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| 1 | Hwangryunhaedoktang exerts anti-inflammation on LPS-induced NO production by suppressing MAPK and NF-κB activation in RAW264.7 macrophages显示文摘OBJECTIVE: This study aimed to evaluate whether Hwangryunhaedoktang(HHT), a herbal compound, has an inhibitory effect on lipopolysaccharide(LPS)-induced inflammation in RAW264.7 macrophages.METHODS: The effects of HHT were evaluated by confirming nitric oxide(NO) production and expression of inducible NO synthase(iN OS) and mitogen-activated protein kinases(MAPKs) in LPS-stimulated RAW264.7 macrophages via the Griess assay, Western blotting, and real-time reverse transcription quantitative polymerase chain reaction. Western blot analyses and luciferase assays were used to evaluate whether HHT has an effect on the phosphorylation and translocation of nuclear factor-κB(NF-κB). The secretion and expression of tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) were determined via enzyme-linked immunosorbent assay and Western blot analyses.RESULTS: HHT suppressed LPS-induced NO production and expression of iN OS in a dose-dependent manner. Additionally, MAPKs activation was also attenuated via inhibition of phosphorylation of extracellular signal-regulated kinases 1/2, c-Jun N-terminal kinase and p38 which were related to inflammatory pathway. Furthermore, HHT also effectively attenuated NF-κB activation and its translocation to the nucleus, a process that is closely linked to inflammation. LPS normally induced the expression of inflammatory cytokines such as TNF-α and IL-6, but the secretion and expression of TNF-α and IL-6 were significantly attenuated by pretreating the cells with HHT.CONCLUSION: HHT suppressed LPS-induced NO production by blocking the activation of NF-κB and MAPK signaling pathways in RAW264.7 macrophages. Furthermore, HHT may have an anti-inflammatory effect by suppressing the LPS-induced secretion of TNF-α and IL-6. Therefore, the traditional herbal formula HHT might be a useful potential therapeutic agent for inflammation. | Byung Hyuk Han Yun Jung Lee Jung Joo Yoon Eun Sik Choi Seung Namgung Xian Jun Jin Da Hye Jeong Dae Gill Kang Ho Sub Lee | 2017 | Journal of Integrative Medicine2017，15，4： | 5 |
| 2 | Involvement of heme oxygenase-1 induction in anti-vascular inflammation effects of Xanthoceras sorbifolia in human umbilical vein endothelial cells显示文摘OBJECTIVE: To define the effects of Xanthoceras sorbifolia(EXS) on vascular inflammation and the mechanisms in endothelial cells.METHODS: Vascular protective effects of an ethanol extract of seeds from EXS(1-50 μg/mL) against tumor necrosis factor-α(TNF-α)-induced vascularinflammation were examined in human umbilical vein endothelial cells(HUVECs).RESULTS: EXS significantly decreased TNF-α-induced expression of cell adhesion molecules, such as intracellular adhesion molecule-1, vascular cell adhesion molecule-1, and endothelial cell selectin,in a dose-dependent manner. Pre-treatment with EXS significantly inhibited translocation and transcriptional activity of nuclear factor-κB(NF-κB) increased by TNF-α. EXS also significantly inhibited formation of intracellular reactive oxygen species(ROS). Moreover, the vascular protective effects of EXS were linked to up-regulation of heme oxygenase-1(HO-1) and nuclear factor E2-related factor-2(Nrf-2) expression. EXS-induced HO-1 expression was significantly decreased in SnPP(HO-1 inhibitor)-and HO-1 siRNA-treated cells, whereas an increase was found in cobalt protoporphyrin IX(CoPP)(HO-1 inducer)-treated cells. In addition, pretreatment with EXS increased HO-1 and Nrf-2 expression under TNF-α stimulation with or without N-acetyl-L-cysteine. Furthermore, the inhibitory effects of EXS on TNF-α-induced vascular inflammation were partially reversed in SnPP-and of HO-1siRNA-treated cells but increased by CoPP.CONCLUSION: These results suggest that EXS may have important implications for prevention of vascular complications associated with vascular inflammation by inhibition of the NF-κB/ROS pathway and activation of the Nrf-2/HO-1 pathway. | Jung Joo Yoon Byung Hyuk Han Eun Sik Choi Seung Namgung Da Hye Jeong Song Nan Jin Yun Jung Lee Dae Gill Kang Ho Sub Lee | 2018 | Journal of Traditional Chinese Medicine2018，38，6： | 4 |
