醇溶液为流动相时 ,猪胰岛素保留因子的对数值(lnK′)随流动相有机溶剂体积分数(φ)的变化呈现很好的线性关系。当以乙腈为流动相测定肽1的lnK′随 φ变化关系时 ,所得结果显示lnK′与 φ呈现良好的二次曲线关系。以乙腈为流动相测定肽1和肽2的Van'tHoff曲线(lnK′与1/T的关系图)时 ,发现两种多肽的Van'tHoff曲线分别呈二次和三次方曲线。所有这些结果说明多肽和蛋白质在反相色谱中保留行为的复杂性。 | 郭敏亮 Milton T W HEARN Hooi Hong KEAH | 2000 | 分析测试学报2000，19，4： | 4 |
| 5 | Lentiviral vector-mediated down-regulation of IL-17A receptor in hepatic stellate cells results in decreased secretion of IL-6显示文摘AIM: To investigate the mechanism of interleukin (IL)-6 secretion through blocking the IL-17A/IL-17A recepto (IL-17RA) signaling pathway with a short hairpin RNA (shRNA) in hepatic stellate cells (HSCs) in vitro . METHODS: HSCs were derived from the livers of adul male Sprague-Dawley rats. IL-6 expression was evalu ated using real-time quantitative polymerase chain reaction and enzyme linked immunosorbent assay. The phosphorylation activity of p38 mitogen activated pro tein kinases (MAPK) and extracellular regulated pro tein kinases (ERK) 1/2 upon induction by IL-17A and suppression by IL-17RA shRNA were examined using Western blotting.RESULTS: IL-6 expression induced by IL-17A was significantly increased compared to control in HSCs (P < 0.01 in a dose-dependent manner). Suppression of IL17RA using lentiviral-mediated shRNA inhibited IL-6 expression induced by IL-17A compared to group with only IL-17A treatment (1.44 ± 0.17 vs 4.07 ± 0.43, P < 0.01). IL-17A induced rapid phosphorylation of p38 MAPK and ERK1/2 after 5 min exposure, and showed the strongest levels of phosphorylation of p38 MAPK and ERK1/2 at 15 min in IL-17A-treated HSCs. IL-6 mRNA expression induced by IL-17A (100 ng/mL) for 3 h exposure was inhibited by preincubation with specific inhibitors of p38 MAPK (SB-203580) and ERK1/2 (PD-98059) compared to groups without inhibitors preincubation (1.67 ± 0.24, 2.01 ± 0.10 vs 4.08 ± 0.59, P < 0.01). Moreover, lentiviral-mediated IL-17RA shRNA 1 inhibited IL-17A-induced IL-6 mRNA expression compared to random shRNA in HSCs (1.44 ± 0.17 vs 3.98 ± 0.68, P < 0.01). Lentiviral-mediated IL17RA shRNA 1 inhibited phosphorylation of p38 MAPK and ERK1/2 induced by 15 min IL-17A (100 ng/mL) exposure. CONCLUSION: Down-regulation of the IL-17RA receptor by shRNA decreased IL-6 expression induced by IL-17A via p38 MAPK and ERK1/2 phosphorylation in HSCs. Suppression of IL-17RA expression may be a strategy to reduce the inflammatory response induced by IL-17A in the liver. | Sheng-Chu Zhang Yi-Hu Zheng Pan-Pan Yu Tan Hooi Min Fu-Xiang Yu Chao Ye Yuan-Kang Xie Qi-Yu Zhang | 2012 | World Journal of Gastroenterology2012，18，28： | 3 |
| 6 | Development of an adaptive thermal comfort equation for naturally ventilated buildings in hot-humid climates using ASHRAE RP-~4 dataha~显示文摘 | Doris Hooi Chyee Toe Tetsu Kubota | 2013 | Frontiers of Architectural Research2013，2，3： | 3 |
| 7 | Eosinophilic cholangitis treatment with budesonide显示文摘Eosinophilic cholangitis is a rare cause of deranged obstructive liver function tests. It has been described as a great mimicker for malignant biliary strictures and bile duct obstruction. There are only case reports available on treatment experience for eosinophilic cholangitis. A large proportion of patients present with biliary strictures for which they have undergone surgery or endoscopic treatment and a small proportion was given systemic corticosteroid. We share our treatment experience using budesonide which has fewer systemic side effects to prednisolone and avoids invasive management. | Marianne Anastasia De Roza Chee Hooi Lim | 2017 | World Journal of Hepatology2017，9，36： | 2 |
