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| 1 | Hypoxia and cytokines regulate carbonic anhydrase 9 expression in hepatocellular carcinoma cells in vitro显示文摘AIM: To study the expression of carbonic anhydrase(CA) 9 in human hepatocellular carcinoma(HCC) cells.METHODS: We studied CA9 protein, CA9 m RNA and hypoxia-inducible factor-1 alpha(HIF-1α) protein levels in Hep3 B cells exposed in different parallel approaches. In one of these approaches, HCC cells were exposed to extreme in vitro hypoxia(24 h 0.1% O2) without or with interleukin(IL)-1, IL-6, tumor necrosis factoralpha(TNF-α) and transforming growth factor-beta(TGF-β) stimulation for the same hypoxic exposure time or exposed to normoxic oxygenation conditions without or with cytokine stimulation.RESULTS: The tumour cell line analysed showed a strong hypoxic CA9 m RNA expression pattern in response to prolonged severe hypoxia with cell-line specific patterns and a marked induction of CA9 protein in response to severe hypoxia. These results were paralleled by the results for HIF-1α protein under identical oxygenation conditions with a similar expression tendency to that displayed during the CA9 protein expression experimental series. Continuous stimulation with the cytokines, IL-1, IL-6, TNF-α and TGF-β, under normoxic conditions significantly increased the carbonic anhydrase 9 expression level at both the protein and m RNA level, almost doubling the CA9 m RNA and CA9 and HIF-1α protein expression levels found under hypoxia. The findings from these experiments indicated that hypoxia is a positive regulator of CA9 expression in HCC, and the four signal transduction pathways, IL-1, IL-6, TNF-α and TGF-β, positively influence CA9 expression under both normoxic and hypoxic conditions.CONCLUSION: These findings may potentially be considered in the design of anti- cancer therapeutic approaches involving hypoxia-induced or cytokine stimulatory effects on expression. In addition, they provideevidence of the stimulatory role of the examined cytokine families resulting in an increase in CA9 expression under different oxygenation conditions in human cancer, especially HCC, and on the role of the CA9 gene as a positive disease regulator in human cancer. | Feray Kockar Hatice Yildrim Rahsan Ilikci Sagkan Carsten Hagemann Yasemin Soysal Jelena Anacker Ahmed Ayad Hamza Dirk Vordermark Michael Flentje Harun M Said | 2012 | World Journal of Clinical Oncology2012，3，6： | 2 |
| 2 | Enhanced invasive- ness of breast cancer cell lines upon co-cultivation with macro- phages is due to TNF-alpha dependent up-regulation of matrix metalloproteases 显示文摘 | Hagemann T Robinson SC Schulz M | 2004 | Carcinogenesis2004，，： | 1 |
| 3 | Distribution of glutamate receptor subunits in experimentally induced cortical malformations显示文摘 | HAGEMANN G KLUSKA M M REDECKER C | 2003 | Neuroscience2003，117，4： | 1 |
| 4 | Electrophysiological transcortical diaschisis after cortical photothrombosis in rat brain显示文摘 | Buchkremer-Ratzmann I August M Hagemann G | 1996 | Stroke1996，27，： | 1 |
| 5 | Risk factors for inadequate emergence after anesthesia:emergence delirium and hypoactive emergence显示文摘 | Radtke FM Franck M Hagemann L | | 0，，06： | 1 |
