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| 1 | Intrahepatic transplantation of hepatic oval cells for fulminant hepatic failure in rats显示文摘AIM:To evaluate the effect of intrahepatic transplantation of hepatic oval cells(HOC)on fulminant hepatic failure(FHF)in rats.METHODS:HOC obtained from rats were labeled with green fluocescent protein(GFP)or 5,6carboxyfluorescein diacetate succinmidyl ester(CFDASE).Cell fluorescence was observed under fluorescent microscope at 6,24,48 and 72 h after labeling.CFDASE labeled HOC(5×10 6 cells each rat)were injected into livers of rats with FHF induced by D-galactosamine.Serum albumin(ALB),alanine aminotransferase(ALT),aspartate aminotransferase(AST)and total bilirubin(TBil)levels were measured at different time points.Liver function of rats was examined on days 3,7,14 and 21 after HOC transplantation.RESULTS:The positive rate of GFP and CFDA-SE labeled HOC was 10%and 90%,respectively,with no significant change in cell viabilities.The survival rate was higher in HOC transplantation group than in control group,especially 48(9/15 vs 6/15)and 72 h(9/15 vs 4/15)after HOC transplantation.The serum ALT,AST and TBil levels were decreased while the serum Alb level was increased after HOC transplantation.Fluorescence became faded and diffused in liver tissues,suggesting that proliferation and differentiation occur in transplanted HOC.CONCLUSION:CFDA-SE is superior to GFP in labeling HOC,although fluorescence intensity is decreased progressively with cell division.HOC transplantation can improve the liver function and increase the survival rate of recipients. | Chen-Xuan Wu,Qi Zou,Zheng-Yan Zhu,Ying-Tang Gao,Yi-Jun Wang,Department of Hepatobiliary,The Third Center of Affiliated Hospital of Tianjin Medical University Key Laboratory of Artificial Cells of Tianjin,Tianjin 300170,China Author contributions:Wang YJ,Wu CH and Zou Q designed the research Zou Q,Zhu ZY,Gao YT performed the research Zou Q analyzed the data Wang YJ and Wu CX wrote the paper.Supported by Tianjin Science Committee,Grant No.05SYSYJC02600 and Tianjin Health Bureau,Grant No.05KYR01 | 2009 | World Journal of Gastroenterology2009，15，12： | 7 |
| 2 | Purification and characterization of pyrophosphatase from bighead carp ( Aristichthys nobilis) 显示文摘 | Gao RC Xue CH | 2008 | Swiss Soci Food Sci Technol2008，41，2： | 1 |
| 3 | The expression of LytM is down-regulated by RNAⅢ in Staphylococcus aureus显示文摘 | Mu CH Liu Y Gao YP | 2012 | Journal of Basic Microbiology2012，52，6： | 1 |
| 4 | A protein delivery system : biodegradable atginate-chitosan-poly ( lactic-co-glycolic acid) composite microspheres 显示文摘 | ZHENG CH GAO JQ ZHANG YP | 2004 | Biochem Biophys Res Commun2004，323，4： | 1 |
| 5 | An outbreak of the desert sub-type of zoonotic visceral Leishmaniasis in Jiashi, Xinjiang Uygur Autonomous Region, People's Republic of China显示文摘 | Wang JY Gao CH Yang YT | 2010 | Parasitol Int2010，59，3： | 1 |
| 6 | Effects of additives and processing parameters on the initial burst release of protein from poly ( lactic-co-glycolic acid ) microspheres 显示文摘 | ZHENG CH GAO JQ LIANG WQ | 2006 | PDA J PharmSciTechnol2006，60，1： | 1 |
| 7 | Superparamagnetic iron oxide nanoparticles:from preparations to in vivo MRI applications显示文摘 | Qiao RR Yang CH Gao MY | 2009 | J Mater Chem2009，19，： | 1 |
| 8 | Mangiferin, an anti- HIV-t agent targeting protease and effective against resistant strains显示文摘 | Wang RR Gao YD Ma CH | 2011 | Molecules2011，16，5： | 1 |
| 9 | 5' flanking sequence and genomic structure of Egr-1, a murine mitogen inducible zinc finger encoding gene 显示文摘 | Tsai-Morris CH Gao XM Sukhatme VP | 1988 | Nucleic Acids Res1988，16，18： | 1 |
| 10 | A cotton (Gossypium hirsutum ) DRE-binding transcription factor gene, GhDREB, confers enhanced tolerance to drought, high salt, and freezing stresses in transgenic wheat显示文摘 | GAO S Q CH EN M XIA L Q | 2009 | Plant Cell Reports2009，28，2： | 1 |
| 11 | Three - dimensional analysis of rows of piles as passive barriers for ground vibration isolation 显示文摘 | Gao G Y Li Z Y Qiu CH | 2006 | Soil Dynamics and Earthquake Engineering2006，26，11： | 1 |
| 12 | Real-time three-dimensional echocardiographic determination of right ventricular outflow tract high septal pacing sites 显示文摘 | Gao CH Zhang H Cui JY | 2012 | Eur Heart J Cardiovasc Imaging2012，13，1： | 1 |
| 13 | Identification and characterization of an IkappaB kinase显示文摘 | Regnier CH Song HY Gao X | 1997 | Cell1997，90，2： | 1 |
| 14 | The application analysis and discussion of refined care in elderly cadre's ward显示文摘 | Gao CH | 2010 | Jilin Med J2010，31，35： | 1 |
| 15 | 经皮冠状动脉介入后阻塞性睡眠呼吸暂停与心血管事件的关系显示文摘目前关于阻塞性睡眠呼吸暂停(obstructive sleepapnea,OSA)对冠状动脉疾病患者的预后意义的大型队列研究数据匮乏。该研究假设OSA可预测行经皮冠状动脉介入治疗(percutaneous coronary intervention.PCI)患者未来主要不良心脏和脑血管事件(major adverse cardiac and cerebrovascularevents,MACCE)。方法:睡眠及支架研究是一项前瞻性、多中心注册研究,纳入2011年12月和2014年4月来自5个国家经PCI治疗成功的患者1748例。 | Lee CH Sethi R Li R Ho HH Hein T Jim MH Loo G Koo CY Gao XF Chandra S Yang XX Furlan SF Ge Z Mundhekar A Zhang WW Ucha CH Kharwar RB Chan PF Chen SL Chan MY Richards A Tan HC Ong TH Roldan G Tai BC Drager LF Zhang JJ 刘莉 叶鹏 | 2016 | 中华高血压杂志2016，24，5： | 1 |
| 16 | Molecular analysis of human forearm superficial skin bacterial biota 显示文摘 | Gao Z Tseng CH Pei ZH Blaser MJ | 2007 | Proc Natl Acad Sci USA2007，104，8： | 1 |
| 17 | Prenatal exposure to mercury and neurobehavioral development of neonates in Zhoushan City,China显示文摘 | Yan CH Tian Y | 2007 | Environ Res2007，105，3： | 1 |
| 18 | Identification and characterization of an IKB kinase显示文摘 | Regnier CH Song HY Gao Xiong | 1997 | Cell1997，90，： | 1 |
| 19 | Prenatal exposure to mercury and neurobehavioral development of neonates in Zhoushan city 显示文摘 | Gao Y Yah CH Tian Y | 2007 | Environ Res2007，105，3： | 1 |
| 20 | Characterization and antitumor activity of a polysaccharide from Strongylocentrotus nudus eggs显示文摘 | Liu CH Lin Q X Gao Y | 2007 | Carbohydrate Polymers2007，67，： | 1 |
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