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| 1 | Destabilization of acrosome and elastase influence mediate the release of secretory phospholipase A2 from human spermatozoa显示文摘 | Jacqueline Leβig Uta Reibetanz Jiirgen Arnhold Hans-Jtirgen Glander | 2008 | Asian Journal of Andrology2008，10，6： | 2 |
| 2 | Immunomagnetic removal of cryo-damaged human spermatozoa显示文摘Aim: To estimate the dissipation of mitochondrial transmembrane potential (mTMP, △ψm) and activation of sperm caspases (aCP) as signs of apoptosis in human spermatozoa during cryopreservation and to evaluate the efficiency of immunomagnetic cell separation (MACS) of these spermatozoa via annexin V-binding. Methods: The mTMP and aCP in fresh and cryopreserved spermatozoa were detected by fluorescence microscopy and by Western blots. The sperm suspensions were divided into two sperm fractions (with intact and deteriorated membranes) by magnetic cell separation (MiniMACS, Miltenyi Biotec, Bergisch Gladbach, Germany) in dependence on their binding to superparamagnetic annexin V-microbeads (AN-MB). Results: The cryopreservation decreased the portion of spermatozoa with intact mTMP from 80.1% ± 7.2 % to 53.5 % ± 13.1% and increased the spermatozoa with activated pancaspases (aCP) from 21.8 % ± 2.6 % to 47.7 % ± 5.8 % (n = 10; mean ± SEM; P < 0.01). The activation of caspases 1, 3, 8, and 9 in the cryopreserved spermatozoa was confirmed by Western blots (n = 22). MACS reduced significantly the percentage of cryopreserved spermatozoa with dissipated mTMP to 8.1 ± 3.9 (P < 0.01) and also those with aCP to 9.3 % ± 2.2 %. Western blot analyses confirmed the increase of the activated caspase3, 9, and 8 in the AN-MB-positive fraction (P < 0.05) compared with the AN-MB-negative fraction. The MACS separation effect was confirmed by anti-annexin V-antibodies. There was no significant influence of the separation column and the magnetic field on the sperm functions. Conclusion: The cryopreservation impaired the mTMP and enhanced the activation status of caspases in human spermatozoa. The immunomagnetic sperm separation via binding of AN-MB could deplete low quality spermatozoa from cryopreserved semen samples. | Uwe Paasch Sonja Grunewald Katja Wuendrich Tbrsten Jope Hans-Jurgen Glander | 2005 | Asian Journal of Andrology2005，7，1： | 2 |
| 3 | Donor-specific HLA antibodies in a cohort comparing everolimus with cyclosporine after kidney transplantation 显示文摘 | Liefeldt L Brakemeier S Glander P | 2012 | Am J Transplant2012，12，5： | 1 |
| 4 | Role of caspases in male infertility显示文摘 | Said TM Paasch U Glander HJ Agarwal A | 2004 | Hum Reprod Update2004，10，1： | 1 |
| 5 | The impact of plant secondary compounds on primate feeding behavior 显示文摘 | Glander K E | 1981 | Yrbk Phys Anthro1981，25，： | 1 |
| 6 | Insulin- like growth factor-I and alpha 2-macroglobulin in seminal plasma correlate with semen quality 显示文摘 | Glander HJ Kratzsch J Weisbrich C | 1996 | Hum Reprod1996，11，11： | 1 |
| 7 | A coprologicalsurvey of parasites of wild mantled howling monkeys,Alouatta palliatapalliate显示文摘 | STUART M D GREENSPAN L L GLANDER K E | 1990 | Journal of Wildlife Diseases1990，26，： | 1 |
| 8 | Pharmacodynamic monitoring ofmycophenolate mofetil 显示文摘 | BUDDE K GLANDER P BAUER S | 2000 | Clin Chem Lab Med2000，38，11： | 1 |
| 9 | Agedependent inhibin B concentration in relation to FSH and semen sample qualities: a study in 2448 men显示文摘 | GRUNEWALD S GLANDER HJ PAASCH U | 2013 | Reproduction2013，145，3： | 1 |
| 10 | Abstract representations for interac- tive visualization of virtual 3D city models显示文摘 | Glander T Drllner J | 2009 | Comput- ers Environment and Urban Systems2009，33，5： | 1 |
| 11 | Binding of annexin V to plasma membranes of human spermatozoa: a rapid assay for detection of membrane changes after cryostorage显示文摘 | Glander HJ | 1999 | Mol Hum Reprod1999，5，2： | 1 |
| 12 | Role of caspases in male infertility update显示文摘 | Said T Paasch U Glander H | 2004 | Hum Reprod2004，10，1： | 1 |
| 13 | Semen quality in sub-fertile range for a significant proportion of young men from the general German population:a co-ordinated,controlled study of 791 men from Hamburg and Leipzig显示文摘 | Paasch U Salzbrunn A Glander HJ | 2008 | Int J Androl2008，31，2： | 1 |
| 14 | The prevalence of domestic violence among women seeking abortion 显示文摘 | Glander SS Moore ML Michielutte R | 1998 | Obstet Gynecol1998，91，6： | 1 |
| 15 | Agedependent inhibinB concentration in relation to FSH and semen sample qualities :a study in 2448 men显示文摘 | GRUNEWALD S GLANDER HJ PAASCH U | 2013 | Reproduction2013，145，3： | 1 |
| 16 | Role of caspases in male infertility显示文摘 | Said TM Paasch U Glander H J | 2004 | Hum Reprod Update2004，10，1： | 1 |
| 17 | Lowe syndrome, a deficiency of phosphatidylinositol 4, 5 bisphosphate 5-phosphatase in the Golgi apparatus 显示文摘 | Suchy SF Olivos Glander IM Nussabaum RL | 1995 | Hum Mol Genet1995，4，： | 1 |
| 18 | Treating type 2 diabetes in renal insufficiency:the role of pioglitazone显示文摘 | Frtsche L Budde K Glander P | 2003 | Int J Clin Pharmacol Ther2003，41，10： | 1 |
| 19 | Destabilization of the acro- some results in release of phospholipase A2 from humam spermato- zoa and subsequent formation of lysophospholipids 显示文摘 | Lessig J Glander H J Schiller J | 2006 | Androlo- gia2006，38，2： | 1 |
| 20 | Treating type 2 diabetes in renal insufficiency: the role of pioglitazone显示文摘 | Frtsche L Budde K Glander P Einecke G | 2003 | Int J Clin Pharmacol Ther2003，41，10： | 1 |
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