| 6 | Xenon purity analysis for exo-200 via mass spectrometry 显示文摘 | Dobi A Hall C Slutsky S Yen Y R Aharmin B Auger M Barbeau P S Benitez-Medina C Breidenbach M Cleveland B Conley R Cook J Cook S Counts I Craddock W Daniels T Davis C G Davis J Devoe R Dixit M Dolinski M J Donato K Fairbank Jr W Farine J Fierlinger P Franco D Giroux G Gornea R Graham K Gratta G Green C Hagemann C Hall K Hallman D Hargrove C Herrin S Hughes M Hodgson J Juget F Karelin A Kaufman L J Kuchenkov A Kumar K Leonard D S Lutter G Mackay D Maclellan R Marino M Mong B Montero Diez M Morgan P MUller A R Neilson R Odian A Oullivan K Piepke A Pocar A Prescott C Y Pushkin K Rivas A Rollin E Rowson P C Sabourov A Sinclair D Skarpaas K Stekhanov V Strickland V Swift M Twelker K Vuilleumier J L Vuilleumier J M Weber M Wichoski U Wodin J Wright J D Yang L | 2012 | Nuclear Instruments and Methods in Physics Research Section A: Accelerators Spectrometers Detectors and Associated Equipment2012，675，： | 1 |
| 7 | Stabilization of model membranes during drying by compatible solutes involved in the stress tolerance of plants and microorganisms显示文摘 | Hincha D K Hagemann M | 2004 | Biochem J2004，383，： | 1 |
| 8 | Risk factors for inadequate emergence after anesthesia:emergemce delirium and hypoaelive emergence显示文摘 | Radtke F M Franck M Hagemann L | | 0，，06： | 1 |
| 9 | The cytological basis of plastid inheritance in angiosperms显示文摘 | Hagemann R Schroder M B | 1989 | Protoplasma1989，15，： | 1 |
| 10 | Orogenic gold deposits: A proposed classification in the context of their crustal distribution and relationship to other gold deposit types显示文摘 | D.I Groves R.J Goldfarb M Gebre-Mariam S.G Hagemann F Robert | 1998 | Ore Geology Reviews1998，，1： | 1 |
| 11 | Risk factors for inadequate emergence after anesthesia: emergence delirium and hypoactive emergence显示文摘 | RADTKE F M FRANCK M HAGEMANN L | 2010 | Minerva Anestesiol2010，76，6： | 1 |
| 12 | Prevention and treatment of oral mucositis in children with cancer 显示文摘 | Miller M M Donald D V Hagemann T M | 2012 | J Pediatr Pharmacol Ther2012，17，4： | 1 |
| 13 | Redirected anti- tumor activity of primary human lymphocytes transduced with a fully human anti-mesothelin chimeric receptor 显示文摘 | Lanitis E Poussin M Hagemann IS | 2012 | Mol Ther2012，20，3： | 1 |
| 14 | Activation and pathway of glucosylglycerolsynthesis in the cyanobacterium Synechocystis PCC6803 显示文摘 | Hagemann M Erdmann N | 1994 | Microbiology1994，140，： | 1 |
| 15 | A classification scheme for epigenetic Archean lode-gold deposits 显示文摘 | Gebre-Mariam M Hagemann S G Groves D I | 1995 | Miner Deposita1995，30，： | 1 |
| 16 | Evaluation of methods used in testing wheat susceptibility to pre-harvest sprouting显示文摘 | Hagemann M C Ciha A J | 1984 | Crop Science1984，24，： | 1 |
| 17 | Evaluation of methods used in resting winter wheat susceptibility to perharvest spurouting显示文摘 | Hagemann M G A J ciha | 1984 | Crop Science1984，24，2： | 1 |
| 18 | Compatible solute biosynthesis in cyanobacteria 显示文摘 | Klahn S Hagemann M | 2011 | Environmental Microbiology2011，13，3： | 1 |
| 19 | Enhanced invasiveness of breast cancer cell lines upon co-cultivation with macrophages is due to TNF-alpha dependent up-regulation of matrix metalloproteases 显示文摘 | Hagemann T Robinson SC Sehulz M | 2004 | Carcinogenesis2004，25，8： | 1 |
| 20 | Evaluation of methods used in testing wheat susceptibility to preharvest sprouting显示文摘 | Hagemann M G Ciha A J | 1984 | Crop Sci1984，24，2： | 1 |
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