| 8 | Multicenter randomised controlled trial comparing the high definition white light endoscopy and the bright narrow band imaging for colon polyps显示文摘AIM To compare high definition white light endoscopy and bright narrow band imaging for colon polyps' detection rates. METHODS Patients were randomised to high definition white light endoscopy(HD-WLE) or the bright narrow band imaging(b NBI) during withdrawal of the colonoscope. Polyps identified in either mode were characterised using b NBI with dual focus(b NBI-DF) according to the Sano's classification. The primary outcome was to compare adenoma detection rates(ADRs) between the two arms. The secondary outcome was to assess the negative predictive value(NPV) in differentiating adenomas from hyperplastic polyps for diminutive rectosigmoid lesions.RESULTS A total of 1006 patients were randomised to HD-WLE(n = 511) or b NBI(n = 495). The mean of adenoma per patient was 1.62 and 1.84, respectively. The ADRs in b NBI and HD-WLE group were 37.4% and 39.3%, respectively. When adjusted for withdrawal time(OR = 1.19, 95%CI: 1.15-1.24, P < 0.001), the use of b NBI was associated with a reduced ADR(OR = 0.69, 95%CI: 0.52-0.92). Nine hundred and thirty three polyps(86%) in both arms were predicted with high confidence. The sensitivity(Sn), specificity(Sp), positive predictive value and NPV in differentiating adenomatous from non-adenomatous polyps of all sizes were 95.9%, 87.2%, 94.0% and 91.1% respectively. The NPV in differentiating an adenoma from hyperplastic polyp using b NBI-DF for diminutive rectal polyps was 91.0%.CONCLUSION ADRs did not differ between b NBI and HD-WLE, however HD-WLE had higher ADR after adjustment of withdrawal time. b NBI surpassed the PIVI threshold for diminutive polyps. | Rajvinder Singh Kuan Loong Cheong Leonardo Zorron Cheng Tao Pu Dileep Mangira Doreen Siew Ching Koay Carmen Kee Siew Chien Ng Rungsun Rerknimitr Satimai Aniwan Tiing-Leong Ang Khean-Lee Goh Shiaw Hooi Ho James Yun-Wong Lau | 2017 | World Journal of Gastrointestinal Endoscopy2017，9，6： | 2 |
| 9 | Asymptomatic peripheralarterial occlusive disease predicted cardiovascular morbidity and motality in a 7--year--follow--up study显示文摘 | Hooi JD Kester AD Stoffcrs HE | 2004 | J Clin Epidemiol2004，57，3： | 1 |
| 10 | The prognosis of non-critical limb ischaemia: a systematic review of populationbased evidence显示文摘 | Hooi JD Stoffers HE Knottnerus JA | 1999 | Br J Gen Pract1999，49，438： | 1 |
| 11 | Asymptomatic peripheral arterial occlusive disease predicted cardiovascularmordibity and mortality in a 7-year follow-up study显示文摘 | Hooi JD Kester AD Stoffers HE | 2004 | J Clin Epidemiol2004，57，3： | 1 |
| 12 | Randomized controlled trial of pulse intravenous cyclophosphamide versus mycophenolate mofetil in the induction therapy of proliferative lupus nephritis显示文摘 | Ong LM Hooi LS Lim TO | 2005 | Nephrology(Carlton)2005，10，5： | 1 |
| 13 | Asymptomatic peripheral arterial occlusive disease predicted cardiovascular morbidity and motality in a 7-year-follow-up study显示文摘 | Hooi J D Kester AD Stoffers HE | 2004 | J Clin Epidemiol2004，57，3： | 1 |
| 14 | Asymptomatic peripheral arterial occlusive disease predicted cardiovascular mordibity and mortality in a 7-year follow-up study显示文摘 | Hooi JD Kester AD Stoffers HE | 2004 | J Clin Epidemiol2004，57，3： | 1 |
| 15 | Asymptomatic peripheral arterial occlusive disease predicted cardiovascular morbidity and mortality in a 7-year followup study显示文摘 | Hooi JD Kester AD Stoffers HE | 2004 | J Clin Epidemiol2004，57，3： | 1 |
| 16 | Asymptomatic peripheral occlusive disease predicted cardiovascular morbidity and mortality in a 7 - year fol- low up study 显示文摘 | Hooi JD Kester AD Stoffers HE | 2004 | J Clin Epidermal2004，57，3： | 1 |
| 17 | Randomized con- trolled trial of pulse intravenous cyclophosphamide versus mycophenolate mofetil in the induction therapy of prolifer- ative lupus nephritis显示文摘 | Ong LM Hooi LS Lim TO | | Nephrology (Carlton)0，10，5： | 1 |
| 18 | Occurrence,structure,biochemical properties and technological characteristics of lactoferrin 显示文摘 | Stei jins JM Van Hooi Jdonk AC | 2000 | Br J Nutr2000，84，1： | 1 |
| 19 | Randomized controlled trial of pulse intravenous cyclophosphamide versus mycophenolate mofetil in the induction therapy of proliferate lupus nephritis 显示文摘 | Ong LM Hooi LS Lim TO | 2005 | Nephrology2005，10，5： | 1 |
| 20 | Does financial develop- ment increase energy consumption?The role of industrialization and urbanization in Tunisia显示文摘 | Muhammad Shahbaz Hooi Hooi Lean | 2012 | Energy Policy2012，，40： | 1 |
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