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| 1 | Clinical and epidemiological characteristics of norovirus gastroenteritis among hospitalized children in Lebanon显示文摘AIM To assess the burden of norovirus(No V) and to determine the diversity of circulating strains among hospitalized children in Lebanon. METHODS Stool samples were collected from children presenting with acute gastroenteritis to six major hospitals in Lebanon. A total of 739 eligible stool samples, testing negative for diarrhea caused by rotavirus as a possible viral pathogen, were collected between January 2011 and June 2013. A standardized questionnaire including demographic, epidemiological and clinical observations was used at the time of hospitalization of children presenting with diarrhea. Viral RNA was extracted from stool samples followed by reverse transcription polymerase chain reaction and nucleotide sequencing of a fragment of the viral protein 1 capsid gene. Multiple sequence alignments were carried out and phylogenetic trees were constructed using the MEGA 6 software.RESULTS Overall, 11.2% of stool samples collected from children aged < 5 years tested positive for No V genogroups Ⅰ(GⅠ) and Ⅱ(GⅡ). GⅡ accounted for 10.6% of the gastroenteritis cases with only five samples being positive for GⅠ(0.7%). The majority of hospitalized children showed symptoms of diarrhea, dehydration, vomiting and fever. Upon sequencing of positive samples and based on their clustering in the phylogenetic tree, 4/5 of GⅠ gastroenteritis cases were designated GⅠ.3 and one case as GⅠ.4. GⅡ.4 was predominantly detected in stool of our study participants(68%). We report a JB-15/KOR/2008 GⅡ.4 Apeldoorn 2008-like variant strain circulating in 2011; this strain was replaced between 2012 and 2013 by a variant sharing homology with the Sydney/NSW0514/2012/AUS GⅡ.4 Sydney 2012 and Sydney 2012/FRA GⅡ.4 strains. We also report the co-circulation of non-GⅡ.4 genotypes among hospitalized children. Our data show that No V gastroenteritis can occur throughout the year with the highest number of cases detected during the hot months.CONCLUSION The majority of No V-associated viral gastroenteritis cases among our participants are attributable to GⅡ.4, which is compatible with results reported worldwide. | Nada M Melhem Hassan Zaraket Khalil Kreidieh Zeinab Ali Moza Hammadi Soha Ghanem Farah Hajar Amjad Haidar Adlette Inati Mariam Rajab Hassan Fakhouri Bassam Ghanem Ghassan Baasiri Ghassan Dbaibo | 2016 | World Journal of Gastroenterology2016，22，48： | 3 |
| 2 | Benefits of Soy Isoflavone Therapeutic Regimen on Menopausal Symptoms显示文摘 | Han K K Soares J M Haidar M A | 2002 | Obstet Gynecol2002，99，3： | 1 |
| 3 | Soil solarization and chicken manure for the control of Orobanche crenata and other weeds in Lebanon显示文摘 | Haidar M A Sidahmed M M | 2000 | Crop Protection2000，19，3： | 1 |
| 4 | Benefits of Soy lsoflavone Therapeutic Regimen on Menopausal Symptoms 显示文摘 | Han K K Soares J M Haidar M A | 2002 | Obstet Gynecol2002，99，3： | 1 |
| 5 | Benefits of soy isoflavone, therapeutic regimen on menopau -sal symptoms显示文摘 | HANK K SOARES J M HAIDAR M A | 2002 | Am College Obstet Gynccol2002，99，3： | 1 |
| 6 | Glucose-responsive insulin and glucagon delivery (dual-hormone artificial pancreas) in adults with type 1 diabetes: a randomized crossover controlled trial 显示文摘 | Haidar A Legault L Dallaire M | 2013 | CMAJ2013，185，4： | 1 |
| 7 | Effects of structure on pyrolysis gases from amino acids 显示文摘 | Haidar N F Patterson J M Moors M | 1981 | Journal of Agricultural and Food Chemistry1981，29，1： | 1 |
| 8 | Utilization of picohydro generation in domestic and commercial loads显示文摘 | Ahmed M A Haidar Mohd F M Senan Abdulhakim Noman | 2012 | Renewable and Sustainable Energy Reviews2012，1，16： | 1 |
| 9 | Pyrolysis of phenylalanine, 3,6-dibenzyl-2,5-piperazinedione, and phenethylamine 显示文摘 | Patterson J M Haidar N F Papadopoulos E P | 1973 | The Journal of Organic Chemistry1973，38，4： | 1 |
| 10 | Molecular interactions be- tween apoE and ABCA1 : impact on apoE lipidation 显示文摘 | Krimbou L Denis M Haidar B | 2004 | J Lipid Res2004，45，： | 1 |
| 11 | Techniques for quantitative analyses of calcareous marine phytoplankton显示文摘 | Bollmann J Corts M Y Haidar A T | 2002 | Marine Micropaleontolo gy2002，44，34： | 1 |
| 12 | Apolipoprotein A- Iactivates cellular cAMP signaling through the ABCAI transporter显示文摘 | Haidar B Denis M Marcil M | 2004 | J Biol Chem2004，279，： | 1 |
| 13 | Glucose-responsive insulin and glucagon delivery(dual-hormone artiifcial pancreas)in adults with type 1 diabetes:a randomized crossover controlled trial显示文摘 | Haidar A Legault L Dallaire M | | 0，，： | 1 |
| 14 | Effects of structure on pyrolysis gases from amino acids 显示文摘 | Haidar N F Patterson J M Moors M | 1981 | Journal of Agricultural and Food Chemistry1981，29，1： | 1 |
| 15 | Benefits of soy isoflavone therapeutic regimen on menopausal symptoms显示文摘 | Han K K Soares J M Haidar M A Rodrigues de Lima G Baracat E C | 2002 | Obstet Gynecol2002，99，3： | 1 |
| 16 | An Entropy Approach for Abnormal Activities Detection in Video Streams 显示文摘 | Haidar Sharif M Djeraba C | 2012 | Pattern Recognition2012，45，7： | 1 |
| 17 | Apolipoprotein A-I activates cellular cAMP signaling through the ABCA1 transporter显示文摘 | Haidar B Denis M Marcil M | 2004 | J Biol Chem2004，279，11： | 1 |
| 18 | Transientstability evaluation of electrical power system usinggeneralized regression neural networks 显示文摘 | Haidar A M A Mustafa M W Ibrahim F AF | 2011 | Applied SoftComputing2011，22，： | 1 |
| 19 | Benefits of soy isoflavone the rapeutic regimen on menopausal symptoms显示文摘 | Han K K Soares J M Haidar M A | 2002 | Obstet Gynecol2002，99，3： | 1 |
| 20 | ApolipoproteinA-I activates cellular cAMP signaling through the ABCA1 transporter显示文摘 | Haidar B Denis M Marcil M | 2004 | J Biol Chem2004，279，11： | 1 |
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