| 3 | Validity and reliability of the Obserer's Assessment of Alertness/Sedation Scale:study with intravenous midazolam 显示文摘 | Chernik DA Gillings D Laine H | 1990 | J Clin Psychopharmacol1990，10，： | 1 |
| 4 | Validity and reliability of the observers assessment of alertness/se dation scale:study with intravenous midazolam显示文摘 | Chernik DA Gilling SD Laine H | 1990 | J Clin Psychopharmacol1990，10，4： | 1 |
| 5 | Validity and reliability of the observers assessment of alertness/sedation scale: study with intra- venous midazolam 显示文摘 | CHERNIK DA GILLINGS D LAINE H | 1990 | J Clin Psychopharmacol1990，10，： | 1 |
| 6 | Validity and reliability of the Observer's Assessment of Alertness/Sedation Scale: a study with intravenous midazolam 显示文摘 | Chemik DA Gillings D Laine H | 1990 | J Clin Psychopharmacol1990，10，： | 1 |
| 7 | Validity and relia-bility of the Observer’s Assessment of Alertness/ SedationScale: study with intravenous midazolam 显示文摘 | Chemik DA Gillings D Laine H | 1990 | J Clin Psycho-pharmacol1990，10，4： | 1 |
| 8 | Validity and reliability of the Observer's Assessment of Alertness/Sedation Scale: study with intra- venous midazolam 显示文摘 | Chernik DA Gillings D Laine H | 1990 | J Clin Psychopharmacol1990，10，： | 1 |
| 9 | Validity and reliablity and reliability of the obsrvers assessment of alemess sedation scale:Study with intravenous midazolam显示文摘 | Chemik DA Gillings D Laine H | 1990 | J Clin Psychopharmacol1990，10，2： | 1 |
| 10 | Vaidity and reliability of the Observer's Assessment of Alterness/Sedation Scale: study with intravenous midazolam 显示文摘 | Chernik DA Gilling D Lane H | 1990 | J Clin Psyehopharmacol1990，10，4： | 1 |
| 11 | Validity and reliability of the Observer's Assessment of Alertness/Sedation Scale:study with intravenous midazolam显示文摘 | Chernik DA Gillings D | 1990 | Clin Psychopharmacol1990，，10： | 1 |
| 12 | Validity and reliabi- lity of the observer's assessment of alertness/sedation scale: study with intravenous midazolam显示文摘 | Chernik DA Gillings D Laine H | 1990 | J Clin Psycho Pharma- col1990，10，4： | 1 |
| 13 | Validity and reliability of the Observer's Assessment of Alertness/Sedation Scale: study with intravenous midazolam 显示文摘 | Chernik DA Gillings D Laine H | 1990 | J Clin Psychopharmacol1990，10，4： | 1 |
| 14 | Validity and reliability of theObserver’s Assessment of Alertness/Sedation Scale:study with intra-venous midazolam显示文摘 | Chemik DA Gillings D Laine H | 1990 | J Clin Psychopharmacol1990，10，4： | 1 |
| 15 | Validity and reliability of the observers assessment of alertness/sedation scale : Study with in- travenous midazolam 显示文摘 | Chemik DA Gillings D Laine H | 1990 | J Clin Psychopharmaeol1990，10，： | 1 |
| 16 | Validity and reliability of the observers assessment of alertness/sedationscale: study with intravenous midazolam显示文摘 | Chemik DA Gillings D Laine H | 1999 | J Clin Psychopharmacol1999，10，4： | 1 |
| 17 | Validity and reliability of the Observer's Assessment of Alertness/Sedation Scale:study with intravenous midazolam显示文摘 | Chernik DA Gillings D Laine H | 1990 | J Clin Psychopharmacol1990，10，4： | 1 |
| 18 | Validity and reliability of the Observer's Assessment of Alertness/Sedation Scale: study with intravenous midazolam 显示文摘 | Chemik DA Gillings D Laine H | 1990 | J Clin Psychopharmacol1990，10，4： | 1 |
| 19 | Salvage stereotactic body radiotherapy for locally recurrent non-small cell lung cancer after sublobar resection and I (125) vicryl mesh brachytherapy显示文摘 | Gill BS Clump DA Burton SA | 2015 | Front Oncol2015，5，： | 1 |
| 20 | Validity and relialility of the observer's assessment of alertness/sedation scale:study with intravenous midazolam显示文摘 | Chernik DA Gillings D Laine H | 1990 | J Clin Psychopharmacol1990，1，： | 1 |
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