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| 1 | Effect of Helicobacter pylori cdrA on interleukin-8 secretions and nuclear factor kappa B activation显示文摘AIM:To investigate genetic diversity of Helicobacter pylori (H.pylori) cell division-related gene A (cdrA) and its effect on the host response.METHODS:Inactivation of H.pylori cdrA,which is involved in cell division and morphological elongation,has a role in chronic persistent infections.Genetic property of H.pylori cdrA was evaluated using polymerase chain reaction and sequencing in 128 (77 American and 51 Japanese) clinical isolates obtained from 48 and 51 patients,respectively.Enzyme-linked immunosorbent assay was performed to measure interleukin-8 (IL-8) secretion with gastric biopsy specimens obtained from American patients colonized with cdrA-positive or-negative strains and AGS cells cocultured with wild-type HPK5 (cdrA-positive) or its derivative HPKT510 (cdrA-disruptant).Furthermore,the cytotoxin-associated gene A (cagA) status (translocation and phosphorylation) and kinetics of transcription factors [nuclear factor-kappa B (NF-κB) and inhibition kappa B] were investigated in AGS cells co-cultured with HPK5,HPKT510 and its derivative HPK5CA (cagA disruptant) by western blotting analysis with immunoprecipitation.RESULTS:Genetic diversity of the H.pylori cdrA gene demonstrated that the cdrA status segregated into two categories including four allele types,cdrA-positive (allele types;Ⅰand Ⅱ) and cdrA-negative (allele types;Ⅲ and Ⅳ) categories,respectively.Almost all Japanese isolates were cdrA-positive (Ⅰ:7.8% and Ⅱ:90.2%),whereas 16.9% of American isolates were cdrA-positive (Ⅱ) and 83.1% were cdrA-negative (Ⅲ:37.7% and Ⅳ:45.5%),indicating extended diversity of cdrA in individual American isolates.Comparison of each isolate from different regions (antrum and corpus) in the stomach of 29 Americans revealed that cdrA status was identical in both isolates from different regions in 17 cases.However,12 cases had a different cdrA allele and 6 of them exhibited a different cdrA category between two regions in the stomach.Furthermore,in 5 of the 6 cases possessing a different cdrA category,cdrA-negative isolate existed in the corpus,suggesting that cdrA-negative strain is more adaptable to colonization in the corpus.IL-8 secretions from AGS revealed that IL-8 levels induced by a cdrA-disrupted HPKT510 was significantly lower (P < 0.01) compared to wildtype HPK5:corresponding to 50%-60% of those of wild-type HPK5.These data coincided with in vivo data that an average value of IL-8 in biopsy specimens from cdrA-positive and cdrA-negative groups was 215.6 and 135.9 pg/mL,respectively.Western blotting analysis documented that HPKT510 had no effect on CagA translocation and phosphorylation,however,nuclear accumulation of NF-κB was lower by HPKT510 compared to HPK5.CONCLUSION:Colonization by a cdrA-negative or cdrA-dysfunctional strain resulted in decreased IL-8 production and repression of NF-κB,and hence,attenuate the host immunity leading to persistent infection. | Hiroaki Takeuchi Ya-Nan Zhang Dawn A Israel Richard M Peek Jr Mikio Kamioka Hideo Yanai Norihito Morimoto Tetsuro Sugiura | 2012 | World Journal of Gastroenterology2012，18，5： | 3 |
| 2 | Differential actions of cardioprotective agents on the mitochondrial death pathway 显示文摘 | Akao M O′Rourke B Hideo Kusuoka H | 2003 | Circ Res2003，92，2： | 1 |
| 3 | Impaired glucose tolerance is a risk factor of cardiovaseular disease, but not impaired fasting glueose:the Funagata diabetes study显示文摘 | Makoto T Hideyuki E Hideo M | 1999 | Diabetes Care1999，22，： | 1 |
| 4 | Incresed vascular endothelial cell markers in patients with disseminated intravascular coagulation显示文摘 | Hideo W Kouzou M Yoshihiro W | 1993 | Am J Hematol1993，44，2： | 1 |
| 5 | Role of uridine phosphorylase for antitumor activity of 5 '-deoxy-5-fluorouridine 显示文摘 | Hideo I Masanori M Kenji T | 1980 | Gann1980，71，2： | 1 |
| 6 | Nanocomposites-a new material design concept显示文摘 | Seong M C Hideo A | 2005 | Science and Technology of Advanced Materials2005，6，1： | 1 |
| 7 | Genetic analysis and molecular characterisation of laboratory and field mutants of Botryotinia fuckeliana ( Botrytis cinerea ) resistant to QoI fungicides显示文摘 | Rita M De Miccolis Angelini Caterina Rotolo Mario Masiello Stefania Pollastro Hideo Ishii Francesco Faretra | 2012 | Pest Manag Sci2012，，9： | 1 |
| 8 | Clinical outcomes of short Hook-wire and suture marking system in thoracoscopic resection for pulmonary nodules显示文摘 | SKentaroh M Shinichi T Hideo G | | 0，，02： | 1 |
| 9 | Detection of recombinant DNA segments introduced to genetically modified maize (Zea mays)显示文摘 | Takeshi M Hideo K Ken T | | 0，，07： | 1 |
| 10 | Canine model of obstructive sleep apnea:model description and preliminary application显示文摘 | Kimoff John R Hideo M | 1994 | J Appl Physiol1994，76，： | 1 |
| 11 | Expression of genes responsible for biomineralization of Pinctadafucata during development显示文摘 | YOKO M TAKUMA N HIDEO A | | 0，，03： | 1 |
| 12 | Advanced copper electroplating for application of electronics显示文摘 | SHUHEI M HIDEO H | 2003 | Surface and Coatings Technology2003，，： | 1 |
| 13 | Heat Transfer and Pressure Drop of a Supercritical Pressure Fluid Flowing in a Tube of Small Diameter显示文摘 | Tohru Y Hideo M Suguru Y | | 0，，04： | 1 |
| 14 | Inhibition of intimal hyperplasia after ballon injury by antibodies to intercellular adhesion moleclule-1 and lymphocyte function-associated antigen-1 显示文摘 | Hideo Y Tsutomu I Hidehiro M | 1997 | Circulation1997，95，： | 1 |
| 15 | Mechanisms of toughening and strengthening in ceramic-based nanocomposites显示文摘 | Hideo A Seong M C Eisuke Y | 2002 | Mechanics of Materials2002，34，： | 1 |
| 16 | Experimental study on electrostatic atomization of highly viscous liquids 显示文摘 | Hideo W Tatsushi M Hideo Y | 2003 | Journal of Electrostatics2003，，57： | 1 |
| 17 | Flocculation of diatomite by a soy protein-based bioflocculant 显示文摘 | Hideshi S Hideo M Yasuhiro S | 2010 | Biochemical Engineering Journal2010，51，2： | 1 |
| 18 | Alumina-ceria-zirconia composite oxide for three-way catalyst 显示文摘 | Tadashi S Akira M Akihiko S Hideo S Masahiro S Takaaki K Juji Si Toshihiro T | 2008 | R&D Review of Toyota CRDL2008，37，4： | 1 |
| 19 | Detoxification of groundnut meals with ozone显示文摘 | Hideo M H Growther P C Francis G J | 1988 | J Food Sci1988，53，2： | 1 |
| 20 | Canine model of obstructive sleep apnea: model description and preliminary application 显示文摘 | Kimoff John R Hideo M | 1994 | J Appl Physiol1994，76，： | 1 |
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